The determination of ascorbic acid and uric acid in human seminal plasma using an HPLC with UV detection

A Randomized Steady-State Bioavailability Study of Synthetic versus Natural (Kiwifruit-Derived) Vitamin C

“…Although the baseline seminal fluid ascorbate was lower than vitamin C replete and healthy non-smoking men [35,36,37], little change was observed with the low dose vitamin C tablet or half kiwifruit per day dose. Early studies have shown that vitamin C intakes of up to 250 mg/day are required to return depleted seminal ascorbate to normal levels [35,36,38], indicating that higher intakes of vitamin C, e.g., those that result in plasma saturation, are required to increase seminal fluid levels [39].…”

Folin–Ciocalteu spectrophotometric assay of ascorbic acid in pharmaceutical tablets and orange juice with pH adjustment and pre‐extraction of lanthanum(III)–flavonoid complexes

“…Many analytical procedures have been developed and improved for the accurate and specific determination of ascorbic acid in pharmaceuticals and other natural samples that are important in the human diet. Methods of analysis include spectrophotometry, titrimetry, voltammetry, potentiometry, pulse polarography, fluorimetry, liquid chromatography, and flow injection . In spite of the developments in instrumental analytical resources, colorimetric–spectrophotometric methods of AA determination are still of interest due to their low cost, simplicity, and adaptability to optical sensors.…”

Quantitative determination of uric acid using CdTe nanoparticles as fluorescence probes

“…Thus, the detection of uric acid is of great importance in many areas including physiological investigations and disease diagnosis. The need to detect uric acid has led to the development and extensive use of an assortment of analytical methods, such as fluorescence (Zhang et al 2006;Zhang et al 2011), electrochemical analysis (Chen et al 2005;El Bouhouti et al 2009;Nassef et al 2007;Zare et al 2006), HPLC (Dai et al 2007;George et al 2006), chemiluminescence (Yang and Zhang 2010;Yu et al 2011), capillary electrophoresis (CE) (Causse et al 2007;Zhao et al 2008;Zinellu et al 2004), UV/colorimetric analysis (Schrenkhammer and Wolfbeis 2008), enzymatic-UV absorption (Zhao et al 2012), cyclic voltammetry (Noroozifar et al 2013), potentiometric non-enzymatic methods (Guan et al 2014), and methods based on HPLC-UV (Kanďár et al 2011). However, most of the existing methods are noted for their laborintensive and time-consuming procedures and costly instrumentation requirements; for instance, electro-analytical methods for determining uric acid are complicated by problems associated with selectivity and sensitivity.…”