The detection of blood contamination in human follicular fluid

Levay, PF; Huyser, Carin; Fourie, F. Le R.; Rossouw, D. J.

doi:10.1007/bf02766112

Cited by 33 publications

(22 citation statements)

References 21 publications

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“…Only FF free of blood was used to determine hormone concentrations. For FF selection we used the visual criterion proposed by Levay et al (9). The FF volume obtained after aspiration was measured and the FF was immediately poured onto Petri dishes previously heated to 37ºC on a hot plate.…”

Section: Methodsmentioning

confidence: 99%

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Costa

Mendes

Ferriani

et al. 2004

Braz J Med Biol Res

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The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing mature oocytes (median = 368 and 5.7 ng/ml, respectively; P < 0.05). Progesterone, androstenedione and estrone levels were not significantly different between mature and immature oocytes. The application of the receiver-operating characteristic curve statistical approach to determine the best cut-off point for the discrimination between mature and immature oocytes indicated levels of 505.8 ng/ml for E2 (81.0% sensitivity and 81.8% specificity) and of 10.4 ng/ ml for testosterone (90.9% sensitivity and 82.4% specificity). Follicular diameter was associated negatively with E2 and testosterone levels in FF. There was a significant increase in progesterone/testosterone, progesterone/E2 and E2/testosterone ratios in FF containing mature oocytes, suggesting a reduction in conversion of C21 to C19, but not in aromatase activity. The overall fertility rate was 61% but there was no correlation between the steroid levels or their ratios and the fertilization rates. E2 and testosterone levels in FF may be used as a predictive parameter of oocyte maturity, but not for the in vitro fertilization rate.

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Section: Methodsmentioning

confidence: 99%

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Costa

Mendes

Ferriani

et al. 2004

Braz J Med Biol Res

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“…The remaining follicles were aspirated according to usual clinical procedures. No samples had evidence of trace red blood cell contamination upon visual inspection before or after centrifugation [24]. Two aliquots from each follicle were shipped to the University of Buffalo in New York State on dry ice via overnight service for biochemical analysis.…”

Section: Clinical Protocol and Specimen Collectionmentioning

confidence: 99%

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Kim

Bloom

Browne

et al. 2016

J Assist Reprod Genet

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Purpose Follicular redox balance is likely to be important for embryo quality during in vitro fertilization (IVF), and the anti-oxidative high desity lipoprotein (HDL) particle is the sole lipoprotein measured in follicular fluid (FF). Therefore, we investigated FF HDL particle components as predictors of embryo quality during IVF. Methods Two research follicles collected from each participant were individually tracked, and 103 women having at least one developed embryo were included in the analysis. Concentrations of 15 non-cholesterol HDL particle components and 26 HDL-cholesterol (HDL-C) particle size subfractions were determined. Embryo quality was assessed for embryo cell number, embryo fragmentation, and embryo symmetry. Multivariable Poisson regression with a sandwich variance estimator was used to evaluate associations between HDL particle components and embryo quality, adjusted for covariates. Results Higher γ-tocopherol concentration was associated with less embryo fragmentation (relative risk [RR] = 4.43; 95 % confidence interval [CI] 1.78, 11.06), and higher apolipoprotein A-1 concentration was associated with full embryo symmetry (RR = 3.92; 95 % CI 1.56, 9.90). Higher concentrations of HDL-C subfractions in the large and medium particle size ranges were associated with poorer embryo quality. Conclusions FF HDL lipophilic micronutrients and protein components, as well as HDL-C particle size, may be important predictors of embryo quality during IVF.

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“…The aspirated FF supernatant was aliquoted (0.6 mL) and frozen at −80°C. No specimens showed visual evidence of blood contamination before or after centrifugation [21]. We shipped two aliquots from each follicle to the University of Buffalo, State University of New York on dry ice via overnight service for biochemical analyses.…”

Section: Clinical Protocol and Specimen Collectionmentioning

confidence: 99%

Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles

Kim

Bloom

Fujimoto

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. Methods We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles.Results For most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ 2 F :%σ 2 B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies. Conclusions These preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.

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The detection of blood contamination in human follicular fluid

Cited by 33 publications

References 21 publications

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles

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