The development of molecular fluorescent switches

Silva, A.Prasanna de; Fox, David B.; Moody, Thomas S.; Weir, Sheenagh M.

doi:10.1016/s0167-7799(00)01513-4

Cited by 113 publications

(91 citation statements)

References 17 publications

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“…Intensity-based probes are 'turn-on' fluorophores, and display a chelation-dependent increase in fluorescence intensity of up to 100-fold. Most of these probes are based on the modulation in the photon-induced electron transfer phenomenon (16)(17)(18) . Briefly, the probes are composed of a fluorophore, a spacer domain and an electron-rich metal chelate.…”

Section: Imaging Free Zn 2+ In Living Cellsmentioning

confidence: 99%

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

et al. 2015

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Zinc is an important micronutrient, essential in the diet to avoid a variety of conditions associated with malnutrition such as diarrhoea and alopecia. Lowered circulating levels of zinc are also found in diabetes mellitus, a condition which affects one in twelve of the adult population and whose treatments consume approximately 10 % of healthcare budgets. Zn 2+ ions are essential for a huge range of cellular functions and, in the specialised pancreatic β-cell, for the storage of insulin within the secretory granule. Correspondingly, genetic variants in the SLC30A8 gene, which encodes the diabetes-associated granule-resident Zn 2+ transporter ZnT8, are associated with an altered risk of type 2 diabetes. Here, we focus on (i) recent advances in measuring free zinc concentrations dynamically in subcellular compartments, and (ii) studies dissecting the role of intracellular zinc in the control of glucose homeostasis in vitro and in vivo. We discuss the effects on insulin secretion and action of deleting or overexpressing Slc30a8 highly selectively in the pancreatic β-cell, and the role of zinc in insulin signalling. While modulated by genetic variability, healthy levels of dietary zinc, and hence normal cellular zinc homeostasis, are likely to play an important role in the proper release and action of insulin to maintain glucose homeostasis and lower diabetes risk.

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Section: Imaging Free Zn 2+ In Living Cellsmentioning

confidence: 99%

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

et al. 2015

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“…So on the one hand, the success of wet molecular logic should not be surprising. On the other hand, it should also be natural for such logic devices to find ready application in physiology, [6] medicine [7] and biotechnology [8] as sensors [9] and diagnostic systems. When such applications target cell interiors, logically enabled molecules leave classical semiconductor devices behind.…”

Section: Introductionmentioning

confidence: 99%

Molecular‐Scale Logic Gates

Silva

McClenaghan

2004

Chemistry A European J

595

240

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Currently available approaches to molecular-scale logic gates are summarized and compared. These include: chemically-controlled fluorescent and transmittance-based switches concerned with small molecules, DNA oligonucleotides with fluorescence readout, oligonucleotide reactions with DNA-based catalysts, chemically-gated photochromics, reversibly denaturable proteins, molecular machines with optical and electronic signals, two-photon fluorophores and multichromophoric transient optical switches. The photochemical principles of electron and energy transfer are involved in several of these approaches. More complex molecular logic systems with reconfigurability and superposability provide contrasts with current semiconductor electronics. Integration of simple logic functions to produce more complex ones is also discussed in terms of recent developments.

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“…The considerable increase in the fluorescent intensities as an effect of Zn 2+ , Cd 2+ , Ca 2+ and Mg 2+ ion addition is in accord with a hindered PET process caused by complexation, decreasing the HOMO energy level of the coordination site. 4,38,[42][43][44][45] Copper ions are capable of facilitating the electrontransfer of the PET mechanism, thereby resulting in fluorescence quenching. The fluorescent spectra of none of the compounds changed considerably (Fig.…”

Section: The Uv-vis and Fluorescent Characterization Of Compoundsmentioning

confidence: 99%