The Cytotoxic Response to Murine Cytomegalovirus. I. Parameters in vivo

Delayed-type Hypersensitivity Responses to Murine Cytomegalovirus in Genetically Resistant and Susceptible Strains of Mice

“…Cytotoxic T cells generated in vivo during MCMV infection are specific for cells that express immediate early proteins in assays in vitro (Reddehase & Kosinowski, 1984;Reddehase et al, 1984a, b). However, the primary cytotoxic response in the animal cannot be easily detected by assays in vitro and requires a further step of incubation of lymphocytes from infected mice with IL-2 in vitro (Reddehase & Koszinowski, 1984;Sinickas et al, 1985a, b). This observation raises uncertainty concerning the usefulness of assays in vitro for the detection of cytotoxic T cells involved in immunity against herpesviruses.…”

“…The infection of mice with murine cytomegalovirus (MCMV) elicits the production of T cells which proliferate (Howard et al, 1978) or produce lymphokines (Sinickas et al, 1985 c) following re-exposure to MCMV in vitro ; they lyse virus-infected target cells in vitro (Ho, 1980;Reddehase & Koszinowski, 1984;Sinickas et al, 1985a, b) or mount delayed-type hypersensitivity (DTH) responses in vivo (Chong & Mires, 1982). Although the relative importance of T cell-mediated responses compared with other host protective mechanisms remains to be determined, T cells are important in regulating the course of primary MCMV infection since nude mice show 1" Present address: Department of Virology, Royal Free Hospital, Hampstead, London, U.K.…”

The cytotoxic response to murine cytomegalovirus: requirements for the generation of MCMV‐specific target cells

“…Mouse embryo fibroblasts and the contitiuous cell line BALB/C-3T3 were cultured as previously described (4). The DBA/2 masiocyioma cell line P-815-X2 (P815) was cultured in DMEM at 37" in special gas atmosphere.…”

“…Slandard meihods were used for ihc preparation of salivary gland stock of the Smith strain of MCMV (4). Tissue culture-passaged Smith strain MCMV was prepared by modificaiion of a described meihod (6), Briefly, secondary passage mouse embryo fibroblasts (MHF) were seeded into 30 cm-plastic peiri dishes {Cai.…”

“…As an approach to the immunobiology of viruses that cause latent infections, the T ceil response lo MCMV was investigated. Previous reports (4,5) showed that hind footpad inoculation of mice with MCMV, removal of popliteal lymph node (LN) cells at day 7 and subsequent culture of these LN cells for 4 days was a reliable method for the generation of MCMVimmune T cells. One functional characteristic of this immune T cell population is that they are cytotoxic for MCMV-infected, H-2-matched target cells (4, 5).…”