The development dynamics of the maize root transcriptome responsive to heavy metal Pb pollution

Gao, Jian; Zhang, Yongzhong; Lu, Chaolong; Peng, Hua; Luo, Mao; Li, Gaoke; Shen, Yaou; Ding, Haiping; Zhang, Zhiming; Pan, Guangtang; Lin, Hung-Ching

doi:10.1016/j.bbrc.2015.01.101

Cited by 23 publications

(14 citation statements)

References 28 publications

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“…We selected the non-hyperaccumulator maize germplasm 9782 with Pb accumulation in roots, stems, and leaves under Pb conditions, and compared these individuals with the hyperaccumulator 178 (Zhang et al, 2012). In addition, transcriptomic changes during maize root development in response to Pb were investigated in the non-hyperaccumulator maize germplasm 9782 using RNA sequencing (Gao et al, 2015), as well as the dynamics of DNA methylation in maize germplasm 9782 roots under Pb stress (Ding et al, 2014). However, proteomic alteration profiling involved in various metabolic functions associated with Pb pollution is still uncertain.…”

Section: Introductionmentioning

confidence: 99%

Proteomic changes in maize as a response to heavy metal (lead) stress revealed by iTRAQ quantitative proteomics

Gao

Peng

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Lead (Pb), a heavy metal, has become a crucial pollutant in soil and water, causing not only permanent and irreversible health problems, but also substantial reduction in crop yields. In this study, we conducted proteome analysis of the roots of the non-hyperaccumulator inbred maize line 9782 at four developmental stages (0, 12, 24, and 48 h) under Pb pollution using isobaric tags for relative and absolute quantification technology. A total of 252, 72 and 116 proteins were differentially expressed between M12 (after 12-h Pb treatment) and CK (water-mocked treatment), M24 (after 24-h Pb treatment) and CK, and M48 (after 48-h Pb treatment) and CK, respectively. In addition, 14 differentially expressed proteins were common within each comparison group. Moreover, Cluster of Orthologous Groups enrichment analysis revealed predominance of the proteins involved in posttranslational modification, protein turnover, and chaperones. Additionally, the changes in protein profiles showed a lower concordance with corresponding alterations in transcript levels, indicating important roles for transcriptional and posttranscriptional regulation in the response of maize roots to Pb pollution. Furthermore, enriched functional categories between the successive comparisons showed that the proteins in functional categories of stress, redox, signaling, and transport were highly up-regulated, while those in the functional categories of nucleotide metabolism, amino acid metabolism, RNA, and protein metabolism were down-regulated. This information will help in furthering our understanding of the detailed mechanisms of plant responses to heavy metal stress by combining protein and mRNA profiles.

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Section: Introductionmentioning

confidence: 99%

Proteomic changes in maize as a response to heavy metal (lead) stress revealed by iTRAQ quantitative proteomics

Gao

Peng

et al. 2016

Genet. Mol. Res.

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“…It is also difficult to evaluate the effects of various factors such as light, temperature, soil pH, and rhizosphere microorganisms in different batch experiments [21,22]. Hairy roots, which possess all the morphological and physiological characteristics of normal roots and allow indefinite propagation, have been proven as a convenient experimental tool for investigating the interactions between plant cells and metal ions [22][23][24][25]. The hairy roots of Thlaspi caerulescens were chosen to study oxidative stress and the response of the antioxidant defense system under Cd stress [26].…”

Section: Introductionmentioning

confidence: 99%

“…Three basic helix-loop-helix transcription factors (FER-like Deficiency Induced Transcripition Factor (FIT), AtbHLH38, and AtbHLH39) of Arabidopsis were reported to be involved in the plant's response to Cd stress, and the tolerance of transgenic Arabidopsis to Cd was significantly improved [33]. Moreover, an RNA-Seq method, which can be used to analyze gene expression, has been successfully developed to further investigate the molecular mechanism of response to various biotic and abiotic stresses in various plants [8,24,[34][35][36][37]. Data on transcriptome differences indicate that the difference in the iron-deficient transporter gene in response to Cd stress was found to be related to Cd uptake and redistribution in both Solanum nigrum L. and Solanum torvum L. [38].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress

