The defH9-iaaM auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberry

Mezzetti, Bruno; Landi, L.; Pandolfini, Tiziana; Spena, Angelo

doi:10.1186/1472-6750-4-4

Cited by 121 publications

(30 citation statements)

References 17 publications

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“…Not consistent with other genes causing swollen and misshapen parthenocarpic fruit or changing the structure of floral organs (Bruno et al 2004;Charles et al 2002), 2A12-iaaM parthenocarpic fruits of transgenic P. pubescens had a shape similar to that of the control and no malformations were observed. The green color of the pericarp that was observed in parthenocarpic fruits grown in greenhouse conditions might be due to the increased IAA content caused by the iaaM gene.…”

Section: Iaa Analysis and Phenotype Of Transgenic Plantsmentioning

confidence: 81%

“…Flowers and fruits collected at the same developmental stages were analyzed. Gas chromatography-mass spectrometry of the tissue total IAA content was performed as described by Mezzetti et al (2004). This value was expressed in picomoles per gram of tissue (fresh weight) and included both free and conjugated IAA.…”

Section: Iaa Analysismentioning

confidence: 99%

“…To fully exploit the advantages offered by parthenocarpy, several methods of genetic engineering of parthenocarpic fruit development have been proposed. For instance, the chimeric gene DefH9-iaaM, which could promote the synthesis of IAA (Pandolfini et al 2002;Molesini et al 2009), has been used to confer parthenocarpic fruit development in several species belonging to different plant families (Mezzetti et al 2004), and the agronomical advantages of DefH9-iaaM plants have been assessed in greenhouse and field trials (Yin et al 2006;Giuseppe et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Wang

Xin

Qin

et al. 2011

Plant Cell Tiss Organ Cult

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An efficient somatic embryogenesis system for Physalis pubescens L. (husk tomato) was developed prior to transformation. Subsequently, cotyledonary explants of P. pubescens were transformed with a chimeric construct containing an iaaM gene from driven by the fruit-specific promoter 2A12 to develop parthenocarpic fruits. Following selection of explants on Murashige and Skoog (MS) medium containing containing 75 mg l -1 kanamycin (Km), 36 km-resistant callus clusters were recovered, and these were regenerated into whole plants. Expression of the iaaM gene was detected in confirmed transgenic fruits. The 0.9-kb 2A12 promoter was capable of directing expression of the introduced iaaM gene in transgenic P. pubescens fruits, but iaaM expression was absent from both leaves and flowers. Quantitative measurements of indole-3-acetic acid (IAA) content during fruit development indicated that the IAA levels in transgenic lines increased from anthesis through young fruits and peaked at fruit maturity. On average, IAA contents in transgenic fruits were two-fold higher than those in control fruits. Under greenhouse condition, vegetative growth, morphology, and the flowering of transgenic plants were comparable to those of control plants. However, the fruits of transgenic lines ripened earlier and had fewer seeds per fruit than did control plants.

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Section: Iaa Analysis and Phenotype Of Transgenic Plantsmentioning

confidence: 81%

Section: Iaa Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Wang

Xin

Qin

et al. 2011

Plant Cell Tiss Organ Cult

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“…We applied the Delta-DeltaCt comparative method for quantification (Ingham et al 2001), using as external references two Fragaria x ananassa octoploid transgenic strawberry lines with one and three copies of the nptII and DefH9-iaaM genes, respectively, analyzed by Southern blotting (Mezzetti et al 2004b). The copy numbers in the 'rolC Calypso' lines were determined according to the equation: 10 ðC t referenceÀC t GM targetÞ slope where C t is the threshold cycle and reflects the cycle number at which the fluorescence generated within the reaction for every sample crosses the threshold; the calibrator is a sample used as the basis for comparative results; the transgenes target is the 'Calypso rolC' lines and the slope is calculated according to the standard curve, which is specific for each gene, and which reflects the amplification efficiency.…”

Section: Plant Materialsmentioning

confidence: 99%

ROLC strawberry plant adaptability, productivity, and tolerance to soil-borne disease and mycorrhizal interactions

et al. 2009

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The potential to improve strawberry cultivation was assessed regarding the use the rolC genes from Agrobacterium rhizogenes that can confer higher levels of free cytokinins. Strawberry (cv. Calypso) rolC lines were produced by genetic transformation of Agrobacterium tumefaciens. Yield and fruit quality of the control and transgenic lines were measured under open-field conditions. The effects of the transgenic rolC lines depended on gene copy number: rolC lines with one (Line A) or two gene (Line B) copies showed 30% greater yields than controls, due to 20% more fruit per plant and an increased fruit weight. Line A also differed in terms of the highest fruit quality, due to 10.5% increased soluble solids and 12.7% higher acidity. Moreover, cv. Calypso rolC lines A and B had increased tolerance to greenhouse infection by Phytophthora cactorum. Conversely, for all of these characters, Line F (five rolC copies) was not significantly different from the control line. The same lines were also used to examine their symbiosis with root arbuscular mycorrhizal fungi (AMF) using vital and non-vital staining of roots collected at different stages of plant growth. Control and rolC plants showed similar intensities of AMF infection according to plant phenology and/or physiology. Furthermore, possible horizontal gene transfer of the rolC gene was tested for the AMF spores by PCR, with all AMF samples negative using rolC primers. The use of the rolC gene should be considered for the improvements provided in productivity, fruit quality and disease resistance of cultivated strawberry that show no effects on soil microorganisms.

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“…A number of genetically transformed lines of Fragaria sp. were obtained throughout genetic engineering to study gene functions or to improve traits such as resistance, ripening, and fruit production (Haymes and Davis, 1998; Mezzetti et al, 2004; Schaart et al, 2004, 2013; Oosumi et al, 2006; Jiwan et al, 2013; Pantazis et al, 2013; see also reviews: Folta and Davis, 2006; Aldwinckle and Malnoy, 2009). Indeed, in strawberry the efficiency of transformation and the regeneration capacity is highly dependent on the genetic background (Landi and Mezzetti, 2006) and several critical factors such as hormones concentrations, incubation conditions, explant source, antibiotic, and mode of transfections can affect the frequency of regeneration (Folta and Dhingra, 2006).…”

Section: The Strawberry Fruit Casementioning

confidence: 99%

Transient transformation meets gene function discovery: the strawberry fruit case

Guidarelli

Baraldi

2015

Front. Plant Sci.

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Beside the well known nutritional and health benefits, strawberry (Fragaria X ananassa) crop draws increasing attention as plant model system for the Rosaceae family, due to the short generation time, the rapid in vitro regeneration, and to the availability of the genome sequence of F. X ananassa and F. vesca species. In the last years, the use of high-throughput sequence technologies provided large amounts of molecular information on the genes possibly related to several biological processes of this crop. Nevertheless, the function of most genes or gene products is still poorly understood and needs investigation. Transient transformation technology provides a powerful tool to study gene function in vivo, avoiding difficult drawbacks that typically affect the stable transformation protocols, such as transformation efficiency, transformants selection, and regeneration. In this review we provide an overview of the use of transient expression in the investigation of the function of genes important for strawberry fruit development, defense and nutritional properties. The technical aspects related to an efficient use of this technique are described, and the possible impact and application in strawberry crop improvement are discussed.

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The defH9-iaaM auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberry

Cited by 121 publications

References 17 publications

Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

ROLC strawberry plant adaptability, productivity, and tolerance to soil-borne disease and mycorrhizal interactions

Transient transformation meets gene function discovery: the strawberry fruit case

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