The Detection of the Accumulation of Silicon in Phalaenopsis (Orchidaceae)

Zhuo, Tian-Su

doi:10.1006/anbo.1995.1065

Cited by 19 publications

(15 citation statements)

References 4 publications

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“…Zhou (1995) confirms that the presence of silicate provides to the plants greater resistance to water loss when compared to the control treatment, providing better arrangement of epidermal cells without loss by dehydration of the tissues, which would harm the acclimatization process.…”

Section: Resultssupporting

confidence: 56%

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

et al. 2017

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The aim of this study was to compare vitroplants Catasetum x apolloi grown under natural light and artificial light and different concentrations of potassium silicate, providing data on the anatomical differentiation that aids the acclimatization process of this species. Plants from in vitro seeding were used; 5 protocorms of approximately 0.5 cm were inoculated into vials with a capacity of 500 mL containing 100 mL of alternative culture medium plus potassium silicate (0.0, 0.5; 1.0 mL L -1 ), pH adjusted to 5.5 ± 0.5 and gelated with 4GL -1 agar before the autoclaving process. Cultures were maintained under natural light (TNE) and artificial light (TAE) for 90 days, and micromorphometric analysis was performed for polar and equatorial diameter, density and stomatal index, blade thickness in the central rib, and secondary veins. Applications in K 2 SiO 4 alternative medium provided the following: elongation of the hypodermis, thicker mesophyll, and more prominent midrib; elipptical guard cells; formation of epistomatal chamber; and lower stomatal density and stomatal with lower equatorial and polar diameters. The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses.Keywords: leaf anatomy, light, orchids, plants vitro. Anatomia e análise micromorfométrica foliar de Catasetum x apolloi Benelli & Grade com adição de silicato de potássio em diferentes fontes de luzResumo O objetivo desse trabalho foi comparar vitroplantas de Catasetum x apolloi cultivadas sob luz natural e luz artificial e diferentes concentrações de silicato de potássio, fornecendo dados sobre diferenciação anatômica que auxiliem no processo de aclimatação dessa espécie. Utilizou-se plantas provenientes da semeadura in vitro, 5 protocormos de aproximadamente 0,5 cm foram inoculados em frascos com capacidade para 500 mL contendo 100 mL de meio de cultura alternativo, acrescido de silicato de potássio (0,0; 0,5; 1,0 mL L -1 ), pH ajustado para 5,5 ±0,5 e gelificado com 4gL -1 de ágar antes do processo de autoclavagem. As culturas foram mantidas sob luz natural (TAA) e luz artificial (TAN) por 90 dias, e feitas análises micromorfométricas (diâmetro polar e equatorial, densidade e índice estomático, espessura do limbo na nervura central e nervuras secundárias). As aplicações de K 2 SiO 4 em meio alternativo, propiciaram: alongamento da hipoderme; mesofilo mais espesso e nervura central mais proeminente; células guardas elípticas; formação de câmaras supraestomáticas; menor densidade estomática e estômatos com menores diâmetros equatorial e polar. As condições que podem favorecer a aclimatação são menores intensidades de luz e menores doses de silicato de potássio.Palavras-chave: anatomia foliar, luminosidade, orquídeas, vitroplantas.

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Section: Resultssupporting

confidence: 56%

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

et al. 2017

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“…Em tomateiro, Lana et al (2003), ao utilizar doses crescentes de CaSiO 3 no solo, não verifi caram efeito positivo na produtividade. Zhou (1995) Pela análise anatômica de uma folha, é possível demonstrar a presença de depósitos de silício, comumente chamados de silicofi tólitos, que ocorrem na parede celular como incrustação e/ou impregnação, ou ainda, sob a forma de corpos silicosos (opala) no interior das células de diferentes tecidos (Metcalfe, 1983).…”

Section: Resultsunclassified

Características anatômicas de mudas de morangueiro micropropagadas com diferentes fontes de silício

