First report of an endophyte (Diaporthe phaseolorum var. sojae) from Kandelia candel

Abstract

Mangrove endophytic fungus 1893 was isolated from Kandelia candel from an estuarine mangrove on the South China Sea Coast. Two new lactones 1893A and 1893B, together with other known compounds, have been isolated from its fermentation broth. To classify the endophyte correctly for further industrial application, a combination of morphological and molecular techniques was used to approach its identity. The endophyte was compared with similar species having trichogynes or trichogyne-like hyphae which apparently fused with antheridium-like hyphae, and perithecia initials developing from an ascogonial coil surrounded by enveloping hyphae in early developmental stages on pure culture. Further morphological characteristics on host and non-host were used for comparison with similar species when the endophyte was cultivated on leaves of Kandelia candel and Mangifera indica, respectively, which resulted in classifying the endophyte as a Phomopsis species. The ITS sequence of rDNA was used to infer its phylogenetic relationships with Phomopsis species that resembled the strain in morphology or ecology. Finally, the endophyte was identified as Diaporthe phaseolorum var. sojae based on morphological and molecular evidence. Our study is a first report of Diaporthe phaseolorum var. sojae isolated from mangrove Kandelia candel.

The Diaporthe sojae species complex: Phylogenetic re-assessment of pathogens associated with soybean, cucurbits and other field crops

“…Although many of the species included in study are well known as pathogens of important crops, the extensive sampling from various geographic locations and different hosts has revealed that D. longicolla and D. sojae can be endophytic in tropical trees and mangroves (Cheng et al 2008;Sebastianes et al 2012;R€ onsberg et al 2013). The closely related taxa D. endophytica, Diaporthe schini, and Diaporthe terebinthifolii were originally described from Schinus in Brazil as endophytes (Gomes et al 2013).…”

The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens

“…Tuset & Portilla, a pathogen associated with shoot blight of almond and peach has been recorded from the asymptomatic nursery plant of Vitis vinifera in South Africa. In another case, D. phaseolorum, the causative agent of diseases of soybean has been reported as endophytes in the estuarine mangrove plant Kandelia candel (Cheng et al 2006). range of plant hosts ( Fig.…”

Resolving the Diaporthe species occurring on soybean in Croatia

“…Known hosts — Abutilon theophrasti ( Hepperly et al 1980 ), Allium cepa , Allium sativum , Arachis hypogaea , Capsicum frutescens , Hibiscus esculentus , Lespedeza sp., Lupinus hirsutus , Lycopersicon esculentum , Phaseolus vulgaris , Strophostyles helvola and Vigna sinensis ( Hanlin 1963 ), Amaranthus spinosus and Leonorus sibiricus ( Cerkauskas et al 1983 ), Arctium lappa , Camptotheca acuminata ( Chang et al 2005 ), Capsicum annuum ( Pennycook 1989 ), Glycine max ( Petch 1922 , Preston 1945 , Gilman 1949 , Johnson & Kilpatrick 1953 , Gerdemann 1954 , Peregrine & Siddiqi 1972 , Kmetz et al 1974 , Gorter 1977 , Ersek 1978 , Ebbels & Allen 1979 , Bernaux 1981 , Kozireva et al 1982 , Alfieri et al 1984 , Jasnić & Vidić 1985 , Richardson 1990 , Dahal et al 1992 , Zhang et al 1997 ), Helianthus annuus ( Vrandečić et al 2009 ), Ipomoea batatas , Phaseolus lunatus , Phaseolus sp., Solanum tuberosum and Zea mays ( Wehmeyer 1933 ), Kandelia candel ( Cheng et al 2008 ) and Stokesia laevis ( Sogonov et al 2008 ).…”