The Determination of Serum Protein-Bound Iodine by Alkali Incineration Values in Normal Subjects

The estimation of serum protein-bound iodine by alkaline incineration

“…Table 5 shows the reports of some other workers on the reliability of their methods. Results comparable to those reported here are claimed by Sobel & Sapsin (1952), using both their own and Chaney's (1940) acid-distillation methods, by Skanse & Hedenskog (1955), using a modification of the Barker et al (1951) method, and by Thompson et al (1956), using another modification of the same method. However, Chaney's (1940) method requires 3 ml.…”

“…These authors use the highly acid conditions which have been criticized above. Skanse & Hedenskog (1955) also use an acid solution to elute iodide from ashed protein, which probably accounts for their observing a 15 % loss of iodine during the ashing process. They allow for this loss in their calibration of the reaction but, (Itg.…”

“…The zinc hydroxide precipitates were allowed to stand for 3 hr. before centrifuging, as recommended by Skanse & Hedenskog (1955), although no experiments were carried out to confirm the value of this procedure. For these determinations, the ashing temperature was not allowed to rise above 6100.…”