The Detection and Identification of Pediococcus and Micrococcus in Breweries, Using a Serological Method

Abstract

A commercially-available antiserum for the identification of group D strains of Streptococcus contains antibodies which react with Pediococcus and Micrococcus, and provides a convenient method to detect these genera in yeast, wort and beer. After suitable adsorption it can be used to distinguish between Pediococcus and Micrococcus and, within each genus, to differentiate between groups normally found in a brewery environment and those from other sources.

The Use of the A.P.I. Lactobacillus System for the Characterization of Pediococci

“…Some strains are able to grow in fermenting wort and beer and are, thus, potential spoilage organisms. A general procedure for the detection of these organisms using the fluorescent antibody staining technique has been described and the possibility of using highly specific sera to distinguish the strains liable to cause spoilage from others has been discussed (Dolezil & Kirsop 1976). In this latter connection it is important to define the taxonomic position of the strains which are able to grow in beer.…”

Bacterial surface antigen-specific monoclonal antibodies used to detect beer spoilage pediococci

“…Serological methods using polyclonal antibodies have been applied in the brewing industry to identify pediococci, however, none of these methods are in current use (Claussen et al 1975(Claussen et al , 1981Dolezil and Kirsop 1976). To date, there have been no serological studies of surface antigens that contribute to taxonomy of the genus Pediococcus.…”

The Genera of Lactic Acid Bacteria

“…DNA probe methods are available for detection and identification of glucan-forming wine-spoilage strains of P. damnosus (Lonvaud-Funel et at., 1993), for non-glucan-forming strains of P. damnosus and P. pentosaceus in fermenting grape must and wine (Lonvaud-Funel et al, 1991), and for strains of P. pentosaceus that colonize silage (Cocconcelli et at., 1991). Dolezil and Kirsop (1976) used a commercially available antiserum from Group D streptococci to detect Pediococcus spp. in brewers' yeast, wort and beer.…”