The deficiency in nuclear localization signal of Neodiprion lecontei nucleopolyhedrovirus DNA polymerase prevents rescue of viral DNA replication and virus production in dnapol-null Autographa californica multiple nucleopolyhedrovirus

Chen, Guoqing; Yang, Fang; Yan, Qing; Li, Pei; Wu, Lijuan

doi:10.1016/j.virusres.2019.04.005

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…The amino acid sequence consistency between NeleNPV DNApol and AcMNPV DNApol is only 26%. Some motifs important for nuclear localization and viral replication are not found in the NeleNPV DNApol [65], indicating that AcMNPV and its close relatives have evolved a more flexible NLS system to improve the efficiency of nuclear transport. NLSs have also been detected on the DNApol of the beta baculovirus PiraGV.…”

Section: Summary and Future Prospectsmentioning

confidence: 99%

Molecular Mechanisms of Nuclear Import of Baculovirus Proteins

Li,

Wang,

2024

Preprint

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Baculovirus is a type of virus with a double stranded, circular, and super helical DNA structure, whose transcription, replication and assembly of offspring virus occur in the nucleus of the cell. However, due to the natural barrier of the cellular nuclear membrane, macromolecular substances larger than 40-60 kDa cannot directly penetrate the nuclear membrane. Therefore, baculovirus have evolved various strategies to achieve transmembrane transport of viral proteins. This article attempts to review the recent literature on mechanisms by which the DNA baculoviruses of insects transport proteins into the infected host cell nucleus. In this article, we summarise the studies of nuclear localisation signals(NLSs) in baculovirus, which are involved in the classical nuclear import pathways and review the non-classical nuclear transport pathways independent on NLS derived from baculovirus. Moreover, during the protein nuclear transport process, viruses will regulate the nuclear pathway through protein modifications. Host structure changes and other methods will create a favorable environment for the nuclear transport of viral proteins.

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Section: Summary and Future Prospectsmentioning

confidence: 99%

Molecular Mechanisms of Nuclear Import of Baculovirus Proteins

Li,

Wang,

2024

Preprint

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Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses

DOĞAN,

BUDAK,

SALMAN

et al. 2023

Preprint

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The complete genome of the European pine sawfly Neodiprion sertifer nucleopolyhedrovirus was sequenced and characterized from next-generation sequencing data of the N. sertifer larva from Turkiye. This genome was comparatively analysed by previously reported genomes baculoviruses. The baculovirus phylogeny was reconstructed and the species boundary of the NeseNPV-TR was delineated using K2P distance. The length of the genome was 82,052 bp, with a G + C content of 33.28%. It contained 82 putative ORFs, including 33 baculovirus core genes, three lepidopteran baculovirus core genes, three non-conserved genes. It had five hrs with 20.6% overall mean distance on average. The pairwise K2P distances of lef-9 and polh genes were lower than the specified threshold value, while those of lef-8, combinations of three genes and 33 genes were slightly higher between NeseNPV-TR and NeseNPV. The most variable genes were lef-2, helicase, p40, desmoplakin, p6.9, vp91 and vp39, while the most conserved were lef-8, lef-9, odv-e18, pif2 and lef-5 among baculoviruses. The genome of NeseNPV-TR is smaller and contains the fewest ORFs among baculoviruses. Some of unassigned ORFs had conserved domains and hence, we suggest further investigation to determine their structural and functional roles. Phylogenetic analyses confirmed its position within Gammabaculovirus. The NeseNPV-TR can be considered as the same species with NeseNPV. The different divergence rates in the baculovirus core genes may be related with different selection pressures acting on the genes. The lower genetic diversity of Group I alphabaculoviruses is most probably due to recent emergence.

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A Comprehensive Comparative Analysis on the Codon Usage Bias of DNA Polymerase Genes in Invertebrate Iridescent Viruses

Aktürk Dizman

2024

Recep Tayyip Erdoğan Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi

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Invertebrate iridescent viruses (IIVs) are classified as double-stranded DNA viruses within the Iridoviridae family. IIVs are viruses that infect invertebrate hosts, causing symptoms that vary in intensity from slight reductions in host fitness to systemic disease. Numerous earlier investigations have provided insights into the genomic, proteomic, and transcriptional analyses of invertebrate iridescent viruses. However, the codon usage bias (CUB) of IIVs has yet to be fully understood. In order to gain a more profound insight into the evolutionary features of IIVs, we conducted an extensive analysis of the codon usage patterns in the DNA polymerase genes (DNA pol genes) of 12 invertebrate iridescent viruses. The analysis of both nucleotide composition and relative synonymous codon usage (RSCU) indicated a higher prevalence of AT-ended codons in the DNA pol genes of IIVs. Additionally, a low codon usage bias was determined from the effective number of codons (ENC) value. Analyses of ENC-GC3s plot, neutrality plot, and parity rule 2 plot illustrated that the codon usage patterns in IIVs DNA pol genes were influenced by both natural selection and mutational pressure. This investigation holds significance as it has delineated the codon usage patterns within the DNA pol genes of IIVs and has furnished crucial data for a foundational study of their evolutionary aspects.

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The deficiency in nuclear localization signal of Neodiprion lecontei nucleopolyhedrovirus DNA polymerase prevents rescue of viral DNA replication and virus production in dnapol-null Autographa californica multiple nucleopolyhedrovirus

Cited by 3 publications

References 21 publications

Molecular Mechanisms of Nuclear Import of Baculovirus Proteins

Molecular Mechanisms of Nuclear Import of Baculovirus Proteins

Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses

A Comprehensive Comparative Analysis on the Codon Usage Bias of DNA Polymerase Genes in Invertebrate Iridescent Viruses

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