The cytotoxic response to murine cytomegalovirus: requirements for the generation of MCMV‐specific target cells

Abstract

Summary. The preparation of target celts susceptible to lysis by murine c>'tomegalovirus (MCMV)-immune T cells in vitro was investigated and found to be dependeni upon target cell type, culture conditions, virus adsorption proiocol and virus preparation. Optimally sensitive MCMV-infected targeis were obtained by preculturc of mouse embryo fibroblasts (Mth) in 3"/o vol/vol concanavalin-A-stimu!ated spleen cell supernatant (CSS)-supplemenied medium, adsorption of salivary gland stock MCMV under 800 g centrifugation atid at least 4 h furlher incubation at 37' before addition of T cells. In conirast, salivary gland stock MCMV did tiot cau,se thioglycollate-induced peritoneal exudaic cells lo be susceptible to MCMVspecific T cell-mediated lysis, whereas tissue culiure-passaged stock MCMV was successful. The preparation of MCMV-infecied target cells is discussed in terms of the need for H-2 and viral antigen expression for T cell recognition.

Quantitative Considerations of T‐Cell Activation and Self Tolerance

“…They showed that gamma-interferon induced a 5-fold increase in ST-4.5 target cell expression of H-2K'', and that for 6 out of a panel of 15 K''-reactive Tc clones this caused > 30-foId reduction in the effector: target ratio causing 50% lysis. Sinickas et al (1987) have produced similar evidence using lymphokine release from class I H-2 alloreactive T-cell populations (Fig. 4).…”

Thymic Selection Reinterpreted

“…Shimonkewitz et al (1985), for example, have shown that >'-IFN induces a 5-foId increase in target cell expression of H-2K'', and that for 6 of a panel of 15 K''-reactive T-cell clones this caused a greater than 30-fold reduction in the effector/target ratio causing 50% lysis. Similarly, Sinickas et al (1987) demonstrated that y-IFN-treated mouse embryo fibroblasts were approximately 10 times more efficient than untreated cells at inducing lL-3 release from alloreactive T cells. Both effects are likely in large part to reflect the requirement of T cells for multivalent interaction between TCRs and MHC/peptide (Herrmann & Mescher 1986).…”