The determination of phenolic compounds in artichoke flower head extracts by HPLC GC/MS technique

Fedosov, A. I.; Kyslychenko, Olexandra; Gudzenko, Andrei; Semenchenko, Olga; Kyslychenko, V. S.

doi:10.14748/ssp.v3i2.1701

Cited by 5 publications

(7 citation statements)

References 8 publications

(12 reference statements)

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“…The fragmentation patterns of those compounds agree with the reported one (Abu-Reidah et al, 2012;Abu-Reidah et al, 2013;Andrei Fedosov et al, 2016;Chen et al, 2012;D'Antuono et al, 2015;Farag et al, 2013;Gebhardt, 1998;Gebhardt and Fausel, 1997;Llorach et al, 2002;Moglia et al, 2008;Sanchez-Rabaneda et al, 2003;Schutz et al, 2006;Sonnante et al, 2010;Wu et al, 2013).…”

Section: Identification Of Bioactive Compounds Isolated From Endophytsupporting

confidence: 87%

In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials

2018

Global NEST: The International Journal

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Plant-associated microorganisms, especially endophytic fungi, represent an untapped resource for the discovery of biologically active natural products. The objectives of this study were to isolate, identify endophytic fungi, and produce their bioactive metabolites from the leaves of two varieties of Egyptian artichoke namely: French Hyrious and Egyptian Baladi. In addition, assess of their total antioxidant capacity (TAC), total phenolic content (TPC) and total flavonoid content (TFC). The results of this novel study show a total of 35 endophytic fungal species belonging to 14 genera were isolated from both artichoke leaves with gross total counts of colonizing endophytic fungi ranged from 71 to 123 cfu which is matching 78.89% to 136.67% of colonization frequency. All taxa recovered were assigned to Ascomycetes. In addition, there is high species richness and diversity indices of endophytic filamentous fungi in the leaves Baladi Artichoke as compared to its French rival.  Alternaria alternata were found to be the most frequently isolated dominant species. The TAC, TPC and TFC of the fungal cultures ranged from 163 to 681 mgAAE/gDW, 10.38 to 40.30 mgGAE/ gDW, and 13.92 to 173.55 mgQE/gDW, respectively. Furthermore, LC-ESI-MS/MS conﬁrmed the presence of 1,3-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid in the methanolic extract of A. alternata. Hence, this novel study suggested that the metabolites produced by endophytic fungi associated with Egyptian artichoke could be explored as an economic and potential natural resources with diverse pharmaceutical and biological activities.

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Section: Identification Of Bioactive Compounds Isolated From Endophytsupporting

confidence: 87%

In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials

2018

Global NEST: The International Journal

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“…A chlorogenic acid compound in the garlic's ethanolic extract was tentatively identified at an m/z of 354 with an ion fragment at an m/z of 191, as revealed in Peak 2. Peak 3, with an m/z of 180 and four ion fragments at m/z ratios of 89,134, 145, 163, and 180, was identified as caffeic acid [74,[78][79][80]82,83]. Furthermore, Peak 5 was tentatively identified as having three overlapping phenolic compounds.…”

Section: Peak Amentioning

confidence: 99%

Low-Fouling Plate-and-Frame Ultrafiltration for Juice Clarification: Part 1—Membrane Preparation and Characterization

et al. 2023

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Membrane technology provides advantages for separating and purifying food materials, including juice clarification. Ultrafiltration processes for fruit juices aim to remove haze components and maintain the stabilization of the juices. However, the membrane process during the clarification of fruit juices suffers from fouling, which deteriorates the process performance and products. Biofouling usually is found in the applications of the membrane in food processing. In this study, phenolic substances extracted from garlic bulbs are incorporated into a matrix of polymeric membranes to prevent fouling during juice clarification. Hydrophilic cellulose acetate was used as the polymer matrix of the membrane, and dimethylformamide was used as the solvent. The phenolic substances from garlic bulbs were incorporated into polymer solutions with different concentrations of 0%, 1%, 1.25%, and 1.5% w/v. The composite membrane was prepared using the phase inversion method, and the resulting membranes were later characterized. The results show that incorporating those phenolic compounds as the dope solution additive resulted in membranes with higher hydrophilic properties. They also modeled antibacterial properties, as shown by the reduced attachment of Bacillus subtilis of up to 91.5 ± 11.7% and Escherichia coli of up to 94.0 ± 11.9%.

