The decellularized ovary as a potential scaffold for maturation of preantral ovarian follicles of prepubertal mice

Alaee, Sanaz; Asadollahpour, Raheleh; Colagar, Abasalt Hosseinzadeh; Talaei‐Khozani, Tahereh

doi:10.1080/19396368.2021.1968542

Cited by 12 publications

(11 citation statements)

References 61 publications

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“…Seven studies used detergent combinations ( Nikniaz et al , 2021 ; Pennarossa et al , 2021 ; Sarabadani et al , 2021 ; Chiti et al , 2022 ; Fazelian-Dehkordi et al , 2022 ; Zheng et al , 2022 ; Wu et al , 2022b ). From the perspective of chemical types, ionic detergents (SDS, sodium deoxycholate, SLES) were the most commonly used reagents (n = 10) ( Laronda et al , 2015 ; Hassanpour et al , 2018 ; Alaee et al , 2021 ; Nikniaz et al , 2021 ; Pennarossa et al , 2021 ; Chiti et al , 2022 ; Haghshenas et al , 2022 ; Zheng et al , 2022 ; Wu et al , 2022b ), and five studies used non-ionic detergents, mainly Triton X-100 ( Nikniaz et al , 2021 ; Pennarossa et al , 2021 ; Chiti et al , 2022 ; Zheng et al , 2022 ; Wu et al , 2022b ). Less frequently used detergents included ammonium hydroxide and 2-propanol.…”

Section: Resultsmentioning

confidence: 99%

Extracellular matrix-derived scaffolds in constructing artificial ovaries for ovarian failure: a systematic methodological review

Huang

Yan

et al. 2023

Human Reproduction Open

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STUDY QUESTION What is the current state-of-the-art methodology assessing decellularized extracellular matrix (dECM)-based artificial ovaries for treating ovarian failure? SUMMARY ANSWER Preclinical studies have demonstrated that decellularized scaffolds support the growth of ovarian somatic cells and follicles both in vitro and in vivo. WHAT IS KNOWN ALREADY Artificial ovaries are a promising approach for rescuing ovarian function. Decellularization has been applied in bioengineering female reproductive tract tissues. However, decellularization targeting the ovary lacks a comprehensive and in-depth understanding. STUDY DESIGN, SIZE, DURATION PubMed, Embase, Web of Science and the Cochrane Central Register of Controlled Trials were searched from inception until October 20, 2022 to systematically review all studies in which artificial ovaries were constructed using decellularized extracellular matrix scaffolds. The review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol. PARTICIPANTS/MATERIALS, SETTING, METHODS Two authors selected studies independently based on the eligibility criteria. Studies were included if decellularized scaffolds, regardless of their species origin, were seeded with ovarian cells or follicles. Review articles and meeting papers were removed from the search results, as were articles without decellularized scaffolds or recellularization or decellularization protocols, or control groups or ovarian cells. MAIN RESULTS AND THE ROLE OF CHANCE The search returned a total of 754 publications, and 12 papers were eligible for final analysis. The papers were published between 2015 and 2022, and were most frequently reported as coming from Iran. Detailed information on the decellularization procedure, evaluation method and preclinical study design was extracted. In particular, we concentrated on the type and duration of detergent reagent, DNA and extracellular matrix detection methods, and the main findings on ovarian function. Decellularized tissues derived from humans and experimental animals were reported. Scaffolds loaded with ovarian cells have produced estrogen and progesterone, though with high variability, and have supported the growth of various follicles. Serious complications have not been reported. LIMITATIONS, REASONS FOR CAUTION A meta-analysis could not be performed. Therefore, only data pooling was conducted. Additionally, the quality of some studies was limited mainly due to incomplete description of methods, which impeded specific data extraction and quality analysis. Several studies that used dECM scaffolds were performed or authored by the same research group with a few modifications, which might have biased our evaluation. WIDER IMPLICATIONS OF THE FINDINGS Overall, the decellularization-based artificial ovary is a promising but experimental choice for substituting insufficient ovaries. A generic and comparable standard should be established for the decellularization protocols, quality implementation, and cytotoxicity controls. Currently, decellularized materials are far from being clinically applicable to artificial ovaries. STUDY FUNDING/COMPETING INTEREST(S) This study was funded by the National Natural Science Foundation of China (Nos. 82001498 and 81701438). The authors have no conflicts of interest to declare. TRIAL REGISTRATION NUMBER This systematic review is registered with the International Prospective Register of Systematic Reviews (PROSPERO, ID CRD42022338449).

