The degree of radiation-induced DNA strand breaks is altered by acute sleep deprivation and psychological stress and is associated with cognitive performance in humans

Moreno‐Villanueva, María; Scheven, Gudrun von; Feiveson, Alan H.; Bürkle, Alexander; Wu, Honglu; Goel, Namni

doi:10.1093/sleep/zsy067

Cited by 21 publications

(26 citation statements)

References 97 publications

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“…In order to allow DNA strand breaks repair cells were incubated at 37 °C for the indicated periods of time. DNA strand breaks were detected by the automated FADU assay [70,71], which has been successfully applied in numerous in vivo and in vitro studies [72,73,74,75,76,77,78,79,80,81,82,83,84,85]. This assay is based on alkaline DNA unwinding in a cell lysate under controlled conditions (time, pH, and temperature) staring at DNA strand breaks.…”

Section: Methodsmentioning

confidence: 99%

Influence of Acute Exercise on DNA Repair and PARP Activity before and after Irradiation in Lymphocytes from Trained and Untrained Individuals

Moreno‐Villanueva

Krämer

Hammes

et al. 2019

IJMS

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Several studies indicate that acute exercise induces DNA damage, whereas regular exercise increases DNA repair kinetics. Although the molecular mechanisms are not completely understood, the induction of endogenous reactive oxygen species (ROS) during acute exhaustive exercise due to metabolic processes might be responsible for the observed DNA damage, while an adaptive increase in antioxidant capacity due to regular physical activity seems to play an important protective role. However, the protective effect of physical activity on exogenously induced DNA damage in human immune cells has been poorly investigated. We asked the question whether individuals with a high aerobic capacity would have an enhanced response to radiation-induced DNA damage. Immune cells are highly sensitive to radiation and exercise affects lymphocyte dynamics and immune function. Therefore, we measured endogenous and radiation-induced DNA strand breaks and poly (ADP-ribose) polymerase-1 (PARP1) activity in peripheral blood mononuclear cells (PBMCs) from endurance-trained (maximum rate of oxygen consumption measured during incremental exercise V’O2max > 55 mL/min/kg) and untrained (V’O2max < 45 mL/min/kg) young healthy male volunteers before and after exhaustive exercise. Our results indicate that: (i) acute exercise induces DNA strand breaks in lymphocytes only in untrained individuals, (ii) following acute exercise, trained individuals repaired radiation-induced DNA strand breaks faster than untrained individuals, and (iii) trained subjects retained a higher level of radiation-induced PARP1 activity after acute exercise. The results of the present study indicate that increased aerobic fitness can protect immune cells against radiation-induced DNA strand breaks.

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Section: Methodsmentioning

confidence: 99%

Influence of Acute Exercise on DNA Repair and PARP Activity before and after Irradiation in Lymphocytes from Trained and Untrained Individuals

Moreno‐Villanueva

Krämer

Hammes

et al. 2019

IJMS

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“…Since its discovery, X-ray radiation has been widely used in the treatment of skin diseases. Radiation therapy uses radiation to irradiate tissue, generate secondary electrons in the body, and cause ionization, which inhibits cell division and proliferation by directly or indirectly impacting DNA strand and breaking its molecular chains [5]. Rapidly dividing and proliferating cells are sensitive to radiotherapy.…”

