The detection of Aspergillus fumigatus antibodies in sera by immunogold-silver staining

Reijula, Kari; Kurup, Viswanath P.; Fink, Jordan N.

doi:10.1016/0167-7012(92)90075-f

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Elevated levels of specific IgG and IgE antibodies io A. fumigatus have been reported in several Aspergillusindueed diseases [21,22]. By using immunogold and immunogold-silver assay with light microscopy we have demonstrated A. fumigatus specific antibodies in the human sera [21,22]. Gold particles bound to the cell walls of fixed slide cultures of A. fumigatus co\s\Ci be visualized directly under a light microscope or after enhancement with silver.…”

Section: Discussionmentioning

confidence: 90%

Monoclonal antibodies bind identically to both spores and hyphae of Aspergillus fumigatus

Reijula

Kurup

Kumar

et al. 1992

Clin Experimental Allergy

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Immunoelectron microscopy (IEM) was used to determine the binding of six monoclonal antibodies (MoAbs) produced against Aspergillus fumigatus antigens present on or within the conidia and hyphae of the fungus. Antigen-antibody complexes were demonstrated in EM using labelled colloidal gold particles (15 nm). Three out of 6 MoAbs (C9, F12 and H10) reacted only with the cytoplasmic components of A. fumigatus while the remaining three (B12, F6G5 and D6E6) showed reactivity to both cytoplasm and cell wall of the conidia and hyphae. The results indicate that IEM is of considerable value in determining and selecting monoclonal antibodies having specific reactivity with diverse antigenic components.

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