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Section: Resultsmentioning

confidence: 99%

Extracellular matrix-derived scaffolds in constructing artificial ovaries for ovarian failure: a systematic methodological review

Huang

Yan

et al. 2023

Human Reproduction Open

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“…Other research groups have also used combined methods, among which SDS, Triton X-100, and SDC were the most frequently used [ 15 , 19 ]. Other reagents such as sodium lauryl ester sulfate, NaOH, and propanol-2 were also used to decellularize ovaries [ 12 , 20 , 21 ]. However, comparisons of the efficiency and cytotoxicity are limited.…”

Section: Discussionmentioning

confidence: 99%

Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice

Gao

Tang

et al. 2022

JFB

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Substitution by artificial ovary is a promising approach to restore ovarian function, and a decellularized extracellular matrix can be used as a supporting scaffold. However, biomimetic ovary fabrication and immunogenicity requires more investigation. In this study, we proposed an effective decellularization protocol to prepare ovarian scaffolds, which were characterized by few nuclear substances and which retained the extracellular matrix proteins. The ovarian tissue shape and 3-dimensional structure were well-preserved after decellularization. Electron micrographs demonstrated that the extracellular matrix fibers in the decellularized group had similar porosity and structure to those of native ovaries. Semi-quantification analysis confirmed that the amount of extracellular matrix proteins was reduced, but the collagen fiber length, width, and straightness did not change significantly. Granulosa cells were attached and penetrated into the decellularized scaffold and exhibited high proliferative activity with no visible apoptotic cells on day 15. Follicle growth was compromised on day 7. The implanted artificial ovaries did not restore endocrine function in ovariectomized mice. The grafts were infiltrated with immune cells within 3 days, which damaged the artificial ovary morphology. The findings suggest that immune rejection plays an important role when using artificial ovaries.

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“… Hossein Nikniaz [ 190 ] 2021 Human and bovine acellular ovarian scaffold Mouse preantral follicles Sodium alginate containing acellular ovarian scaffold could maintain follicular viability in vitro. Sanaz Alaee[ 191 ] 2021 Decellularized rat ovarian scaffold Preantral follicles from prepubertal mice The preantral follicles transformed into antral follicles, and produced mature meiosis oocytes. Wen-Yue Liu[ 187 ] 2017 Porcine acellular scaffold Rat ovarian tissue Supported the adhesion, migration, and proliferation of immature female rat granulose cells and showed estradiol secretion.…”

Section: Biomaterials For Ovarian Aging Therapymentioning

confidence: 99%

“…Nikniaz et al cultured isolated mouse preantral follicles into an acellular ovarian scaffold and tested the survival rate of follicles for the first time, demonstrating that sodium alginate-containing acellular ovarian scaffolds could maintain follicular viability in vitro for 6 days [ 190 ]. Similarly, Alaee et al cultured preantral follicles from mice in a decellularized rat ovarian scaffold for 12 days [ 191 ]. In the acellular ovarian scaffold, the preantral follicles transformed into antral follicles, and the mature follicles secreted E2 and progesterone (P4), and they could grow and develop normally and produce mature meiosis oocytes.…”

Section: Biomaterials For Ovarian Aging Therapymentioning

confidence: 99%

See 1 more Smart Citation

Biomaterials and advanced technologies for the evaluation and treatment of ovarian aging

Guo

Wei

et al. 2022

J Nanobiotechnol

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Ovarian aging is characterized by a progressive decline in ovarian function. With the increase in life expectancy worldwide, ovarian aging has gradually become a key health problem among women. Over the years, various strategies have been developed to preserve fertility in women, while there are currently no clinical treatments to delay ovarian aging. Recently, advances in biomaterials and technologies, such as three-dimensional (3D) printing and microfluidics for the encapsulation of follicles and nanoparticles as delivery systems for drugs, have shown potential to be translational strategies for ovarian aging. This review introduces the research progress on the mechanisms underlying ovarian aging, and summarizes the current state of biomaterials in the evaluation and treatment of ovarian aging, including safety, potential applications, future directions and difficulties in translation. Graphical Abstract

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The decellularized ovary as a potential scaffold for maturation of preantral ovarian follicles of prepubertal mice

Cited by 12 publications

References 61 publications

Extracellular matrix-derived scaffolds in constructing artificial ovaries for ovarian failure: a systematic methodological review

Extracellular matrix-derived scaffolds in constructing artificial ovaries for ovarian failure: a systematic methodological review

Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice

Biomaterials and advanced technologies for the evaluation and treatment of ovarian aging

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