Section: Introductionmentioning

confidence: 99%

A Novel Radiotherapy Approach for Keloids with Intrabeam

Yang

Shao

et al. 2019

BioMed Research International

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Background. Keloids are hard nodules or plaques formed by excessive proliferation of connective tissue. Radiotherapy, widely used in various benign and malignant skin diseases, is an effective treatment for keloids. This work evaluates Intrabeam photon radiotherapy in the management of keloids. Methods. Fourteen patients who have undergone Intrabeam radiotherapy for a total of 15 sites of keloids were followed up. Twelve cases were first onset and the other two had recurrent diseases. Thirteen patients underwent surgical resection of keloids before radiotherapy. One relapsing patient received only 2 rounds of radiation therapy as she could not be reoperated. Radiotherapy was divided into 2 sessions on days 0 and 3 after surgery. The dose was 4 or 5 Gy each time for 3 min 14 s to 12 min 1 s. In addition, we compared our data to the recurrence of keloids in fourteen patients who had previously been exposed to electron beam using conventional accelerators. Results. We analyzed the treatment for adverse reactions and recurrence. In the Intrabeam group, one patient developed superficial skin ulcers a month after treatment. No one experienced wound rupture, bleeding, infection, skin contractures, or obvious hyperpigmentation. None of the fourteen cases showed any recurrence so far after on median 22.5 months of follow-up. Five patients in the electron beam group relapsed 3 to 10 months after treatment. Conclusion. Here, Intrabeam photon radiotherapy was shown to be an effective treatment for keloid scars and it is therefore recommended for management of this disease.

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“…Several approaches have been used to classify individuals as resilient or vulnerable in past research, although the optimal methods to do so remain unknown. The most common prior approaches either utilized raw performance or self-rated scores on neurobehavioral tasks during sleep deprivation [ 15 , 28 , 29 , 30 , 31 ] or utilized difference scores that accounted for baseline performance [ 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Intraindividual variance, which considers time-of-day variation in performance [ 9 , 39 , 40 , 41 , 42 , 43 , 44 ], has been posited as another potential method to characterize resilience or vulnerability to sleep deprivation.…”

Section: Interindividual Differences In Neurobehavioral Responses To Sleep Lossmentioning

confidence: 99%

“…However, further research is needed regarding this approach, since only one published study using two commonly used cognitive measures has explicitly investigated intraindividual variation as a categorization method [ 45 ]. Furthermore, the threshold used to divide individuals into resilient and vulnerable groups has also varied in prior research—studies have utilized a median split [ 28 , 29 , 30 , 32 , 35 , 36 , 46 , 47 , 48 , 49 , 50 ], a tertile split [ 15 , 34 , 38 , 51 ], a quartile split [ 31 , 52 ], or other numeric divisions of neurobehavioral performance [ 33 , 53 ]. Nevertheless, although more research is needed to determine consistent categorizations and predictors of resilience and vulnerability based on neurobehavioral performance, it remains important to explore possible biological indicators of such characteristics.…”

Section: Interindividual Differences In Neurobehavioral Responses To Sleep Lossmentioning

confidence: 99%

Genetic Markers of Differential Vulnerability to Sleep Loss in Adults

Casale

Goel

2021

Genes

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In this review, we discuss reports of genotype-dependent interindividual differences in phenotypic neurobehavioral responses to total sleep deprivation or sleep restriction. We highlight the importance of using the candidate gene approach to further elucidate differential resilience and vulnerability to sleep deprivation in humans, although we acknowledge that other omics techniques and genome-wide association studies can also offer insights into biomarkers of such vulnerability. Specifically, we discuss polymorphisms in adenosinergic genes (ADA and ADORA2A), core circadian clock genes (BHLHE41/DEC2 and PER3), genes related to cognitive development and functioning (BDNF and COMT), dopaminergic genes (DRD2 and DAT), and immune and clearance genes (AQP4, DQB1*0602, and TNFα) as potential genetic indicators of differential vulnerability to deficits induced by sleep loss. Additionally, we review the efficacy of several countermeasures for the neurobehavioral impairments induced by sleep loss, including banking sleep, recovery sleep, caffeine, and naps. The discovery of reliable, novel genetic markers of differential vulnerability to sleep loss has critical implications for future research involving predictors, countermeasures, and treatments in the field of sleep and circadian science.

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The degree of radiation-induced DNA strand breaks is altered by acute sleep deprivation and psychological stress and is associated with cognitive performance in humans

Cited by 21 publications

References 97 publications

Influence of Acute Exercise on DNA Repair and PARP Activity before and after Irradiation in Lymphocytes from Trained and Untrained Individuals

Influence of Acute Exercise on DNA Repair and PARP Activity before and after Irradiation in Lymphocytes from Trained and Untrained Individuals

A Novel Radiotherapy Approach for Keloids with Intrabeam

Genetic Markers of Differential Vulnerability to Sleep Loss in Adults

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