The Development of Reproducible and Selective Uric Acid Biosensor by Using Electrodeposited Polytyramine as Matrix Polymer

Abstract

A versatile method for the construction of reproducible and high selective uric acid biosensor is explained. Electrodeposited polytyramine is used as biosensor matrixes due to its compatibility to immobilize enzyme uric

The Design of Sensitive, Selective and Reproducible Biosensor With Enzyme Immobilisation For Analytical Applications

“…The uric acid standard solution and the sample is dissolved in 4% lithium carbonate solution to allow the uric acid dissolved in aqueous solution. The determination of uric acid proceeds at optimum experimental conditions [37,38].…”

“…The calibration slope was 0.0661 µA mM -1 , limit detection is 0.001 mM uric acid where the signal to noise ratio is three (S/N=3). This typical wide linearity detection range has been adequate to be applied for the determination of uric acid in real samples without pretreatment, except the dilution with lithium carbonate for very concentrated samples, as well as dissolving the uric acid in aqueous solution [37]. The calibration plot presented in Figure 2b is the result of multiple measurements of standards uric acid solutions from five enzyme electrodes.…”

“…A biosensor with immobilization of enzyme in polytyramine membrane film is used as an example for a sensitive, selective and reproducible biosensor for uric acid assay. The biosensor design is based upon successful development explained previously [37]. The uric acid determination is based on enzymatic catalytic reaction (1) and (2).…”

Electrodeposited Carbonyl Functional Polymers as Suitable Supports for Preparation of the First-Generation Biosensors

“…Generally, enzyme immobilization via a covalent bonding to an insoluble conductive polymer electrodeposited onto the surface of electrochemical transducers (electrodes) guarantees high mechanical stability and reproducible serial production, in contrast to the physical anchoring of enzyme molecules (via so-called encapsulation) into the net structure of a polymer, without need to engage functional groups that mediate the covalent chemical bonding [ 1 ]. Hence, it can be assumed that it is only a matter of time until, for example, electrodeposited amino-functional polymers such as polytyramine [ 2 ], polyhistamine [ 3 ], and polyarginine [ 4 ] will find their use in enzyme immobilization, like the already commonly used chitosan [ 5 ], poly(ethylene glycol) diglycidyl ether (PEGDE) [ 6 ], poly(ethylene glycol) diamine, polyallylamine, or poly- l -arginine, which are substrates applicable using a simple drop-casting method [ 7 ].…”