The determination of antimicrobial and antibiofilm activities of foodborne lactic acid bacteria against Enterobacter cloacae isolates

Kenar, Beytullah; ERİK, Mine; Erdoğmuş, Sevim Feyza; Korcan, Safiye Elif; Köse, Zahide; Durmaz, Gül

doi:10.3906/vet-1905-100

Cited by 5 publications

(2 citation statements)

References 48 publications

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“…The amount of crystal violet measured OD was measured at 570 nm. The antibiofilm activity of the extracts was calculated with the percent reduction formula (Kenar et al, 2020).

Inhibition (() %) = \frac{normalA control - normalA sample}{normalA control} normalx 100

A control: Absorbance value not containing AgNPs.…”

Section: Methodsmentioning

confidence: 99%

Cyto–genotoxicity, antibacterial, and antibiofilm properties of green synthesized silver nanoparticles using Penicillium toxicarium

Korcan

Kahraman

Açıkbaş

et al. 2021

Microscopy Res & Technique

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The fungi are becoming the distinguished organisms utilized in the biological synthesis of metallic nanoparticles because of their metal bioaccumulation ability. Addressed herein, the extracellular synthesis of silver nanoparticles (AgNPs) was carried out by using the cell‐free filtrate of Penicillium toxicarium KJ173540.1. P. toxicarium was locally isolated and identified using both classical and molecular methods according to ribosomal internal transcribed spacer area of 18S rDNA. The optimum conditions for the AgNPs synthesis were found as 0.25 mM AgNO3 concentrations with pH 12 values at 45°C after 64 hr incubation in dark. Biosynthesized AgNPs were characterized via microscopic and spectroscopic techniques such as transmission electron microscopy, scanning electron microscopy, energy dispersive X‐ray analysis, Fourier transform infrared spectrophotometer, and ultraviolet–visible spectroscopy. Zetasizer measurements presented that the high negative potential value (−18.1 mV) and PDI (0.495) supported the excellent colloidal nature of AgNPs with long‐range stability and high dispersity. AgNPs exhibited cyto–genotoxicity in Allium cepa root meristem cells by decreasing mitotic index and increasing chromosome aberrations in a dose‐dependent manner. Then, 100 and 50% concentration of biosynthesized AgNPs showed antibacterial activity on Staphylococcus aureus and Bacillus subtilis. A decreasing biofilm formation of Pseudomonas aeruginosa 80.69, 48.32, and 28.41% was also observed at 100, 50, and 25% of mycosynthesized AgNP, respectively.

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“…The amount of crystal violet measured OD was measured at 570 nm. The antibiofilm activity of the extracts was calculated with the percent reduction formula (Kenar et al, 2020).

Inhibition (() %) = \frac{normalA control - normalA sample}{normalA control} normalx 100

A control: Absorbance value not containing AgNPs.…”

Section: Methodsmentioning

confidence: 99%

Cyto–genotoxicity, antibacterial, and antibiofilm properties of green synthesized silver nanoparticles using Penicillium toxicarium

Korcan

Kahraman

Açıkbaş

et al. 2021

Microscopy Res & Technique

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“…100 μl of aliquots from each well were transferred to a new microtiter plate and the optical density (OD) of each well was measured at 570 nm using a microplate spectrophotometer (Thermo Scientific Multiskan Sky). The medium was used as a negative control and P. aeruginosa ATCC 11778 that is known to generate biofilm was used as the positive control (Kenar et al, 2020). According to the critical OD value (ODc) the biofilm production was scored as: non-biofilm producers [(-), OD ≤ ODc], weak biofilm producers [(+), ODc < OD ≤ 2 x ODc], moderate biofilm producers [(++), 2 x ODc < OD ≤ 4 x ODc], or strong biofilm producers [(+++), OD > 4 x ODc] ( Gomes et al, 2019).…”

Section: Determination Of the Biofilm Formation Of Pathogen Test Micr...mentioning

confidence: 99%

The antibiofilm effects of some Cistus spp. against pathogenic microorganisms

Erdoğmuş

Bilecen

Altıntaş³

et al. 2022

ijpbp

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Recently, the potential antibacterial or antibiofilm effects of some plant species belonging to the Cistus sp. have motivated investigation of their use as herbal remedies. In this study, antibiofilm activities of aqueous (dH2O) leaf extracts of Cistus laurifolius L., C. creticus L. and C. salviifolius L. on some pathogenic microorganisms with biofilm forming ability were investigated. Biofilm forming ability of pathogen test microorganisms were evaluated by congo red agar method and microtiter plate method and all tested microorganisms were confirmed as biofilm producers. Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 11778, S. aureus ATCC 6538, P. aeruginosa ATCC 27853 and S. aureus ATCC 12600 were evaluated as strong biofilm producers. The highest concentration of examined extracts both showed biofilm inhibition and biofilm eradication against the tested pathogen microorganisms. In particular, studied plant extracts showed good antibiofilm effect, and biofilm eradication against S. aureus ATCC 6538 and S. aureus ATCC 12600. MBIC50 values of S. aureus ATCC 6538 were found as 6.25 µg/ml of C. laurifolius, and 50 µg/ml of C. creticus extracts. Also, 50 µg/ml of C. creticus extract showed ≥ 90% inhibition of biofilm growth (MBIC90 = 50 µg/ml). MBEC50 values of S. aureus ATCC 12600 were determined as 6.25 µg/ml in all tested plant extracts and 50 µg/ml of C. creticus extract was required to induce ≥ 90% eradication (MBEC90) of biofilm growth of S. aureus ATCC 12600. Our study revealed that aqueous leaf extracts of C. laurifolius, C. creticus and C. salviifolius could be potential candidates for drug discovery to treat pathogen test microorganisms capable to induce infectious diseases especially by their biofilm forming ability.

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