Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected 
with <i>Fasciola hepatica,</i> with and without triclabendazole treatment

Pérez, J.; Ortega, J.; Bravo, Ana; Díez‐Baños, P.; Morrondo, P.; Moreno, T.; Martı́nez-Moreno, A.

doi:10.1051/vetres:2004047

Cited by 20 publications

(8 citation statements)

References 26 publications

(41 reference statements)

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“…Using this marker, it was established that the proportion of CD4 + T cells in the local lymph nodes of ITT sheep did not alter significantly in response to Fasciola infection. This is in agreement with previous studies showing that the progressive increase in HLN weight and cell number after Fasciola infection was accompanied by a disproportionate increase in B cells (33,34). The contrasting cytokine profiles between F. hepatica ‐ (susceptible) and F. gigantica‐ (resistant) infected ITT sheep at 3 weeks p.i.…”

Section: Discussionsupporting

confidence: 94%

Resistance to liver fluke infection in the natural sheep host is correlated with a type‐1 cytokine response

Pleasance

Wiedosari

Raadsma

et al. 2011

Parasite Immunology

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Indonesian thin-tail (ITT) sheep can resist infection with Fasciola gigantica but not F. hepatica and presents an ideal model to investigate the mechanisms of liver fluke resistance in a natural host. This study examines the local and systemic immune responses of sheep during Fasciola infection and demonstrates that different anatomical tissues display distinct cytokine profiles consistent with liver fluke migration. The study also reveals a significant difference in the cytokine and antibody profiles of ITT sheep infected with F. gigantica compared with F. hepatica, with a higher ratio of IL-4/IFN-γ mRNA expression and specific IgG1/IgG2 antibodies strongly correlating with pathology. Interestingly, the significant type-1 cytokine profile occurred in the lymph node closest to the site of infection at a time when the effective immune response against F. gigantica liver flukes is thought to occur. When the same F. gigantica infection in the resistant ITT sheep was compared with the susceptible Merino breed, the resistant type-1 phenotype against liver fluke infection was only observed in the ITT sheep. These studies provide the first evidence to suggest that the induction of an early type-1 immune response in this natural sheep host may be responsible for the ability to resist liver fluke infection.

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Section: Discussionsupporting

confidence: 94%

Resistance to liver fluke infection in the natural sheep host is correlated with a type‐1 cytokine response

Pleasance

Wiedosari

Raadsma

et al. 2011

Parasite Immunology

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“…Fasciolosis control is almost exclusively carried out by strategic applications of a wide number of effective anthelmintics (Sanchez-Andrade et al 2001). Only triclabendazole is efficacious against both pre-adults in hepatic parenchyma and adults in the bile ducts (Boray et al 1983;Perez et al 2005). However, the effectiveness of triclabendazole represents problems of resistance (Fairweather and Boray 1999;Robinson et al 2002;Fairweather 2005;Fairweather 2009).…”

Section: Introductionmentioning

confidence: 99%

Ovicidal effect of the methanolic extract of ginger (Zingiber officinale) on Fasciola hepatica eggs: an in vitro study

Moazeni

Khademolhoseini

2014

J Parasit Dis

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Fasciolosis is of considerable economic and public health importance worldwide. Little information is available on the ovicidal effects of anthelminthic drugs. The use of ovicidal anthelmintics can be effective in disease control. In this study, the effectiveness of the methanolic extract of ginger (Zingiber officinale) on the eggs of Fasciola hepatica is investigated. Fasciola hepatica eggs were obtained from the gall bladders of naturally infected sheep and kept at 4 °C until use. The eggs were exposed to varying concentrations of ginger extract (1, 5, 10, 25 and 50 mg/mL) for 24, 48 and 72 h. To investigate the effect of the ginger extracts on the miracidial formation, the treated eggs were incubated at 28 °C for 14 days. The results indicated that F. hepatica eggs are susceptible to the methanolic extract of Z. officinale. The ovicidal effect of ginger extract at a concentration of 1 mg/mL with 24, 48 and 72 h treatment time was 46.08, 51.53 and 69.09 % respectively (compared with 22.70 % for control group). The ovicidal effect of ginger extract at a concentration of 5 mg/mL after 24 h was 98.84 %. One hundred percent ovicidal efficacy was obtained through application of ginger extract at concentrations of 5 and 10 mg/mL with a 48 and 24 h treatment time respectively. The in vitro ovicidal effect of the methanolic extract of Z. officinale was satisfactory in this study, however, in vivo efficacy of this extract, remains for further investigation. To the best of our knowledge, this is the first report on the ovicidal effect of Z. officinale against F. hepatica eggs.

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“…Cornelissen et al (2001), were able to detect positive animals by egg count only from week 9 in sheep and even later in cattle from week 15 onwards after natural infection of animals with F. hepatica, and Arias et al (2005), in their experimental work have detected positive animals through fecal examination 11 weeks after primary infection. Perez et al (2005), reported that eggs in stools indicates the presence of mature adult flukes in the bile ducts and gallbladder, but a period longer than 10 weeks after infection is required so most of the pathological damage has already occurred. The classification of liver pathology and the level of Fasciola infestation within the herd were compared based on postmortem findings of liver lesions and antibody detection by ELISA, the results of light and sever infection were similar, 23% and 2% of the animals were lightly and severely infected respectively, in both tests.…”

Section: Discussionmentioning

confidence: 99%

Comparative diagnostic techniques in ruminant Fasciolosis: fecal sedimentation, indirect ELISA, liver inspection and serum enzyme activities

Adamu¹,

Wossene²,

Tilahun³

et al. 2019

Ethiop. Vet. J.

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Different diagnostic tests have some disadvantages in diagnosing Fasciolosis. A cross-sectional study was conducted to estimate the prevalence and to compare the different diagnostic techniques in ruminant Fasciolosis: ELISA, biochemical analysis, liver and coprological examination. Out of the 324 cattle, 350 sheep and 385 goats slaughtered at Bishoftu, ELFORA Export Abattoir, 125 (38.5%), 4 (1.1%) and 2 (0.5%) and 108 (33%) 4 (1.1%) and 2 (0.5%) were

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Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Cited by 20 publications

References 26 publications

Resistance to liver fluke infection in the natural sheep host is correlated with a type‐1 cytokine response

Resistance to liver fluke infection in the natural sheep host is correlated with a type‐1 cytokine response

Ovicidal effect of the methanolic extract of ginger (Zingiber officinale) on Fasciola hepatica eggs: an in vitro study

Comparative diagnostic techniques in ruminant Fasciolosis: fecal sedimentation, indirect ELISA, liver inspection and serum enzyme activities

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