The Determination of Collagen and Elastin in Tissues, With Results Obtained in Various Normal Tissues From Different Species

Lowry, Oliver H.; Gilligan, D. Rourke; Katersky, Evelyn M.

doi:10.1016/s0021-9258(18)72951-7

Cited by 235 publications

(21 citation statements)

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“…Skin is among the densest tissues within the body and therefore most likely to elicit cytothripsis, given that both epidermis and dermis have many highly confined spaces. Although collagen content is a main determinant of tissue density in the dermis (Lowry et al, 1941), other environmental constraints, such as the cellular density and tight junctions in the epidermis, likely also define the migratory capacity of leukocytes in tissues. Tissues of intermediate density, such as the walls of large arteries, may also elicit cytothripsis, thereby contributing to the vasculopathy associated with local virus reactivation that has been documented in some DOCK8deficient patients (Sabry et al, 2014;unpublished data).…”

Section: Dock8 Regulates Skin Tissue-resident Memory Cell Formation For Protection Against Viral Infectionmentioning

confidence: 99%

“…In contrast, the dermis is composed of a dense network of packed collagen fibers, through which immune cells must navigate (Wolf et al, 2009;Honda et al, 2014). The collagen fibers make up as much as one third of the wet weight of skin, as compared with 10% of aorta or 1% or less of other organs such as spleen and brain (Lowry et al, 1941;Neuman and Logan, 1950). Thus, the extracellular environments of the epidermis and dermis are characterized by many highly confined spaces, which are likely to tax the structural integrity of cells navigating to their targets.…”

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confidence: 99%

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DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity

Zhang

Dove

Hor

et al. 2014

Journal of Experimental Medicine

148

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Section: Dock8 Regulates Skin Tissue-resident Memory Cell Formation For Protection Against Viral Infectionmentioning

confidence: 99%

mentioning

confidence: 99%

DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity

Zhang

Dove

Hor

et al. 2014

Journal of Experimental Medicine

148

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“…The insoluble residue was suspended in 10 volumes of distilled water and autoclaved at 30 psi for 6 hr (19). This treatment extracts collagen as gelatin.…”

Section: Determination Of the Alkali-in-s O L U B L E E L A S T I Nmentioning

confidence: 99%

The Changes in Chemical Composition During Development of the Bovine Nuchal Ligament

Cleary

Sandberg

Jackson

1967

The Journal of Cell Biology

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Whole bovine nuchal ligaments, or portions thereof (in the case of commercially valuable animals), were obtained from 45 animals (28 fetal and 17 postnatal) ranging in age from 1 l0 days of gestation to l0 yr. Insoluble elastin was quantitativcly prepared from the fresh ligaments by extraction with hot alkali and by a combination of multiple extractions with alkaline buffer and then repeated autoclaving. When adult samples were examined, the yields of insoluble residue by these two methods were very similar, but with young fetal samples the second method gave significantly higher values, because of incomplete purification of the elastin residue. The changes in the concentration of collagen, alkali-insoluble elastin, and DNA have been examined. DNA concentration, and, thus, ccll population density, fell progressively during thc fetal period of development, to reach a steady value soon after birth. Collagen appeared in appreciable quantities before clastin, but its concentration was rapidly halved at about the time of birth. Insoluble elastin concentration was low until the end of the 7th fetal month, at which time it began to rise rapidly. The rate of increase in elastin concentration remained high throughout thc next 10-12 wk, by which time the adult value had been reached. Quantitative studies, on the basis of the whole ligament, showed that the total cell content rises to a maximum at birth, but falls soon after to a level about half that at birth. Total collagen production and elastin deposition continue at a steady, maximal rate over the interval from 235 days of gestation to the end of the 1st postnatal month. It is concluded that the immediate postnatal period would be the most favorable phase in which to attempt the isolation of the soluble precursor elastin.

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“…The residual fraction was prepared from the material centrifuged down at low speed after the extraction of actomyosin. The residue was extracted six to ten times by being ground in a mortar with 0-1 N-NaOH at 40 to extract all proteins except collagen and elastin (Lowry, Gilligan & Katersky, 1941). One of the extractions was overnight and the others were each for 30 min.…”

mentioning

confidence: 99%