The determination of ribose nucleic acid in the heart and associated tissues of house flies of various ages

Yale, T.H.; Ballard, Ralph C.

doi:10.1016/0010-406x(66)90543-3

Cited by 8 publications

(2 citation statements)

References 6 publications

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“…A GE-RELATED variations in total nucleic acid levels have been studied in Drosophila melanogaster and several other dipteran species, in some cases with contradictory results. There have been reports of increasing nucleic acid content during the first 5 days of adult life in D. melanogaster and the mosquito Aedes aegypti (1,2), while constant levels throughout adult life were reported for the housefly Musca domestica (3). Other studies report agerelated declines in nucleic acid content in various animals, including D. melanogaster (4)(5)(6)(7)(8)(9).…”

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confidence: 99%

Age-Related RNA Decline in Adult Drosophila melanogaster

Tahoe

Mokhtarzadeh

Curtsinger

2004

The Journals of Gerontology Series A: Biological Sciences and M

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We investigated the correlation between age and total RNA levels in long-lived and control lines of Drosophila melanogaster. Total RNA was extracted at 10 ages from 1-63 days posteclosion from 3 inbred lines, with replication. Three different methods of RNA quantitation gave highly correlated estimates. Total RNA declined substantially with age, exhibiting a dramatic drop in the first few days of adult life. We find no evidence for a causal relationship between adult longevity and total RNA levels, since long-lived and control lines exhibited similar patterns of age-related RNA decline. These observations suggest that the dramatic decline in total RNA that occurs early in adult life does not explain the twofold differences in life span between lines. The pattern of age-specific decline coincides with published observations on age-specific metabolic rates, and suggests that 14-day-old flies are functionally senescent.

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confidence: 99%

Age-Related RNA Decline in Adult Drosophila melanogaster

Tahoe

Mokhtarzadeh

Curtsinger

2004

The Journals of Gerontology Series A: Biological Sciences and M

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“…The quantitative determination of micro-amounts of nucleic acid has attracted a great deal of attention in the fields of medicine and molecular biology. Many methods have been developed, such as direct determination, including ultraviolet absorption and determination of ribose or deoxyribose in nucleic acid, spectrophotometry, chemiluminescence, electrochemical chromatography, including high-performance liquid chromatography and paper chromatography, capillary electrophoresis, and resonance light scattering . However, low sensitivity and easy interruption by protein and other biomolecules existed in these methods in common.…”

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confidence: 99%

Fluorescence Determination of DNA Using the Gatifloxacin−Europium(III) Complex

Wang

Guo

et al. 2011

J. Agric. Food Chem.

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A method for the determination of DNA based on the fluorescence intensity of the gatifloxacin-europium(III) (GFLX-Eu(3+)) complex that could be enhanced by DNA was developed. The GFLX-Eu(3+) complex showed an up to 6-fold enhancement of luminescence intensity after adding DNA. Under the optimized experimental conditions, the system exhibited a linear relationship between the enhanced fluorescence intensity and the concentration of calf thymus DNA (ctDNA) over the range from 1.0 × 10(-8) to 1.5 × 10(-6) g mL(-1), with a correlation coefficient (R) of 0.997, and the detection limit (3σ) of the method was determined as 6.0 × 10(-9) g mL(-1). The mechanism of the fluorescence enhancement effect was also discussed.

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The effect of apholate and thiotepa on nucleic acid synthesis and nucleotide ratios in housefly eggs

Painter

Kilgore

1967

Journal of Insect Physiology

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The determination of ribose nucleic acid in the heart and associated tissues of house flies of various ages

Cited by 8 publications

References 6 publications

Age-Related RNA Decline in Adult Drosophila melanogaster

Age-Related RNA Decline in Adult Drosophila melanogaster

Fluorescence Determination of DNA Using the Gatifloxacin−Europium(III) Complex

The effect of apholate and thiotepa on nucleic acid synthesis and nucleotide ratios in housefly eggs

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