The deubiquitinating enzyme MoUbp8 is required for infection-related development, pathogenicity, and carbon catabolite repression in Magnaporthe oryzae

Yang, Jie; Chen, Dongjie; Matar, Khalid Abdelkarim Omer; Zheng, Tianhong; Zhao, Qianqian; Xie, Yuman; Gao, Xiuqin; Li, Mingyang; Wang, Baohua; Lu, Guodong

doi:10.1007/s00253-020-10572-5

Cited by 17 publications

(35 citation statements)

References 62 publications

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“…This indicates that the deletion of the gene SsCI33130 will cause a significant decrease in the pathogenicity of S. scitamineum, confirming that deubiquitinase is involved in the pathogenicity of the fungus, which is consistent with previous findings (Enyenihi and Saunders, 2003;Wei et al, 2012;Yang et al, 2020). In addition, we found that the deletion of this gene did not affect the growth, morphology, abiotic stress, or other phenotypes of the haploid spores of S. scitamineum, which contrasts with the results reported by Wei et al (2012) and Yang et al (2020). The main reason may be that the functions of deubiquitinases in different families may differ.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, the incidence rate of sugarcane smut in the combinations with SsCI33130 knockout mutants ( Ss33130 + + Ss33130 − , Ss16 + + Ss33130 − , and Ss16 − + Ss33130 + ) was significantly lower than that in the combinations without knockout mutants (Ss16 + + Ss16 − , COM33130 + + COM33130 − , Ss16 + + COM33130 − , and Ss16 − + COM33130 + ). This indicates that the deletion of the gene SsCI33130 will cause a significant decrease in the pathogenicity of S. scitamineum, confirming that deubiquitinase is involved in the pathogenicity of the fungus, which is consistent with previous findings (Enyenihi and Saunders, 2003;Wei et al, 2012;Yang et al, 2020). In addition, we found that the deletion of this gene did not affect the growth, morphology, abiotic stress, or other phenotypes of the haploid spores of S. scitamineum, which contrasts with the results reported by Wei et al (2012) and Yang et al (2020).…”

Section: Discussionsupporting

confidence: 87%

“…The main reason may be that the functions of deubiquitinases in different families may differ. The results reported by Wei et al (2012) and Yang et al (2020) were mainly based on the deubiquitinases in the UBP/USP family, while the present study focused on the OTU1-deubiquitinases of the OTU family. Sebastian and Stefan (2006) found that the phenotype of the deletion mutant of the otu1 gene encoding OTU1-deubiquitinase in yeast was not significantly different from that of the wild type, which was consistent with the fact that the deletion of the SsCI33130 gene in this study did not cause phenotypic changes in the growth, morphology, and abiotic stress of S. scitamineum.…”

Section: Discussionmentioning

confidence: 78%

“…Protein DUB plays an important role in the vegetative growth, melanin synthesis, sporulation, colonization, and pathogenicity of fungi (Enyenihi and Saunders, 2003;Wei et al, 2012;Qin et al, 2021). The deletion of genes encoding deubiquitinase will lead to defects in fungal vegetative growth and melanin synthesis, as well as decreases in conidia production, colonization ability, and pathogenicity (Yang et al, 2020). In this study, the incidence rate of sugarcane smut in the combinations with SsCI33130 knockout mutants ( Ss33130 + + Ss33130 − , Ss16 + + Ss33130 − , and Ss16 − + Ss33130 + ) was significantly lower than that in the combinations without knockout mutants (Ss16 + + Ss16 − , COM33130 + + COM33130 − , Ss16 + + COM33130 − , and Ss16 − + COM33130 + ).…”

Section: Discussionmentioning

confidence: 99%

“…The deletion of the gene Moubp8 encoding UBP-deubiquitinase in Magnaporthe oryzae led to a deficiency in the vegetative growth and melanin synthesis of the pathogen, resulting in a significant decrease in conidia production and pathogenicity. In addition, the colonization ability of the mutant was significantly decreased by an osmotic colonization test (Yang et al, 2020). In Saccharomyces cerevisiae, the deletion of the gene UBP14 encoding deubiquitinase results in a reduction of meiosis efficiency in S. cerevisiae, and the mutation of the DOA4 gene encoding deubiquitinase affects the sporulation ability of the fungus (Swaminathan et al, 1999;Enyenihi and Saunders, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Ovarian Tumor Domain-Containing Proteases-Deubiquitylation Enzyme Gene SsCI33130 Involved in the Regulation of Mating/Filamentation and Pathogenicity in Sporisorium scitamineum

Cai

Deng

et al. 2021

Front. Microbiol.

