The Development of<i>Diaporthe Phaseolorum</i>Variety<i>Sojae</i>in Culture

Jensen, Jon D.

doi:10.1080/00275514.1983.12023794

Cited by 12 publications

(1 citation statement)

References 27 publications

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“…Mature ascospores and asci were isolated from individual perithecia that were horizontally dissected, and the centrum macerated in sterile distilled water (Luttrell, 1951;Jensen, 1983). A series of dilute spore suspensions were washed onto the surface of 2% water agar (WA) plates or WA acidified to pH 4 .…”

Section: Source Of Strainsmentioning

confidence: 99%

Vegetative incompatibility in Diaporthe ambigua

Smit

Wingfield²,

Wingfield³

1997

Plant Pathology

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Vegetative compatibility of strains of Diaporthe ambigua has not previously been examined. Single ascospore and single ascus strains, originating from individual apple, pear and plum rootstocks, were paired on freshly prepared oatmeal agar to determine if vegetative incompatibility could be detected in D. ambigua. Barrage reactions were evident as black lines along the zone of mycelial contact between expanding colonies (vegetative incompatibility reaction). Strains from cankers within an area were of numerous vegetative compatibility groups (VCGs). Strains from adjacent rootstocks usually differed in VCG. D. ambigua has the ability to outcross, and does so, despite its apparent homothallic nature. DsRNA-containing strains of D. ambigua developed a broad, clear zone when paired with a dsRNA-containing strain from a different VCG.

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Section: Source Of Strainsmentioning

confidence: 99%

Vegetative incompatibility in Diaporthe ambigua

Smit

Wingfield²,

Wingfield³

1997

Plant Pathology

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Pyrenomycetes — Fungi with Perithecia

Samuels

Blackwell

2001

Systematics and Evolution

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First report of an endophyte (Diaporthe phaseolorum var. sojae) from Kandelia candel

Cheng

Tang

et al. 2008

Journal of Forestry Research

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Mangrove endophytic fungus 1893 was isolated from Kandelia candel from an estuarine mangrove on the South China Sea Coast. Two new lactones 1893A and 1893B, together with other known compounds, have been isolated from its fermentation broth. To classify the endophyte correctly for further industrial application, a combination of morphological and molecular techniques was used to approach its identity. The endophyte was compared with similar species having trichogynes or trichogyne-like hyphae which apparently fused with antheridium-like hyphae, and perithecia initials developing from an ascogonial coil surrounded by enveloping hyphae in early developmental stages on pure culture. Further morphological characteristics on host and non-host were used for comparison with similar species when the endophyte was cultivated on leaves of Kandelia candel and Mangifera indica, respectively, which resulted in classifying the endophyte as a Phomopsis species. The ITS sequence of rDNA was used to infer its phylogenetic relationships with Phomopsis species that resembled the strain in morphology or ecology. Finally, the endophyte was identified as Diaporthe phaseolorum var. sojae based on morphological and molecular evidence. Our study is a first report of Diaporthe phaseolorum var. sojae isolated from mangrove Kandelia candel.

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The Development ofDiaporthe PhaseolorumVarietySojaein Culture

Cited by 12 publications

References 27 publications

Vegetative incompatibility in Diaporthe ambigua

Vegetative incompatibility in Diaporthe ambigua

Pyrenomycetes — Fungi with Perithecia

First report of an endophyte (Diaporthe phaseolorum var. sojae) from Kandelia candel

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