The Determination of Moleculer Diversity Among Some Alfalfa (Medicago sativa L.) Ecotypes Using RAPD Markers

Abstract

Abstract:The relationships among total 76 ecotypes of alfalfa (Medicago sativa L.), 70 landraces and 6 cultivars, collected in Van and neighboring provinces were investigated. The experiment was established as an augmented design in 2009. In the molecular method, seventeen RAPD primers were used and 106 polymorphic bands were obtained. The genetic distances between the ecotypes were expressed by Euclidean coefficients. The genetic variation among alfalfa ecotypes was examined in 16 groups based on the localities, landraces and check cultivars. The highest genetic variations and polymorphisms were found in Ercis and Gurpinar localities H = 0.179, I = 0.277, H = 0.173, I = 0.267 and 62.26%, 59.43%, respectively. As a result, high genetic diversity was found out among the ecotypes and cultivars of allogamous Medicago sativa L.

Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers

“…The measurement of the OD 280 nm was used to define the content and the ratio OD260/OD280 was between 1.8 and 2.0 (Touil et al, 2008). The 20 μl master mix for the RAPD and ISSR analysis Five RAPD and four ISSR primers were selected from the previous studies on Medicago sativa L. (Denghan-Shoar et al, 1997;Gherardi et al, 1998;Mengoni et al, 2000;Paredes et al, 2002;Touil et al, 2008;Tucak et al, 2008;Petolescu and Nedelea, 2009;Ertus et al, 2014). RAPD amplification was performed in thermo-cycler starting with 4 min.…”

Genetic Characterization of Some Species of Vetch (Vicia L.) Grown in Turkey With SSR Markers

“…Determination of the diversity among genotypes is of great importance for breeding studies (Nunome et al 2009). In recent years, molecular markers have increased in genetic diversity and characterization studies (O'Neill et al 2003;Nunome et al 2009;Ozsensoy & Kurar 2012;Ertus et al 2016;Zulkadir & İdikut 2021). Among the molecular markers, SSR markers are the most widely used markers (Tautz 1989;Mengoni et al 2000) because of the high abundance in the genome, high polymorphism information content, random distribution within the genome, and its co-dominant inheritance (Dutta et al 2011).…”