The development of a tissue-engineered artery using decellularized scaffold and autologous ovine mesenchymal stem cells

Mechanical characterization of a customized decellularized scaffold for vascular tissue engineering

“…Subsequently in a large patient cohort this necessitates the need for a suitable alternative to autologous vessel grafting for small diameter applications (<6mm diameter). Substantial progress has been made in the field of tissue engineering to address this clinical need (L'Heureux et al, 2006, Niklason et al, 1999, Zhao et al, 2010, but there still remains some limitations to its progress: obtaining suitable architecture, appropriate mechanical properties, sufficient smooth muscle cell (SMC) density and correct phenotype are still major challenges (Tranquillo, 2002, Villalona et al, 2010, Yazdani et al, 2009). …”

In vitro recellularization of aorta scaffolds prepared by sonication treatment

“…It is followed b decellularized tissues within the re designed O-ring shaped reservoir ha of cell infiltration onto decellulariz the cell suspension was maintained within the area of O-ring shaped r prevent the cell suspension from dra cell-seeding. In addition, the use of r of nutrients and oxygen freely by p was a very small distance between c scaffolds [14]. Hence, it is shown self-designed O-ring shaped reserv the efficiency of cell infiltration and Compared to other seeding met seeding, cell suspension injection, seeding method from previously pu proposed self-designed O-ring shape infiltration depth.…”

“…This is further con immunofluorescence image of seeded scaffo monolayer was observed to deposit on the s acellular dermis. Prior to limitation of sta previous literature has demonstrated a modi seeding with the use of silicon ringlets [14]. T ringlets during seeding of decellularized ovin have improved the exchange of nutrients an preventing the collapsing and narrowing o absorption of culture medium.…”

Preparation of a nano- and micro-fibrous decellularized scaffold seeded with autologous mesenchymal stem cells for inguinal hernia repair

“…Strength retention of the scaffold was measured to determine whether the grafts could withstand the pressure forces of intra-abdominal cavity, which ranges from 1 to 6.5 mmHg in a normal human being. 24,25 The strength of the DA was different -but not to a significant degree -from that of the native tissue, which can withstand pressures of about 2,000 mmHg, 19 suggesting that the scaffold had enough strength to withstand abdominal forces. lDh assay Figure 2H demonstrated the total LDH released from MSCs after 1, 3, 5, and 7 days of culture on DA scaffolds.…”

“…19,20 Cells from the third passage were trypsinized, washed twice, and passed through a 10-µm filter (Millipore) to collect the small-sized cells.…”