Braga

Nunes

Favero

et al. 2009

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o efeito de diferentes fontes de silício, utilizadas na micropropagação, nas características anatômicas de mudas de morangueiro (Fragaria x ananassa). Foram utilizados propágulos da cv. Oso Grande cultivados em meio Murashige e Skoog (MS), acrescido de 30 g L -1de sacarose, 6 g L -1 de ágar e 1 mg L -1 de 6-benzilaminopurina. Os tratamentos consistiram da adição ao meio MS dos silicatos de cálcio, de sódio e de potássio, na dosagem de 1 g L -1 . O tratamento testemunha foi o meio MS sem fonte de silício. O delineamento experimental foi o inteiramente ao acaso, com dez repetições. Os propágulos foram mantidos por 45 dias em sala de crescimento, em condições controladas. Avaliaram-se características fi totécnicas e anatômicas dos propágulos in vitro. Verifi cou-se que o aumento da massa de matéria fresca e seca dos propágulos de morangueiro ocorreu na presença de silicato de sódio. A suplementação do meio de cultura com silício proporcionou maior teor de clorofi la. A adição de silicato de sódio ao meio MS resultou em aumento da espessura dos tecidos do limbo foliar e da deposição de cera epicuticular e na formação de depósito de silício nas células.Termos para indexação: Fragaria x ananassa, cera epicuticular, micropropagação in vitro, silicato. Anatomical characteristics of the strawberry seedlings micropropagated using different sources of siliconAbstract -The objective of this work was to evaluate the effect of different silicon sources, used in micropropagation, on the anatomical characteristics of strawberry's (Fragaria x ananassa) seedlings. Propagules of cv. Oso Grande were cultivated on a Murashige and Skoog (MS) medium containing 30 g L -1 of sucrose, 6 g L -1 of agar and 1 mg L -1 of 6-benzylamino purine. The treatments consisted of calcium, sodium, or potassium silicates added to the MS medium, at the dosage of 1 g L -1. The MS medium without added silicon was the check treatment. The experimental design was completely randomized with ten replications. The propagules were maintained during 45 days in a growth chamber, under controlled conditions. Developmental and anatomical characteristics of the in vitro propagules were evaluated. Fresh and dry weight of the propagules increased in the presence of sodium silicate. The supplementation of the culture medium with silicon increased the content of chlorophyll. The addition of sodium silicate in the MS medium resulted in increased thickness of leaf tissues and epicuticular wax deposition and in increased silicon deposit in the cells.Index terms: Fragaria x ananassa, epicuticular wax, in vitro micropropagation, silicate.

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“…Zhou (1995) observed silica bodies in leaf tissues of Phalaenopsis hybrid plantlets grown in Vacin and Went medium supplemented with CaSiO 3 . Addition of CaSiO 3 also increased the leaf length.…”

Section: Growth and Developmentmentioning

confidence: 87%

The role of silicon in plant tissue culture

Sivanesan¹,

Park²

2014

Front. Plant Sci.

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Growth and morphogenesis of in vitro cultures of plant cells, tissues, and organs are greatly influenced by the composition of the culture medium. Mineral nutrients are necessary for the growth and development of plants. Several morpho-physiological disorders such as hooked leaves, hyperhydricity, fasciation, and shoot tip necrosis are often associated with the concentration of inorganic nutrient in the tissue culture medium. Silicon (Si) is the most abundant mineral element in the soil. The application of Si has been demonstrated to be beneficial for growth, development and yield of various plants and to alleviate various stresses including nutrient imbalance. Addition of Si to the tissue culture medium improves organogenesis, embryogenesis, growth traits, morphological, anatomical, and physiological characteristics of leaves, enhances tolerance to low temperature and salinity, protects cells and against metal toxicity, prevents oxidative phenolic browning and reduces the incidence of hyperhydricity in various plants. Therefore, Si possesses considerable potential for application in a wide range of plant tissue culture studies such as cryopreservation, organogenesis, micropropagation, somatic embryogenesis and secondary metabolites production.

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The Detection of the Accumulation of Silicon in Phalaenopsis (Orchidaceae)

Cited by 19 publications

References 4 publications

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

Características anatômicas de mudas de morangueiro micropropagadas com diferentes fontes de silício

The role of silicon in plant tissue culture

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