The Deletion of the Gene for the Linker Histone in<i>ARP 4</i>Mutant Yeast Cells is not Deleterious

Georgieva, Milena; Staneva, Dessislava; Uzunova, Katya; Miloshev, George

doi:10.5504/50yrtimb.2011.0025

Cited by 5 publications

(8 citation statements)

References 12 publications

(10 reference statements)

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“…The hho1delta mutant has a deleted gene for the linker histone, HHO1 [ 34 ], the arp4 mutant has a point mutation in the gene for Arp4p [ 34 ], while the double mutant arp4 hho1delta , combines the two mutations [ 35 ]. The strains were selected from our previous work [ 33 ] to allow monitoring of the chronological lifespan in different chromatin backgrounds under UVA-B stress and our previous results have shown that the three chromatin mutants have defects in their chromatin loop organization [ 26 , 35 ] and impaired, accelerated chronological aging. Moreover, to further discuss the interaction between the yeast linker histone Hho1p and Arp4p we have to point to the fact that ACT3/ARP4 is an essential gene, coding for the actin-related protein Act3p/Arp4 of Saccharomyces cerevisiae located within the nucleus.…”

Section: Resultsmentioning

confidence: 99%

“…The Mutant Yeast Cells, Lacking Functional Arp4p and the Gene for the Linker Histone, Exhibit Slower Growth Four different S. cerevisiae strains-a wild type and three chromatin mutants were cultivated under optimal and UVA/B stress conditions. The hho1delta mutant has a deleted gene for the linker histone, HHO1 [34], the arp4 mutant has a point mutation in the gene for Arp4p [34], while the double mutant arp4 hho1delta, combines the two mutations [35]. The strains were selected from our previous work [33] to allow monitoring of the chronological lifespan in different chromatin backgrounds under UVA-B stress and our previous results have shown that the three chromatin mutants have defects in their chromatin loop organization [26,35] and impaired, accelerated chronological aging.…”

Section: Resultsmentioning

confidence: 99%

“…hho1Δ (in the text appears as hho1delta )— MAT a his4-912δ-ADE2 his4-912δ lys2-128δ can1 trp1 ura3 ACT3 ypl127C::K.L.URA3 [ 34 , 35 ];…”

Section: Methodsmentioning

confidence: 99%

“…arp4ts26Δhho1 (denoted as arp4 hho1delta )— MAT a his4-912δ-ADE2 lys2-128δ can1 leu2 trp1 ura3 act3-ts26 ypl127C::K.L.URA3 [ 35 ];…”

Section: Methodsmentioning

confidence: 99%

“…Wild type (WT)-MATa his4-912δ-ADE2 his4-912δ lys2-128δ can1 trp1 ura3 ACT3 [34]; hho1∆ (in the text appears as hho1delta)-MATa his4-912δ-ADE2 his4-912δ lys2-128δ can1 trp1 ura3 ACT3 ypl127C::K.L.URA3 [34,35]; arp4ts26 (designated in the text as arp4)-MATa his4-912δ-ADE2 lys2-128δ can1 leu2 trp1 ura3 act3-ts26 [34];…”

Section: Yeast Strainsmentioning

confidence: 99%

See 4 more Smart Citations

Changes in Chromatin Organization Eradicate Cellular Stress Resilience to UVA/B Light and Induce Premature Aging

