Resolving the Diaporthe species occurring on soybean in Croatia

Abstract

Diaporthe (anamorph = Phomopsis) species are plant pathogens and endophytes on a wide range of hosts including economically important crops. At least four Diaporthe taxa occur on soybean and they are responsible for serious diseases and significant yield losses. Although several studies have extensively described the culture and morphological characters of these pathogens, their taxonomy has not been fully resolved. Diaporthe and Phomopsis isolates were obtained from soybean and other plant hosts throughout Croatia. Phylogenetic relationships were determined through analyses of partial translation elongation factor 1-alpha (EF1-α) gene and ITS nrDNA sequence data. By combining morphological and molecular data, four species could be distinguished on soybeans in Croatia. Diaporthe phaseolorum is described in this study and its synonyms are discussed. Diaporthe phaseolorum var. caulivora is raised to species status and the name Diaporthe caulivora is introduced to accommodate it. A species previously known as Phomopsis sp. 9 from earlier studies on sunflower, grapevine, rooibos and hydrangea is reported for the first time on soybean, and is formally described as Diaporthe novem. The well-known soybean pathogen Phomopsis longicolla was also collected in the present study and was transferred to Diaporthe longicolla comb. nov. The presence of these species on herbaceous hosts raises once more the relevance of weeds as reservoirs for pathogens of economically important plants.

Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex

“…The Apn2 region has recently been used in conflicting genera like Colletotrichum (Crouch and Tomaso-Peterson 2012;Silva et al 2012b;Doyle et al 2013;Sharma et al 2013) and the Apn2 and Apn2/MAT-IGS (intergenic spacer between 3' end of the DNA lyase and mating type locus MAT1-2) genetic markers recommended as a better marker in disentangling the C. gloeosporioides species complex (Silva et al 2012a, b). Mating type genes of Diaporthe were amplified in several previous studies and utilised in phylogenetic analyses (Santos et al 2010(Santos et al , 2011. Portions of the α-1 box in MAT 1-1-1 gene (141 bp) and a portion of HMG domain of MAT 1-2-1 (229 bp) regions were shown to have less utility as phylogenetic markers than for screening mating types of isolates (Santos et al 2010).…”

“…The genus Diaporthe comprises pathogenic, endophytic and saprobic species with both temperate and tropical geographic distributions (Rehner and Uecker 1994;Rossman et al 2007;Udayanga et al 2011;Huang et al 2013). Species recognition criteria in Diaporthe have evolved from morphology and host associations (Wehmeyer 1933) to the recent use of phylogenetic species recognition (Castlebury et al 2003;Santos and Phillips 2009;Santos et al 2011;Udayanga et al 2012a, b;Gomes et al 2013;Tan et al 2013).…”

Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China

“…Diaporthe species can cause blights, cankers, decay and wilt, dieback, leaf spots, and root and fruit rots (Mostert et al 2001;Anagnostakis 2007;Santos et al 2011;Thompson et al 2011;Fan et al 2015;Lawrence et al 2015). The names Diaporthe and Phomopsis are no longer used for different morphs of the same genus (Santos and Phillips 2009).…”

“…Diaporthe species are associated with wide range of hosts and are commonly encountered as pathogens, endophytes, or saprobes of crops, ornamentals and forest trees (Brayford 1990, Mostert et al 2001Farr et al 2002;Santos and Phillips 2009;Santos et al 2011;Udayanga et al 2011Udayanga et al , 2012Udayanga et al , 2014aUdayanga et al , 2015Gomes et al 2013;Hyde et al 2014;Fan et al 2015). Diaporthe species can cause blights, cankers, decay and wilt, dieback, leaf spots, and root and fruit rots (Mostert et al 2001;Anagnostakis 2007;Santos et al 2011;Thompson et al 2011;Fan et al 2015;Lawrence et al 2015).…”

Phylogeny and morphology reveal two new species of Diaporthe from Traditional Chinese Medicine in Northeast China

“…For mycologists, studying on phytopathogenic Diaporthe species are therefore particularly important to work on a wide range of crops and economic trees (e.g. grapes, sunflowers, soybean and various diseases associated with ornamentals and forest trees) (Santos et al 2011, Thompson et al 2011, Baumgartner et al 2013, Fan et al 2015, Du et al 2016, Yang et al 2017a.…”