The detection of Dichelobacter nodosus and Fusobacterium necrophorum from footrot lesions in New Zealand goats

Bennett, Grant; Loenen, Ayla L. van; Zhou, Huitong; Sedcole, Richard; Hickford, Jonathan G. H.

doi:10.1016/j.anaerobe.2009.02.003

Cited by 7 publications

(6 citation statements)

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“…These figures suggest that PCR has 1% false-negative and 15% true-positive rates compared to culture, indicating superiority of the PCR method to the culture in agent identification. Our findings for D. nodosus (Moore et al 2005;Bennett et al 2009a;Rather et al 2011) and F. necrophorum (Bennett et al 2009a(Bennett et al , 2009cZhou et al 2009) were inconsistent with literature. Inconsistency may be caused by the severity of disease, involvement of other bacteria, sampling collection protocol, and the production system.…”

Section: Discussioncontrasting

confidence: 57%

Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

et al. 2015

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Footrot is an important infectious disease of small ruminants leading to severe economical losses. The aim of the present study was to determine isolation and identification rates of Dichelobacter nodosus and Fusobacterium necrophorum in the culture techniques and reveal the specificity and sensitivity of the culture technique based on the polymerase chain reaction (PCR) method in sheep with footrot. Dry swabs and swabs with Amies medium from 83 sheep were subjected to PCR and culture analyses. In dry swabs, 4 samples were positive for F. necrophorum and all were negative for D. nodosus. Colonies in Eugon and Fusobacterium selective agars from swabs with Amies medium were evaluated. Polymerase chain reaction analysis was conducted on macroscopically and microscopically unidentified samples. The positivity rate was 55.4% for D. nodosus and 69.8% for F. necrophorum in cultures from Fusobacterium selective agars. The positivity rate for D. nodosus in Fusobacterium selective agars was higher than that in Eugon agar. Performing PCR and culture methods increased positivity as compared to performing them alone. In comparison with the PCR method, culturing in Fusobacterium selective agars had moderate sensitivity and low specificity for D. nodosus (71.7 and 28.7%) and F. necrophorum (61.3 and 80.0%), respectively. In conclusion, Fusobacterium selective agar (without antibiotics) for isolation and identification of D. nodosus is superior to Eugon agar. Fusobacterium necrophorum should also be considered as a provoking agent for footrot in small ruminants. The PCR method on culture increases elucidation of definitive aetiology.

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Section: Discussioncontrasting

confidence: 57%

Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

et al. 2015

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“…The grams staining of bacterial smears have been showed the characteristic bacterial morphology of Fusobacterium necrophorum and they were observed as gram-negative, long, non-branched filamentous pleomorphic bacilli rang about 100µm in diameter with parallel sides and blunt or tapering ends . 4 The result of PCR technique for detection of Fusobacterium necrophorum .sub necrophorum, leukotoxin gene (lktA), in (80) foot rot swab samples of the affected sheep) for the extracted DNA from all the isolated bacteria that electrophorised with 1.5 % agarose gel have been showed positive samples with Fusobacterium necrophorum .sub necrophorum, leukotoxin gene (lktA), in analysis appeared as single band under the U.V light with the length 403 bp. (Figure 2) of amplified DNA of all strains of Fusobacterium necrophorum .sub necrophorum.…”

Section: Resultsmentioning

confidence: 99%

A Concordance Study between Polymerase Chain Reaction Assay and Conventional Culture-based Methods for Detection of Fusobacterium necrophorum

