The determination of 5 anticoccidial drugs (nicarbazin, lasalocid, monensin, salinomycin and narasin) in animal livers and eggs by liquid chromatography linked with tandem mass spectrometry (LC-MS-MS)

Abstract

A method has been developed for the simultaneous extraction and determination of five of the most commonly used anticoccidial drugs (nicarbazin, lasalocid, monensin, narasin and salinomycin) from livers and eggs by LC-MS-MS. Results show good repeatability, with mean spiked recoveries for nicarbazin, lasalocid, monensin, narasin and salinomycin in poultry livers in the average range of 92-118%, and 86-110% in eggs. The detection limit is at 1 ng ml(-1) for all the named compounds and a quantitation level of 2.5 ng g(-1) has been achieved. A high throughput of samples is achievable using this method which allows the analysis of up to 40 samples by one analyst in a day.

A sensitive and validated HPLC–MS/MS method for simultaneous determination of seven coccidiostats in bovine whole milk

“…In the literature it can be easily found methods for the analysis of coccidiostats food matrices, such as liver (Capurro, Danaher, Anastasio, Cortesi, & O'Keeffe, 2005;Olejnic, Szprengier-Juszkiewicz and Jedziniak, 2009), muscle (Dubois, Pierret, & Delahaut, 2004;Matabudul, Conway, & Lumley, 2000) and egg (Dubois et al, 2004;Galarini, Fioroni, Moretti, Pettinacci, & Dusi, 2011;Matabudul, Lumley, & Points, 2002;Matabudul et al, 2000). However, and up to day, only a few methods permit the complete confirmation of coccidiostats in milk.…”

Cross-contamination of non-target feedingstuffs by nicarbazin authorised for use as a feed additive - Scientific opinion of the Panel on Contaminants in the Food Chain

“…A multi-residue LC-MS/MS method was developed to quantify nicarbazin (DNC) residue in sheep and chicken liver and eggs (Matabudul et al, 2002). After mixing sample with anhydrous sodium sulphate, residues were extracted with acetonitrile.…”

Liquid chromatography–tandem mass spectrometric assays for salinomycin in mouse plasma, liver, brain and small intestinal contents and in OptiMEM cell culture medium

“…Sensitive analytical methods (lower limit of quantification (LLQ) ≤ 10 ng/g) that have been reported for salinomycin to date, generally use liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) and have been employed for eggs [15][16][17][18][19][20][21], tissues (liver, muscle and fat) [15][16][17]19,21,22], manure [23], surface water [24,25], soil [26] and sediment [25]. In addition, a time resolved fluorescence immunoassay for egg and muscle [27] and an LC-MS method for chicken egg, tissues and plasma [28] has been described.…”

“…In addition, a time resolved fluorescence immunoassay for egg and muscle [27] and an LC-MS method for chicken egg, tissues and plasma [28] has been described. All these assays were combined with the determination of other ionophores [15][16][17][18][19][20][21][22][23][24][25][26][27][28].…”