The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species

Davis, William C.; Marušić, Suzana; Lewin, Harris A.; Splitter, Gary A.; Perryman, Lance E.; McGuire, Travis C.; Gorham, J. R.

doi:10.1016/0165-2427(87)90005-5

Cited by 261 publications

(90 citation statements)

References 79 publications

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“…Our staining for CD18, IL-2R, and TCR were consistent with our previous studies (16, 24) (E. Wilson, unpublished observations). MHC I has been previously shown to be homogeneously expressed on bovine lymphocytes (30). These results demonstrate that SAGE is a reliable predictor of gene regulation in this system, particularly for differences in SAGE that are Ն5-fold.…”

Section: High Speed Cell Sorting Of ␥␦ T Cell Subsets and Sage Librarmentioning

confidence: 58%

“…Briefly, a biotinylated oligo(dT) primer (biotin-5Ј-AAGCAGTGGTAACAACGCAGAGTAC(T) 30 VN-3Ј, where V ϭ A, C, or G, and N ϭ T, C, G, or A) and Superscript II reverse transcriptase (Invitrogen) were used to make first-strand cDNA from 100 ng of total RNA. Second-strand synthesis and cDNA amplification were completed by PCR using Advantage2 Polymerase Mix (Clontech, Palo Alto CA) and a switching primer (5Ј-AAGCAGTGGTAACAACGCAGAGTACGCGGG-3Ј) in combination with the original biotinylated oligo(dT).…”

Section: Sage Library Construction and Analysismentioning

confidence: 99%

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Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Meissner

Radke

Hedges

et al. 2003

The Journal of Immunology

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Gene expression profiles were compared in circulating bovine GD3.5+ (CD8−) and GD3.5− (predominantly CD8+) γδ T cells using serial analysis of gene expression (SAGE). Approximately 20,000 SAGE tags were generated from each library. A comparison of the two libraries demonstrated 297 and 173 tags representing genes with 5-fold differential expression in GD3.5+ and GD3.5− γδ T cells, respectively. Consistent with their localization into sites of inflammation, GD3.5+ γδ T cells appeared transcriptionally and translationally more active than GD3.5− γδ cells. GD3.5− γδ T cells demonstrated higher expression of the cell proliferation inhibitor BAP 37, which was associated with their less activated gene expression phenotype. The immune regulatory and apoptosis-inducing molecule, galectin-1, was identified as a highly abundant molecule and was higher in GD3.5+γδ T cells. Surface molecules attributed to myeloid cells, such as CD14, CD68, and scavenger receptor-1, were identified in both populations. Furthermore, expression of B lymphocyte-induced maturation protein, a master regulator of B cell and myeloid cell differentiation, was identified by SAGE analysis and was confirmed at the RNA level to be selectively expressed in γδ T cells vs αβ T cells. These results provide new insights into the inherent differences between circulating γδ T cell subsets.

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Section: High Speed Cell Sorting Of ␥␦ T Cell Subsets and Sage Librarmentioning

confidence: 58%

Section: Sage Library Construction and Analysismentioning

confidence: 99%

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Meissner

Radke

Hedges

et al. 2003

The Journal of Immunology

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“…The hybridoma designation for each antibody follows the cell type the antibody was used to detect (Bo indicates bovine): BoCD4, CACT83B; BoCD8, CACT80C; BoCD2, CH128A; BoCD6, BAQ82A; B-cells, BAQ44A; monocytes, DH59B; null (non-T, non-B, non-monocyte) cells, BAQ4A (Davis et al, 1987(Davis et al, , 1988(Davis et al, , 1991Larsen et al, 1990). Murine monoclonal antibodies against non-lymphocyte antigens were used as isotype controls.…”

Section: Methodsmentioning

confidence: 99%

Effect of bovine immunodeficiency-like virus infection on immune function in experimentally infected cattle

Flaming

Maaten

Whetstone

et al. 1993

Veterinary Immunology and Immunopathology

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Bovine immunodeficiency-like virus (BIV) is a bovine lentivirus that has antigenic and genetic homology with the human immunodeficiency virus. Little work has been reported on the effect of BIV infection on bovine immune function. This study was designed to evaluate lymphocyte blastogenesis, mononuclear cell subset numbers, neutrophil function, hematology, and clinical signs in three groups of cattle. These groups were evaluated at 0-2 months post inoculation (PI, Group 1), 4-5 months PI (Group 2), or 19-27 months PI (Group 3). BIV infected animals were inoculated with the R-29 isolate of BIV in tissue culture cells, peripheral blood mononuclear cells from a R-29 infected calf, or a molecular clone of the R-29 isolate. Most inoculated animals seroconverted to BIV by Western immunoblot. BIV was reisolated from most of the animals inoculated. BIV infection was associated with an increase in the lymphocyte blastogenic response to the mitogen phytohemagglutinin in Groups 2 and 3. Neutrophil antibody dependent cell mediated cytotoxicity and neutrophil iodination were decreased (P<0.05) in BIV infected cattle (Groups 2 and 3 and Group 3, respectively). All animals were clinically normal during the evaluation periods. Notable differences were not observed in the other assessments performed. Work with additional BIV isolates and over longer time frames is warranted. Veterinary Immunology and Immunopathology, 36 ( 1993)

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“…II) were then applied for 18 hours at 4 °C. Monoclonal antibodies (mAb) to CD4, CD8 and B-B4 showed cross-reactivity with these ovine leukocyte antigens [11]. The anti-CD3 polyclonal antibody (pAb) was used as a pan-T cell marker, and was shown to cross-react with ovine T lymphocytes [26].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Pérez¹,

Ortega²,

Bravo³

et al. 2005

Vet. Res.

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-The phenotype of the hepatic inflammatory infiltrate and hepatic lymph nodes (HLN) was analysed in lambs primarily and challenge infected with Fasciola hepatica. Group 1 was primarily and challenge infected with two doses of 200 metacercariae (mc) each and was nontreated. Trickle infection was administered to five groups: group 2 was challenge infected and nontreated; group 3 was primarily infected and non-treated; group 4 was primarily infected and treated with triclabendazole (TCBZ) at 12 weeks postinfection (wpi); group 5 was treated at 4 wpi and challenge infected and group 6 was treated at 12 wpi and challenge infected. An uninfected group was used as the control. The distribution of T cell subpopulations (CD3, CD4 and CD8), and B cells (CD79α, IgM, IgG) was analysed. The hepatic inflammatory infiltrate was represented mainly by CD3 and CD4 T cells, and B cells (CD79α, IgG). These infiltrates were more severe (P < 0.05) in primarily (group 3) or challenge (groups 2, 5 and 6) trickle infected lambs than in the group single challenge infected (group 1). Cellular changes in HLN consisted in an increase of CD4 over CD8 T cells and an increase of B cells and IgG + plasma cells, and they were more severe in primarily and challenge trickle infected groups than in the group infected with two larger doses of mc, although significant differences were not found with respect to all challenge trickle infected groups. The strong local cellular and humoral immune responses did not protect against subsequent infections, neither in non-treated lambs (group 2) nor in lambs treated with TCBZ at 4 wpi (group 5) or 12 wpi (group 6).

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The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species

Cited by 261 publications

References 79 publications

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Effect of bovine immunodeficiency-like virus infection on immune function in experimentally infected cattle

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

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