The Development of Peptide-based Antimicrobial Agents against Dengue Virus

Huang, Yen-Wei; Lee, Chun-Ting; Wang, Ta-Chen; Kao, Yun-Chung; Yang, Chih-Hui; Lin, Yumei

doi:10.2174/1389203719666180531122724

Cited by 7 publications

(5 citation statements)

References 154 publications

(171 reference statements)

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“…In contrast to antimicrobials that target proteins, cells cannot readily build resistance to AMPs that target the cell membrane because it is difficult, if not impossible, to alter this crucial cell structure [ 2 ]. Antimicrobial peptides have been shown to have antiviral [ 3 ] and anticancer [ 4 ] activities as well. Currently (May 2018), there are 2981 AMPs curated by the APD3 database [ 5 ], in which more than 1000 of them are peptides from amphibians and about 200 are human host defense peptides.…”

Section: Introductionmentioning

confidence: 99%

Membrane Active Peptides and Their Biophysical Characterization

2018

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In the last 20 years, an increasing number of studies have been reported on membrane active peptides. These peptides exert their biological activity by interacting with the cell membrane, either to disrupt it and lead to cell lysis or to translocate through it to deliver cargos into the cell and reach their target. Membrane active peptides are attractive alternatives to currently used pharmaceuticals and the number of antimicrobial peptides (AMPs) and peptides designed for drug and gene delivery in the drug pipeline is increasing. Here, we focus on two most prominent classes of membrane active peptides; AMPs and cell-penetrating peptides (CPPs). Antimicrobial peptides are a group of membrane active peptides that disrupt the membrane integrity or inhibit the cellular functions of bacteria, virus, and fungi. Cell penetrating peptides are another group of membrane active peptides that mainly function as cargo-carriers even though they may also show antimicrobial activity. Biophysical techniques shed light on peptide–membrane interactions at higher resolution due to the advances in optics, image processing, and computational resources. Structural investigation of membrane active peptides in the presence of the membrane provides important clues on the effect of the membrane environment on peptide conformations. Live imaging techniques allow examination of peptide action at a single cell or single molecule level. In addition to these experimental biophysical techniques, molecular dynamics simulations provide clues on the peptide–lipid interactions and dynamics of the cell entry process at atomic detail. In this review, we summarize the recent advances in experimental and computational investigation of membrane active peptides with particular emphasis on two amphipathic membrane active peptides, the AMP melittin and the CPP pVEC.

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Section: Introductionmentioning

confidence: 99%

Membrane Active Peptides and Their Biophysical Characterization

2018

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“…In addition to 2FOM, these compounds also possessed binding affinity towards 2j7u (NS5 polymerase) and 1OKE (envelope protein). NS5 polymerase is involved in virus replication by the capping of RNA and host cell gene regulation [32], while envelope protein is the mediator for viral entry and membrane fusion with a receptor on the host cell [33]. The binding of these identified compounds with NS5 polymerase and envelope protein may hinder viral replication and prevent further spread of dengue infection.…”

Section: Discussionmentioning

confidence: 99%

Antiviral Activities of Streptomyces KSF 103 Methanolic Extracts Against Dengue Virus Type-2

Zulkifli¹,

Khairat²,

Azman³

et al. 2023

Preprint

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Dengue has long been a serious health burden to the global community, especially for those living in the tropics. In spite of the availability of vaccines, effective treatment for the infection is still needed and currently remained absent. In the present study, antiviral properties of the KSF 103 ME which consisted of a number of potential antiviral compounds were investigated against DENV-2. The effects of this extract against DENV-2 replication were determined using the qRT-PCR. Findings from the study suggested that the KSF 103 ME showed maximum inhibitory properties toward the virus during the virus entry stage at concentrations of more than 12.5 µg/mL. Minimal antiviral activities were observed at other virus replication stages; adsorption (42% reduction at 50 µg/mL), post-adsorption (67.6% reduction at 50 µg/mL), prophylactic treatment (68.4% and 87.7% reductions at 50 µg/mL and 25 µg/mL, respectively) and direct virucidal assay (48% and 56.8% reductions at 50 µg/mL and 25 µg/mL, respectively. The KSF 103 ME inhibited dengue virus repication with an IC50 value of 20.3 µg/mL and SI value of 38.9. The KSF 103 ME showed potent antiviral properties against DENV during the entry stage. Further studies will be needed to deduce the antiviral mechanisms of the KSF 103 ME against DENV.

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“…[6][7][8] Many researches have been conducted to search for DENV vaccines or drugs. [11][12][13][14][15][16] Nevertheless, there are still no effective and specific vaccines or drugs to treat DENV infection, implied by the ever-increasing prevalence of DENV worldwide and also in Indonesia. [2,11,[13][14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

Potential of Peptide-Based Non-Structural Protein 1 (NS1) Inhibitor in Obstructing Dengue Virus (DENV) Replication

Asyura¹,

Fauzi²,

Ayub

2021

GMJ

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Introduction: Dengue Virus (DENV) is the pathogen for human dengue fever and is responsible for 390 million infections per year. The viral genome produces about 10 viral protein products, one of them being NS1. The NS1 protein plays a key role in viral replication and stimulation of humoral immune cells, thus being the perfect candidate to create an effective antiviral drug or vaccine for dengue Methods: Dengue Virus (DENV) is the pathogen for human dengue fever and is responsible for 390 million infections per year. The viral genome produces about 10 viral protein products, one of them being NS1. The NS1 protein plays a key role in viral replication and stimulation of humoral immune cells, thus being the perfect candidate to create an effective antiviral drug or vaccine for dengue Conclusion: The review established promising results of using peptide-based intervention on NS1. Further in vivo and randomized controlled trials are advised to solidify the applicability and biosafety of the intervention

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The Development of Peptide-based Antimicrobial Agents against Dengue Virus

Cited by 7 publications

References 154 publications

Membrane Active Peptides and Their Biophysical Characterization

Membrane Active Peptides and Their Biophysical Characterization

Antiviral Activities of Streptomyces KSF 103 Methanolic Extracts Against Dengue Virus Type-2

Potential of Peptide-Based Non-Structural Protein 1 (NS1) Inhibitor in Obstructing Dengue Virus (DENV) Replication

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