Vasileva

Staneva

Krasteva

et al. 2021

Cells

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Complex interactions among DNA and nuclear proteins maintain genome organization and stability. The nuclear proteins, particularly the histones, organize, compact, and preserve the stability of DNA, but also allow its dynamic reorganization whenever the nuclear processes require access to it. Five histone classes exist and they are evolutionarily conserved among eukaryotes. The linker histones are the fifth class and over time, their role in chromatin has been neglected. Linker histones interact with DNA and the other histones and thus sustain genome stability and nuclear organization. Saccharomyces cerevisiae is a brilliant model for studying linker histones as the gene for it is a single-copy and is non-essential. We, therefore, created a linker histone-free yeast strain using a knockout of the relevant gene and traced the way cells age chronologically. Here we present our results demonstrating that the altered chromatin dynamics during the chronological lifespan of the yeast cells with a mutation in ARP4 (the actin-related protein 4) and without the gene HHO1 for the linker histone leads to strong alterations in the gene expression profiles of a subset of genes involved in DNA repair and autophagy. The obtained results further prove that the yeast mutants have reduced survival upon UVA/B irradiation possibly due to the accelerated decompaction of chromatin and impaired proliferation. Our hypothesis posits that the higher-order chromatin structure and the interactions among chromatin proteins are crucial for the maintenance of chromatin organization during chronological aging under optimal and UVA-B stress conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…hho1Δ (in the text appears as hho1delta )— MAT a his4-912δ-ADE2 his4-912δ lys2-128δ can1 trp1 ura3 ACT3 ypl127C::K.L.URA3 [ 34 , 35 ];…”

Section: Methodsmentioning

confidence: 99%

“…arp4ts26Δhho1 (denoted as arp4 hho1delta )— MAT a his4-912δ-ADE2 lys2-128δ can1 leu2 trp1 ura3 act3-ts26 ypl127C::K.L.URA3 [ 35 ];…”

Section: Methodsmentioning

confidence: 99%

Section: Yeast Strainsmentioning

confidence: 99%

See 3 more Smart Citations

Changes in Chromatin Organization Eradicate Cellular Stress Resilience to UVA/B Light and Induce Premature Aging

Vasileva

Staneva

Krasteva

et al. 2021

Cells

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Linker histones and chromatin remodelling complexes maintain genome stability and control cellular ageing

Miloshev

Staneva

Uzunova

et al. 2019

Mechanisms of Ageing and Development

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Natural Deep Eutectic Extracts of Propolis, Sideritis scardica, and Plantago major Reveal Potential Antiageing Activity during Yeast Chronological Lifespan

Vasileva

Staneva

Grozdanova

et al. 2022

Oxidative Medicine and Cellular Longevity

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Nowadays, the environmentally friendly approach to everyday life routines including body supplementation with pharma-, nutraceuticals and dietary supplements gains popularity. This trend is implemented in pharmaceutical as well as cosmetic and antiageing industries by adopting a newly developed green chemistry approach. Following this trend, a new type of solvents has been created, called Natural Deep Eutectic Solvents (NADES), which are produced by plant primary metabolites. These solvents are becoming a much better alternative to the already established organic solvents like ethanol and ionic liquids by being nontoxic, biodegradable, and easy to make. An interesting fact about NADES is that they enhance the biological activities of the extracted biological compounds. Here, we present our results that investigate the potential antiageing effect of CiAPD14 as a NADES solvent and three plant extracts with it. The tested NADES extracts are from propolis and two well-known medicinal plants—Sideritis scardica and Plantago major. Together with the solvent, their antiageing properties have been tested during the chronological lifespan of four Saccharomyces cerevisiae yeast strains—a wild type and three chromatin mutants. The chromatin mutants have been previously proven to exhibit characteristics of premature ageing. Our results demonstrate the potential antiageing activity of these NADES extracts, which was exhibited through their ability to confer the premature ageing phenotypes in the mutant cells by ameliorating their cellular growth and cell cycle, as well as by influencing the activity of some stress-responsive genes. Moreover, we have classified their antiageing activity concerning the strength of the observed bioactivities.

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The Deletion of the Gene for the Linker Histone inARP 4Mutant Yeast Cells is not Deleterious

Abstract: Certain evidence has been collected about the imperative role of chromatin remodeling complexes (CRCs)

Cited by 5 publications

References 12 publications

Changes in Chromatin Organization Eradicate Cellular Stress Resilience to UVA/B Light and Induce Premature Aging

Changes in Chromatin Organization Eradicate Cellular Stress Resilience to UVA/B Light and Induce Premature Aging

Linker histones and chromatin remodelling complexes maintain genome stability and control cellular ageing

Natural Deep Eutectic Extracts of Propolis, Sideritis scardica, and Plantago major Reveal Potential Antiageing Activity during Yeast Chronological Lifespan

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