The estimation of serum protein-bound iodine by alkaline incineration

Acland, J. D.

doi:10.1042/bj0660177

Cited by 94 publications

(24 citation statements)

References 22 publications

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“…Blood was taken at this time for analysis of serum Protein Bound Iodine (PBI) carried out by the method of Acland (1957), the results of which are given in Table 1. There is a highly significant reduction (P< 0-001) in PBI in spite of the presence of residual thyroid tissue in four ewes. Subsequently, the ewes were observed until lambing was completed, and a record was made of abortions, stillbirths and lamb viability ; in one thyroidectomized ewe that died during lambing the regenerated thyroid tissue was removed and weighed.…”

Section: Methods and Resultsmentioning

confidence: 99%

The Effect of Thyroid Deficiency on the Oestrous Cycle and Conception in the Ewe

Falconer¹

1963

Journal of Endocrinology

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One half of a flock of twenty-four 3-to 4-year-old Merino ewes were thyroidectomized and the other half subjected to a sham-operation, oestrous cycles being recorded before and after surgery in both groups. A year later a record was again kept of oestrous cycles, and in both series of observations there were no apparent changes due to thyroidectomy.An intact ram was run with the flock on two occasions, at 3 and 14 months, after thyroidectomy. As judged by lambing in the first instance, and dissection in the second, there were no effects of thyroidectomy on conception.It was concluded that thyroidectomy has no apparent influence on the oestrous cycle and conception in the ewe.

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Section: Methods and Resultsmentioning

confidence: 99%

The Effect of Thyroid Deficiency on the Oestrous Cycle and Conception in the Ewe

Falconer¹

1963

Journal of Endocrinology

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“…Serum and skin samples obtained from each fish were stored frozen until analysis. Iodide content was determined by Acland's (1957) modification of the method of Barker et al (1951). Following the procedure of Grossman and Grossman (1955) brucine sulfate was added to terminate the reaction and stabilize the residual ceric color.…”

Section: Methodsmentioning

confidence: 99%

Seasonal changes in the extrathyroidal distribution of iodide in the teleost fish the burbot, Lota lota L.

Wiggs¹

1971

Can. J. Zool.

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WIGGS, A. J. 1971. Seasonal changes in the extrathyroidal distribution of iodide in the teleost fish the burbot, Lota lota L. Can. J. Zool. 49: 1505-1511. The distribution of radioactivity in tissues of adult burbot weighing 1 to 3 kg was d e t e w e d by measuring the T / S ratio (tissue radioactivity/serum radioaclivlty) at intervals after the inject~on of radioiodide. In one instance the skin which comprises 12% of body weight of the burbot showed a TIS ratio of 29.3 and accounted for up to 27% of the injected dose of radioiodide. By comparison, in another study, the thyroid showed a T/S ratio of 5.2, representing 2.2% of the injected dose at 9.5C. The radioactivity (T/S ratio) in the skin increased until 30 h afier injection and then declined.Non-radioactive iodide values of skin and serum wcre detennincd on a na~ural populalion o~t the course of a year. Values for skin iodide ranged front 227 pg 11 00 g to 6 16 pg I00 g wh~fe serum ~odide values ranged from 7 pg/100 rnl to 30 pg/100 ml. These values also indicate the magnitude of seasonal variation, which, in the case of serum iodide concentration, parallels the seasonal changes in lake temperature.La distribution de la radioactivitk dans les tissus de la lote adulte pesant de 1 h 3 kg a kt6 dttermink en mesurant la rapport T/S (radioactivitk du tissu/mdioactiv~te du strum) A certains intervalles a p r e I'injection de radioiodure. Dans un cas, la peau qui represente 12% du poids d o corps de la lote rnonlralt un rapport T/S de 29.3 et contenait plus de 27% de In dose inject& de radioiodure. En cornparaison. dans une autre ktude, la thyro?de montrait un rapport 7 1 S de 5.2, representant 2.2y0 de !a dose inject& A 9.5C. La radioactivitk (rapport T/S) augmentait dans la peau jusqu'8 30 h apks l'inject~on et elle dimmuait ensuite.Les teneurs en iodure non-radioactif de la peau et du sbum ont kt6 ddterminks dans une population naturelle au cours de l'annk. Les valeurs pour l'iodure de la peau variaient de 227 pg/lO? g .A 816pg/100 g, alors que celles du sQum variaient de 7 pg/100 rnl A 30 pg/100 I$. Ces valeurs indlquent aussi I'amplitude des variations saisonnikres qui, dans le cas de concentration en lodure du serum, etaient parallkles aux changements de la tempkrature dans le lac.

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“…bodyweight; this was found to be more reliable than calculations based on extrapolation of PB125I to zero time in 36 determinations. PBI was determined by the method of Acland (1957) or by perchloric acid digestion (Riley & Gochman, 1964). The rate of thyroxine utilization was calculated as the product of the fractional turnover rate, the distribution volume and PBI (as thyroxine).…”

Section: Methodsmentioning

confidence: 99%

Thyroxine Utilization in Lambs in Natural and Controlled Environments

Falconer¹,

Marchant²

1970

Journal of Endocrinology

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The rate of thyroxine utilization has been measured from the rate of degradation of [125I]thyroxine and the concentration of plasma proteinbound iodine in ewe lambs. Repeated measurements were made between 10 and 60 weeks of age in two groups of lambs, one in conditions including temporarily inadequate nutrition and outdoor ambient temperatures, and the other in a controlled environment with free access to food.The lambs in the controlled environment showed an increase in thyroxine utilization with increasing age throughout the experiment, whereas the 'outdoor lambs' showed a decrease in hormone utilization until after 34 weeks of age. The 'outdoor lambs' also lost bodyweight between the 20th and 26th week of age due to the poor quality of the pasture, and this undernutrition is considered to have caused the lowered rate of thyroxine utilization. After 26 weeks of age the nutrition of these lambs was adequate but thyroxine utilization did not increase until after 34 weeks of age, when the mean environmental temperature was about 5\ s=deg\ .It was concluded that undernutrition caused a marked depression in thyroxine utilization of young lambs.

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The estimation of serum protein-bound iodine by alkaline incineration

Cited by 94 publications

References 22 publications

The Effect of Thyroid Deficiency on the Oestrous Cycle and Conception in the Ewe

The Effect of Thyroid Deficiency on the Oestrous Cycle and Conception in the Ewe

Seasonal changes in the extrathyroidal distribution of iodide in the teleost fish the burbot, Lota lota L.

Thyroxine Utilization in Lambs in Natural and Controlled Environments

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