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“…Beside these, the presence of some other polyphenols such as (syringic acid, osmanthuside A, garcimangosone C, 8-acetoxy-4′-methoxypinoresinol, protoaphin aglucone, (E)-1-[4-hydroxy-3-(3-methyl-1,3-butadienyl)phenyl]-2-(3,5-dihydroxyphenyl)ethylene, grossamide, urolithin A 3,8-O-diglucuronide, and dihydromyricetin) are also observed in DgM and DgB extracts Table 5. Fedosov et al (2016) showed eight phenolic compounds in fresh garlic methanolic extract, that is, caffeic acid, chlorogenic acid, rutin, hyperoside, apigenin 7-glucoside, luteolin 7-glucoside, rosmarinic acid, and luteolin, by utilizing HPLC GC/MS. Interestingly, in our study, various forms of the fresh HG17 extracts (FgM, FgB, FgEA; Table 5 Gladiatoside B2…”

Section: Phytochemical Analysis Of Hg17 Through Hr-lcms-qtofmentioning

confidence: 99%

Comparative study of antioxidant activities of Allium sativum (a novel variety, HG17) and Allium ampeloprasum (SMG): Revealing the higher potential of HG17 and analyzing its phytochemicals

Monika,

Dua,

Sharma

et al. 2024

Journal of Food Science

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Garlic, belonging to the genus Allium, is renowned for its rich antioxidant potential. Snow Mountain garlic (SMG) (Allium ampeloprasum) has been traditionally used for medicinal purposes because of its higher antioxidant potential. Considering its potential in medical therapies, we compared the antioxidant activity of SMG with a novel variety of Allium sativum, Hisar garlic 17 (HG17). Comparative antioxidant activity data (2,2‐diphenyl‐1‐picrylhydrazyl and 2,2‐azinobis (3‐ethylbenzothiazoline‐6‐sulfonic acid) revealed the higher antioxidant activity of HG17 than SMG, which prompted us to conduct a comprehensive phytochemical investigation to elucidate the factors contributing to antioxidant potential of HG17. To get a detailed antioxidant and phytoconstituents profiling, we differentially extracted HG17 by processing it in different forms (fresh, dry, heated, and aged) with two solvents (50% methanol and n‐butanol). Our data (antioxidant activities, total phenolics, and flavonoids) showed that dry garlic methanolic extract (DgM) had maximum potential than other HG17 forms/solvents, which concludes that different extraction techniques had direct impact on the phenolics/flavonoids and antioxidant potential of the extracts. Further, phytochemical analysis of HG17 extracts by high resolution liquid chromatograph mass spectrometer quadrupole time of flight validated the maximum potential of DgM. LCMS revealed the presence of garcimangosone C, osmanthuside A, and protoaphin aglucone polyphenols exclusively in DgM compared to other HG17 extracts, which possibly contributing in its high antioxidant potential. The overall differential extraction and LCMS data of HG17 strongly depict that it may be used as an alternative of SMG under diverse medical applications. HG17 higher antioxidant potential and rich array of unique phytochemicals make it valuable for food and pharmaceutical industries to integrate into functional foods/therapeutics.Practical ApplicationGarlic unique phytochemical composition and its remarkable ability to scavenge different radicals make it valuable therapeutic asset to mitigate diseases associated with oxidative stress. SMG is well known for its anti‐arthritic and anti‐inflammatory properties. HG17 showed higher antioxidant potential than SMG and can be used as an alternative of SMG for anti‐arthritic properties.

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The determination of phenolic compounds in artichoke flower head extracts by HPLC GC/MS technique

Cited by 5 publications

References 8 publications

In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials

In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials

Low-Fouling Plate-and-Frame Ultrafiltration for Juice Clarification: Part 1—Membrane Preparation and Characterization

Comparative study of antioxidant activities of Allium sativum (a novel variety, HG17) and Allium ampeloprasum (SMG): Revealing the higher potential of HG17 and analyzing its phytochemicals

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