Saleh¹,

Muhammed²,

Alnasrawi³

2020

IJFMT

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Foot rot duo to Fusobacterium necrophorum is an important clinical economic disease in ovine, in order to inform identification and antibiotics selection, Conventional culture-based method and Polymerase chain reaction assays had been proposed to provide a good inter-rater reliability between these techniques, this study was aimed to differentiate the accuracy of conventional culture-based method and PCR technique for identification of Fusobacterium necrophorum in an ovine foot rot. Method:The current study was conducted on three farms of Kerbala governorate (AL-husseinyia, Al-Hurr and Ain Al-tmor cities) during the period from October 2017 to April 2018, pathological swab samples of the interdigital region of affected (n= 40) sheep and (n=40) goat were enrolled in this study, after the collection of swabs, the samples were divided into two partitions, each swab of the first partition was immediately collected into lighten tube containing 5 mL of thioglycollate broth enriched with 0.5% ground sheep and was transmitted to the laboratory for conventional culture-based method and the another partition of swabs was sealed with other cooled tubes containing 5 mL of thioglycollate broth enriched with 0.5% ground sheep for the laboratory of molecular investigations.Results: Fusobacterium necrophorum was identified in the pathological samples by the two following depended assays: and (46) swabs were positive by conventional culture-based method and (44) swabs were positive with PCR assay, Cohen's kappa coefficient revealed P value for conventional culture-based methods vs. PCR assay of 0.056 and the current study was demonstrated a highly identification rates of Fusobacterium necrophorum in conventional culture-based methods rather than PCR assay. Conclusions:Current study documented conventional culture-based method and PCR assays were facilitate the identification of Fusobacterium necrophorum in case of presumed ovine foot rot, it also concluded that molecular assays should be depended in confirmatory detection of F. necrophorum in affected animals.

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“…In the scientific literature, there are few reports on Dichelobacter nodosus (foot rot) and Treponema spp. infections (Mortellaro) in goats in the United Kingdom [20,21], Brazil [22] and New Zealand [23,24]. However, little is known about infectious claw diseases in goats in general and their prevalence in Switzerland.…”

Section: Introductionmentioning

confidence: 99%

Influence of Housing and Management on Claw Health in Swiss Dairy Goats

Sailer

Holinger

Burla

et al. 2021

Animals

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Due to a rising demand for goat milk and goat milk products worldwide, it is likely that dairy goat production will be intensified in the future, with larger herds per farm. In Switzerland, as in many other countries with intensive farming systems, dairy goats are typically housed on deep litter, with little access to hard abrasive surfaces. Such housing conditions will result in wall horn overgrowth. The aim of this study was to gain profound knowledge on the occurrence of overgrown wall horn, its impact on claw health and locomotor behavior, and possible adverse effects on animal welfare. Additionally, housing and management factors that may contribute to non-physiological claw conditions were evaluated. To compare claw conditions after the summer grazing period and the winter indoor housing period, data were collected on 28 Swiss dairy goat farms in autumn and spring (621 goats in total). Claw lesions were recorded with the help of a “claw card” documenting each claw. Furthermore, pictures were taken of each claw to determine the severity of wall horn overgrowth. Locomotion behavior (activity, lying time and lying bouts) was recorded with three-dimensional accelerometers fixed to the goats’ hind legs. In autumn, 66.7% of the examined claws showed moderate overgrowth, 32.4% severe overgrowth and 0.9% no overgrowth. In spring, 47.4% of the examined claws were affected with moderate overgrowth, 52.6% with severe overgrowth and 0.0% with no overgrowth. Horn separation (48.1% of examined claws) and sole hemorrhages (16.0% of examined claws) were the most frequent lesions. In goats with severely overgrown claws, the risk of developing sole hemorrhages was doubled compared with moderate overgrowth. The occurrence rate of horn separation was lower if the trimmer had attended a special skills training course (p < 0.001). Furthermore, locomotor activity (p < 0.01) and the number of lying bouts per day (p < 0.01) were higher in spring than autumn. Neither the goats’ activity nor the number of lying bouts per day differed before and after claw trimming. Finally, season and trimming were not associated with the goats’ total lying time. A certain extent of wall horn overgrowth in dairy goat claws cannot be avoided under the housing conditions typical for Swiss farms. Severe wall horn overgrowth is associated with an increase in the proportion of claws with sole hemorrhages. Therefore, regular and careful functional claw trimming, taking the housing situation (deep bedding, access to pasture, grazing on alpine pasture) into account, should be promoted.

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The detection of Dichelobacter nodosus and Fusobacterium necrophorum from footrot lesions in New Zealand goats

Cited by 7 publications

References 1 publication

Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

A Concordance Study between Polymerase Chain Reaction Assay and Conventional Culture-based Methods for Detection of Fusobacterium necrophorum

Influence of Housing and Management on Claw Health in Swiss Dairy Goats

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