The deduced primary structure of a ribosomal protein S4 from Trypanosoma cruzi

Proteomics in Trypanosoma cruzi – localization of novel proteins to various organelles

“…In agreement with the enrichment of organelles in this fraction (Fig. 3B), we found well established markers of T. cruzi acidocalcisomes (vacuolartype proton translocating pyrophosphatase, EAN91609.1) [21], vacuolar ATPase, subunit C, EAN86373.1 [32], and vacuolar-type Ca 1 ATPase (EAN95492.1) [32]; glycosomes (solanesyl diphosphate synthase, EAN80692.1 [33], hexokinase, EAN94612.1 [34], pyruvate phosphate dikinase, EAN86096.1 [35], phosphoenolpyruvate carboxykinase, EAN88964.1 [36], and malate dehydrogenase, EAN90616.1 [37]); mitochondrion (glutamate dehydrogenase, EAN87724.1 [38], aspartate aminotransferase, EAN86462.1 [39], alanine aminotransferase, EAO00085.1 [39], malate dehydrogenase, EAN87359.1 [37], and kinetoplast DNAassociated protein, EAN85210.1 [40]); reservosomes (GST, EAN88371.1 [41], cysteine peptidase, EAN83138.1 [42], and serine carboxypeptidase, EAN81557.1 [43]); ER (40S ribosomal protein S4, EAN87845.1 [44], and calreticulin, EAN82340.1 [45]); and flagellum and cytoskeletal components (paraflagellar rod proteins, EAN92318.1, EAN87979.1, EAN81200.1, EAN83974.1, EAN99876.1 [46], flagellar calcium-binding protein, EAN86963.1 [47], b, EAN94839.1), and a (EAN81053.1) tubulins [48], actin, EAN85411.1 [49], and kinetoplastid membrane protein KMP-11, EAN87014.1 [50]). Among the only five proteins that were known to localize exclusively to the plasma membrane we detected two that belong to families with large numbers of members (mucin-associate surface protein, EAN84550.1 [14], and trans-sialidase EAN86366.1 [51]).…”

Trypanosoma cruzi ribosomal protein S4: characterization of its coding locus, analysis of transcripts, and antigenicity of the protein

“…The library was screened using the EcoR I -Kpn I rpS4 encoding fragment from the T. cruzi I (Tulahuen strain) cDNA clone pS4-2 (Hernandez et al 1998). Five positive clones were identified which contained two different inserts 8.5 and 7.5 Kb in size.…”

The stabilization of housekeeping transcripts inTrypanosoma cruziepimastigotes evidences a global regulation of RNA decay during stationary phase

“…To prepare gene probes, DNA fragments were gel purified from the following T. cruzi plasmid clones: pD‐4, actin genomic clone [5]; pBTR, plasmid bearing a PCR derived coding sequences from the tripanothion reductase (TR) gene [6]; recombinant plasmid composed the pCR II vector carrying a genomic derived PCR amplification product from the triosephosphate isomerase (TIM) gene [7]; pS4–2, cDNA clone from the ribosomal protein S4 (S4) locus [8]. In northern blot analysis of total RNA obtained from epimastigotes in culture, each of these probes recognizes a single mRNA band at every stage during growth (data not shown).…”