The development and validation of a solid phase extraction/HPLC method for the determination of aflatoxins in groundnut meal

Roch, Olive G.; Blunden, Gerald; Coker, R. D.; Nawaz, S.

doi:10.1007/bf02276184

Cited by 13 publications

(4 citation statements)

References 6 publications

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“…TFA derivatization was companied by considerable disadvantages. These included peak broadening caused by residual TFA and instability of the derivatization products, aflatoxin B 2 a and G 2 a in methanol [205,208,213]. Additionally, TFA derivatization had to be conducted offline prior to LC separation, making it less desirable compared to post-column derivatization, which could be integrated with LC-FLD as an online operation.…”

Section: Liquid Chromatographymentioning

confidence: 99%

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Zhang

Banerjee

2020

Toxins

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As a class of mycotoxins with regulatory and public health significance, aflatoxins (e.g., aflatoxin B1, B2, G1 and G2) have attracted unparalleled attention from government, academia and industry due to their chronic and acute toxicity. Aflatoxins are secondary metabolites of various Aspergillus species, which are ubiquitous in the environment and can grow on a variety of crops whereby accumulation is impacted by climate influences. Consumption of foods and feeds contaminated by aflatoxins are hazardous to human and animal health, hence the detection and quantification of aflatoxins in foods and feeds is a priority from the viewpoint of food safety. Since the first purification and identification of aflatoxins from feeds in the 1960s, there have been continuous efforts to develop sensitive and rapid methods for the determination of aflatoxins. This review aims to provide a comprehensive overview on advances in aflatoxins analysis and highlights the importance of sample pretreatments, homogenization and various cleanup strategies used in the determination of aflatoxins. The use of liquid-liquid extraction (LLE), supercritical fluid extraction (SFE), solid phase extraction (SPE) and immunoaffinity column clean-up (IAC) and dilute and shoot for enhancing extraction efficiency and clean-up are discussed. Furthermore, the analytical techniques such as gas chromatography (GC), liquid chromatography (LC), mass spectrometry (MS), capillary electrophoresis (CE) and thin-layer chromatography (TLC) are compared in terms of identification, quantitation and throughput. Lastly, with the emergence of new techniques, the review culminates with prospects of promising technologies for aflatoxin analysis in the foreseeable future.

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Section: Liquid Chromatographymentioning

confidence: 99%

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Zhang

Banerjee

2020

Toxins

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“…In Fig. 2 Sometimes the TFA has to be evaporated after the reaction, before the injection solution is added, the avoid excessive peak broadening on the first part of the column [42]. The reproducibility for the derivatized B 1 and G 1 compounds is comparable to that for B, and G,, which indicates that the derivatization procedure does not introduce significant error.…”

Section: Precolumn Hydrolysis Of B and Gmentioning

confidence: 92%

Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection

Kok

1994

Journal of Chromatography B: Biomedical Sciences and Applicatio

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Various derivatization methods for the fluorometric detection of aflatoxins after separation by HPLC are reviewed. In normal-phase chromatography the sensitivity for aflatoxins B, and B, was improved by using special mobile phases or a flow cell packed with silica-gel particles. In the nowadays more popular reversed-phase methods, the fluorescence intensity of B, and G, can be increased by precolumn derivatization with trifluoroacetic acid or by postcolumn derivatization with iodine or bromine. Optimum conditions for the reactions are discussed. In terms of sensitivity, the three derivatization schemes give similar results. The methods are compared with respect to experimental convenience, selectivity, reproducibility and suitability for automation.

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“…[15] Trifluoroacetic acid (TFA) converts the double bound of the dihydrofuran into hemyacetal derivatives, which do not undergo fluorescence quenching in aqueous solution. [16] Also post column derivatization techniques such as iodination and bromination have been applied successfully, but the need for daily preparation of the iodine solution, the required use of an auxiliary pump, and the time-consuming mobile phase preparation are drawbacks of these techniques. [17] The photochemical reactor for enhance detection (PHRED) system is one of the major objectives proposed.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Cereals Using a Validated Rp-HPLC Method and Phred Derivatization System

Rahmani

Selamat

Khatib

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid-liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31-104.1% for cereal samples spiked at 6.5, 10, and 100 ng=g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45-11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004-0.012, 0.05, and 0.5 ng=g, respectively; whereas. limits of quantification (LOQ) were 0.015-0.05, 0.2, and 2 ng=g, correspondingly.

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The development and validation of a solid phase extraction/HPLC method for the determination of aflatoxins in groundnut meal

Cited by 13 publications

References 6 publications

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection

Simultaneous Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Cereals Using a Validated Rp-HPLC Method and Phred Derivatization System

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