The Use of the A.P.I. Lactobacillus System for the Characterization of Pediococci

Abstract

A commercially produced system for the identification of Lactobacillus, by which the reactions of concentrated cell suspensions towards 50 substrates may be determined, is useful for the characterization of strains of Pediococcus. Suitable conditions for inoculum preparation vary for slow and fast growing strains; the influence of cell concentration on the results is described. The reactions are classified in terms of their value for species characterization and the results are shown to agree well with those in other studies where classical procedures have been used.

Simulation of the Rat Intestinal Ecosystem using a Two-stage Continuous Culture System

“…The recovery of lactobacilli on pre-reduced RCM-blood agar (anaerobic incubation) was always less than that observed on Rogosa SL Agar. However, the numbers observed in the faeces were similar to those reported in other studies (Drasar et al, 1969). The rat hindgut ecosystem The species composition and community structure was determined for the faeces of five Wistar and two Sprague Dawley rats sampled at least five times a week over 7 months.…”

“…The API system provides a rapid and reliable means of routine species identification using standardized sets of biochemical tests (Dolezil & Kirsop, 1977 …”

“…Gastrointestinal ecosystems are complex with a high degree of interaction among the different components, resulting in a sustained capacity for self-regulation and homeostasis (Dubos et al, 1965;Savage, 1977;Fretner, 1974).…”

Bacterial surface antigen-specific monoclonal antibodies used to detect beer spoilage pediococci

“…In most laboratories, identification of Pediococcus isolates is still a time-consuming process done by comparing the phenotypic features of an unknown isolate to identification tables and keys (Priest 1987). Unfortunately, even with commercial tests like the API 50E CHL fermentation kit (Dolezil and Kirsop 1977), accurate identification is often impossible owing to the phenotypic similarity between many species, strain variability, and less than adequate data bases. Despite the difficulty in making a taxonomic identification of a Pediococcus isolate, what is known with certainty is that P. damnosus is the Pediococcus species most frequently found as a beer spoiler (Lawrence and Priest 1981).…”

The Genera of Lactic Acid Bacteria

“…DNA/DNA hybridization assays can also be used to confirm species identity (Back and Stackebrandt, 1978). Dolezil and Kirsop (1977) used the API lactobacillus system (now the API 50CH system) to discriminate between strains of pediococci. Lawrence and Priest (1981) confirmed the value of this kit for identification of brewery pediococci while Davis et at.…”