The Desirability Optimization Methodology; a Tool to Predict Two Antagonist Responses in Biotechnological Systems: Case of Biomass Growth and Hyoscyamine Content in Elicited Datura starmonium Hairy Roots

Amdoun, Ryad; Khelifi, Lakhdar; Khelifi-Slaoui, Majda; Amroune, Samia; Asch, Mark; Assaf-Ducrocq, Corinne; Gontier, Éric

doi:10.21859/ijb.1339

Cited by 90 publications

(53 citation statements)

References 39 publications

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“…A triplicate experiment was set up to validate the optimized condition. The composite desirability (0.993) is close to 1, which indicates the settings seem to achieve favourable results for all responses as a whole 50 . Based on predictive model, the optimum particle size is 44 µm to obtain favourable results of phytochemical composition and antioxidant activity.…”

Section: Resultsmentioning

confidence: 78%

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Prasedya

Frediansyah

Martyasari

et al. 2021

Sci Rep

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Sample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

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Section: Resultsmentioning

confidence: 78%

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Prasedya

Frediansyah

Martyasari

et al. 2021

Sci Rep

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“…Desirability calculation resulted in a value with a scale between 0-1. Desirability value of 0 represents a completely undesirable response while 1 represents the most desirable response [44]. Overall desirability in this study was 0.444 indicated several errors in the prediction model.…”

Section: Multiple Response Optimizationmentioning

confidence: 65%

Multiple Response Optimization of a HPLC Method for Analyzing Resorcinol and 4-<i>n</i>-Butyl Resorcinol in Lipid Nanoparticles

et al. 2021

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Resorcinol and 4-n-butyl resorcinol have been used to improve skin health. However, these two compounds were unstable due to the oxidation process. Lipid nanoparticle formulation strategies were reported as the solution to overcome the stability problem for both resorcinol and 4-n-butyl resorcinol. Nevertheless, it is important to determine the content of resorcinol and 4-n-butyl resorcinol in lipid nanoparticle formulation. Aiming to develop the analytical method for resorcinol and 4-n-butyl resorcinol determination, a response surface methodology (RSM) was applied in the HPLC optimization stage. An optimized HPLC condition was obtained by generating a Box-Behnken design followed by multiple response analysis. It was obtained that optimized HPLC conditions due to the predictive multiple response optimization were methanol percentage of 50.0%, acetonitrile percentage of 18.1%, and flow rate of 0.6 mL min–1. This optimized condition was successfully applied and met the requirements of the system suitability test. Quantitative estimation was performed and resulted that the resorcinol and 4-n-butyl resorcinol content in lipid nanoparticles were 70.37 ± 0.47 and 95.07 ± 0.80 µg mL–1, respectively.

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“…This combination has resulted in a new method called "Desirability Optimization Methodology or DOM" (Derringer, 1994). Desirability index is used for factor optimization in multiresponse system that is based on the transformation of all the obtained responses from different scales into a scale-free value (Amdoun et al, 2018). The values of desirability functions lie between 0 and 1.…”

Section: Formulation Codementioning

confidence: 99%

Design and optimization of candesartan loaded self-nanoemulsifying drug delivery system for improving its dissolution rate and pharmacodynamic potential

Verma

Kaushik

2020

Drug Delivery

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During the last decades, much attention has been focused on SNEDDS approach to resolve concerns of BCS II class drugs with accentuation on upgrading the solubility and bioavailability. The present hypothesis confirms the theory that SNEDDS can reduce the impact of food on Candesartan solubilization, thereby offering the potential for improved oral delivery without co-administration with meals. The present studies describe quality-by-design-based development and characterization of Candesartan loaded SNEDDS for improving its pharmacodynamic potential. D-optimal mixture design was used for systematic optimization of SNEDDS, which showed globule size of 13.91 nm, more rapid drug release rate of >90% in 30 min and 16 s for self-emulsification. The optimized formulations were extensively evaluated, where an in vitro drug release study indicated up to 1.99-and 1.10-fold enhancement in dissolution rate from SNEDDS over pure drug and marketed tablet. In vivo pharmacodynamic investigation also showed superior antihypertensive potential of SNEDDS in normalizing serum lipid levels as compared to pure drug and marketed tablet that was executed on male Wistar rats. Overall, this paper reports successful systematic development of candesartan-loaded SNEDDS with distinctly improved biopharmaceutical performance. This research work interpreted a major role of SNEDDS for enhancing the rate of dissolution and bioavailability of poorly water soluble drugs.

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The Desirability Optimization Methodology; a Tool to Predict Two Antagonist Responses in Biotechnological Systems: Case of Biomass Growth and Hyoscyamine Content in Elicited Datura starmonium Hairy Roots

Cited by 90 publications

References 39 publications

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Multiple Response Optimization of a HPLC Method for Analyzing Resorcinol and 4-<i>n</i>-Butyl Resorcinol in Lipid Nanoparticles

Design and optimization of candesartan loaded self-nanoemulsifying drug delivery system for improving its dissolution rate and pharmacodynamic potential

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