Sun

Cao

et al. 2019

IJMS

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Brassica campestris L., a hyperaccumulator of cadmium (Cd), is considered a candidate plant for efficient phytoremediation. The hairy roots of Brassica campestris L are chosen here as a model plant system to investigate the response mechanism of Brassica campestris L. to Cd stress. High-throughput sequencing technology is used to identify genes related to Cd tolerance. A total of 2394 differentially expressed genes (DEGs) are identified by RNA-Seq analysis, among which 1564 genes are up-regulated, and 830 genes are down-regulated. Data from the gene ontology (GO) analysis indicate that DEGs are mainly involved in metabolic processes. Glutathione metabolism, in which glutathione synthetase and glutathione S-transferase are closely related to Cd stress, is identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A Western blot shows that glutathione synthetase and glutathione S-transferase are involved in Cd tolerance. These results provide a preliminary understanding of the Cd tolerance mechanism of Brassica campestris L. and are, hence, of particular importance to the future development of an efficient phytoremediation process based on hairy root cultures, genetic modification, and the subsequent regeneration of the whole plant.

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“…Wheat consumption is much higher in northern than in southern China. Metal(loid)s that have accumulated in the body over a long period can lead to chronic diseases and cancer, with As, Cr, and Cd being considered as carcinogens [17][18][19][20][21][22][23][24].Many studies have reported that basic soil characteristics, including pH, organic matter, cation exchange capacity (CeC), and soil clay content, affect the absorption of metal(loid)s by wheat and corn crops. However, most of these studies were conducted within carefully controlled environments using potted plants or field plots, and focused on relevant variables in specific fields [20,[25][26][27][28][29][30].…”

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confidence: 99%

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confidence: 99%

Bioconcentration, Potential Health Risks, and a Receptor Prediction Model of Metal(loid)s in a Particular Agro-Ecological Area

Cai

Song

2019

Applied Sciences

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To investigate the bioconcentration and potential health risks of metal(loid)s in a particular agro-ecological area, 230 pairs of soil and corresponding crop grain samples were collected from typical corn and wheat plants. The geo-accumulation index (Igeo), bioconcentration factors (BCF), health risk assessment (the target hazard quotient), and Receptor Prediction Model (PCS-SMLR) analysis were adopted to study the spatial distribution, assess the health risks, and predict the relationship between metal(loid)s and soil properties. It was found that the mean concentrations of Cu, Zn, Pb, Hg, and Cd in the study area’s agricultural soils exceeded the background soil concentrations, especially for Cd (0.2 mg/kg), Meanwhile, the corresponding Cd concentration in wheat samples was higher than the food quality limit. The results of the Igeo showed that the samples with a value higher than 0 for Cd and Hg accounted for 47.83% and 33.48%, respectively. The results of BCF of Cu, Zn, Cd, and As were higher in wheat than in corn, except for Ni. The target hazard quotient (TTHQ) of health risk of wheat, corn, and soil were higher for children (2.48) than adults (1.78), showing a potential health risk for individuals who mostly consume wheat. In addition, the PCS-SMLR analysis of the BCF prediction model for Cu, Zn, As, and soil properties showed differences in terms of the influences from wheat and corn. These results provide valuable information that not only can help local residents improve the staple food structure, but also can get provide a reference metal(loid)s concentration level for agricultural soils in the study area and restore a sustainable agro-ecological environment.

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The development dynamics of the maize root transcriptome responsive to heavy metal Pb pollution

Cited by 23 publications

References 28 publications

Proteomic changes in maize as a response to heavy metal (lead) stress revealed by iTRAQ quantitative proteomics

Proteomic changes in maize as a response to heavy metal (lead) stress revealed by iTRAQ quantitative proteomics

Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress

Bioconcentration, Potential Health Risks, and a Receptor Prediction Model of Metal(loid)s in a Particular Agro-Ecological Area

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