Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

Abstract

Footrot is an important infectious disease of small ruminants leading to severe economical losses. The aim of the present study was to determine isolation and identification rates of Dichelobacter nodosus and Fusobacterium necrophorum in the culture techniques and reveal the specificity and sensitivity of the culture technique based on the polymerase chain reaction (PCR) method in sheep with footrot. Dry swabs and swabs with Amies medium from 83 sheep were subjected to PCR and culture analyses. In dry swabs, 4 samples were positive for F. necrophorum and all were negative for D. nodosus. Colonies in Eugon and Fusobacterium selective agars from swabs with Amies medium were evaluated. Polymerase chain reaction analysis was conducted on macroscopically and microscopically unidentified samples. The positivity rate was 55.4% for D. nodosus and 69.8% for F. necrophorum in cultures from Fusobacterium selective agars. The positivity rate for D. nodosus in Fusobacterium selective agars was higher than that in Eugon agar. Performing PCR and culture methods increased positivity as compared to performing them alone. In comparison with the PCR method, culturing in Fusobacterium selective agars had moderate sensitivity and low specificity for D. nodosus (71.7 and 28.7%) and F. necrophorum (61.3 and 80.0%), respectively. In conclusion, Fusobacterium selective agar (without antibiotics) for isolation and identification of D. nodosus is superior to Eugon agar. Fusobacterium necrophorum should also be considered as a provoking agent for footrot in small ruminants. The PCR method on culture increases elucidation of definitive aetiology.

Kars Yöresi Koyunlarında Piyeten Olgularından Dichelobacter nodosus İzolasyonu ve PCR İle İdentifikasyonu

“…In spite of this variety of geography, there are very few studies that isolate and identify the agent for ovine footrot due to the difficulty of culturing many of the microbes that cause foot diseases in cloven hoof animals (anaerobiosis, fragility, mixed infection etc.) [17] . Previous studies have been largely focused on the healing effects of different diagnosis and treatment options using clinical radiological imaging.…”

Van Muradiye İlçesinde Ruminantlarda Görülen Ekstremite ve Ayak Hastalıklarının İnsidansı

“…1989; Sağlıyan ve ark. 2008;Özgen ve ark. 2015).İzci (1994), yaptığı çalışmada ağıl dönemi sonunda piyeten oranını %0.14, mera dönemi sonunda ise %0.64 olarak saptamıştır.Yıldız ve Gençcelep (2021), Muş ve yöresinde yaptıkları çalışmada piyeten insidansını %9.14 olarak belirlemiştir.…”