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Sugarcane is an important sugar crop. Sugarcane smut, caused by Sporisorium scitamineum, is a worldwide sugarcane disease with serious economic losses and lack of effective control measures. Revealing the molecular pathogenesis of S. scitamineum is very helpful to the development of effective prevention and control technology. Deubiquitinase removes ubiquitin molecules from their binding substrates and participates in a variety of physiological activities in eukaryotes. Based on the transcriptome sequencing data of two isolates (Ss16 and Ss47) of S. scitamineum with different pathogenicities, SsCI33130, a gene encoding an OTU1-deubiquitin enzyme, was identified. The positive knockout mutants and complementary mutants of the SsCI33130 gene were successfully obtained through polyethylene glycol-mediated protoplast transformation technology. In order to study the possible function of this gene in pathogenicity, phenotypic comparison of the growth, morphology, abiotic stress, sexual mating, pathogenicity, and gene expression levels of the knockout mutants, complementary mutants, and their wild type strains were conducted. The results demonstrated that the gene had almost no effect on abiotic stress, cell wall integrity, growth, and morphology, but was related to the sexual mating and pathogenicity of S. scitamineum. The sexual mating ability and pathogenicity between the knockout mutants or between the knockout mutant and wild type were more significantly reduced than between the wild types, the complementary mutants, or the wild types and complementary mutants. The sexual mating between the knockout mutants or between the knockout mutant and wild type could be restored by the exogenous addition of small-molecule signaling substances such as 5 mM cyclic adenosine monophosphate (cAMP) or 0.02 mM tryptophol. In addition, during sexual mating, the expression levels of tryptophol and cAMP synthesis-related genes in the knockout mutant combinations were significantly lower than those in the wild type combinations, while the expression levels in the complementary mutant combinations were restored to the level of the wild type. It is speculated that the SsCI33130 gene may be involved in the development of sexual mating and pathogenicity in S. scitamineum by regulating the synthesis of the small-molecule signaling substances (cAMP or tryptophol) required during the sexual mating of S. scitamineum, thereby providing a molecular basis for the study of the pathogenic mechanisms of S. scitamineum.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ovarian Tumor Domain-Containing Proteases-Deubiquitylation Enzyme Gene SsCI33130 Involved in the Regulation of Mating/Filamentation and Pathogenicity in Sporisorium scitamineum

Cai

Deng

et al. 2021

Front. Microbiol.

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The role of MrUbp4, a deubiquitinase, in conidial yield, thermotolerance, and virulence in Metarhizium robertsii

Zhang,

Chen,

Zhang

et al. 2024

Journal of Invertebrate Pathology

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Degradation of α-Subunits, Doa1 and Doa4, are Critical for Growth, Development, Programmed Cell Death Events, Stress Responses, and Pathogenicity in the Watermelon Fusarium Wilt Fungus Fusarium oxysporum f. sp. niveum

Noman

Azizullah

Gao

et al. 2023

J. Agric. Food Chem.

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The ubiquitin−proteasome system (UPS) regulates protein quality or control and plays essential roles in several biological and biochemical processes in fungi. Here, we present the characterization of two UPS components, FonDoa1 and FonDoa4, in watermelon Fusarium wilt fungus, Fusarium oxysporum f. sp. niveum (Fon), and their biological functions. FonDoa1 localizes in both the nucleus and cytoplasm, while FonDoa4 is predominantly present in the cytoplasm. Both genes show higher expression in germinating macroconidia at 12 h. Deletion of FonDoa1 or FonDoa4 affects vegetative growth, conidiation, conidial germination/morphology, apoptosis, and responses to environmental stressors. FonDoa1, but not FonDoa4, positively regulates autophagy. The targeted disruption mutants exhibit significantly attenuated pathogenicity on watermelon due to defects in the infection process and invasive fungal growth. Further results indicate that the WD40, PFU, and PUL domains are essential for the function of FonDoa1 in Fon pathogenicity and environmental stress responses. These findings demonstrate the previously uncharacterized biological functions of FonDoa1 and FonDoa4 in phytopathogenic fungi, providing potential targets for developing strategies to control watermelon Fusarium wilt.

show abstract

The deubiquitinating enzyme MoUbp8 is required for infection-related development, pathogenicity, and carbon catabolite repression in Magnaporthe oryzae

Cited by 17 publications

References 62 publications

Ovarian Tumor Domain-Containing Proteases-Deubiquitylation Enzyme Gene SsCI33130 Involved in the Regulation of Mating/Filamentation and Pathogenicity in Sporisorium scitamineum

Ovarian Tumor Domain-Containing Proteases-Deubiquitylation Enzyme Gene SsCI33130 Involved in the Regulation of Mating/Filamentation and Pathogenicity in Sporisorium scitamineum

The role of MrUbp4, a deubiquitinase, in conidial yield, thermotolerance, and virulence in Metarhizium robertsii

Degradation of α-Subunits, Doa1 and Doa4, are Critical for Growth, Development, Programmed Cell Death Events, Stress Responses, and Pathogenicity in the Watermelon Fusarium Wilt Fungus Fusarium oxysporum f. sp. niveum

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