The Cytoplasmic Loops of Subunit a of Escherichia coli ATP Synthase May Participate in the Proton Translocating Mechanism

Moore, Kyle J.; Angevine, Christine M.; Vincent, Owen D.; Schwem, Brian; Fillingame, Robert H.

doi:10.1074/jbc.m800900200

Cited by 34 publications

(45 citation statements)

References 53 publications

(60 reference statements)

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“…Further, after introduction of pairs of Cys into the two loop regions, cross-linking between the Ag + -sensitive loop regions was demonstrated (27). The latter result suggested these regions of cytoplasmic loops might pack into a single domain that would gate H + translocation from the middle of the membrane to the cytoplasm.…”

Section: Cross-linking Of H + Transporting Regions In Loops Of Subunimentioning

confidence: 69%

“…Passive H + transport by aK203C liposomes was not as sensitive to Cd +2 , and Cd +2 may inhibit ATP-driven H + transport in this mutant by additional mechanisms. Ag + -sensitive Cys in the 1-2 loop had previously been shown to cross-link with Ag + -sensitive Cys in the 3-4 loop and suggested that the possible H + -transporting regions might interact structurally in a single gating domain (27) (Fig. 7).…”

Section: Discussionmentioning

confidence: 99%

“…Further, on the basis of the previous cross-linking experiments described by Moore and colleagues (27), we suggest that the hypothesized gating region of the 3-4 loop, centered in the region of the aS199C and aS202C substitutions, also packs within a single domain gating H + release to the cytoplasm (Discussion).…”

Section: Cross-linking Of H + Transporting Regions In Loops Of Subunimentioning

confidence: 99%

“…Subunit c. Moore and colleagues (27) were the first to identify subunit a Cys substitutions in both the 1-2 loop (residues 86 and 93) and the 3-4 loop (residues 177,195,198,201,202, and 203), in which ATP-driven H + transport was blocked by Ag + treatment. Further, after introduction of pairs of Cys into the two loop regions, cross-linking between the Ag + -sensitive loop regions was demonstrated (27).…”

Section: Cross-linking Of H + Transporting Regions In Loops Of Subunimentioning

confidence: 99%

“…We have also reported Ag + -sensitive Cys substitutions in two cytoplasmic loops of subunit a (27) and, more recently, in the cytoplasmic loop of subunit c (28). The mechanism by which Ag + inhibited F 1 F 0 -mediated H + transport was uncertain.…”

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confidence: 99%

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Interacting cytoplasmic loops of subunits a and c of Escherichia coli F ₁ F ₀ ATP synthase gate H ⁺ transport to the cytoplasm

Steed

Kraft

Fillingame

2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance H + transport through the membrane-traversing F 1 F 0 ATP synthase mechanically drives bond formation between ATP and P i . For the enzyme in the inner membrane of Escherichia coli , we show here that the H + transport pathway extends beyond the lipid bilayer into cytoplasmic loops connecting transmembrane helices in both subunits a and c of the transmembrane F 0 sector. On the basis of chemical cross-linking, we postulate that the multiple loop regions functioning in H + transport pack into a single domain, which then interacts with the cytoplasmic surface of the H + channel within F 0 . The interaction of the loop regions with the H + channel is proposed to specifically gate H + release to the cytoplasmic side of the membrane during ATP synthesis.

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Section: Cross-linking Of H + Transporting Regions In Loops Of Subunimentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Cross-linking Of H + Transporting Regions In Loops Of Subunimentioning

confidence: 99%

Section: Cross-linking Of H + Transporting Regions In Loops Of Subunimentioning

confidence: 99%

mentioning

confidence: 99%

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Interacting cytoplasmic loops of subunits a and c of Escherichia coli F ₁ F ₀ ATP synthase gate H ⁺ transport to the cytoplasm

Steed

Kraft

Fillingame

2014

Proc. Natl. Acad. Sci. U.S.A.

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Silver nanoparticles‐modified films versus biomedical device‐associated infections

Cao

Liu

2010

WIREs Nanomed Nanobiotechnol

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A serious issue related to biomedical devices (BDs) is that of bacterial infections. BDs colonized by bacteria may cause infection or mortality. To prevent such infections, an effective strategy is to develop novel BDs with antibacterial abilities via various surface modification processes. Thus, plenty of silver nanoparticles (Ag NPs)-modified films were brought forward to because of their potential applications in improving the antibacterial properties of BDs. This article reviews the difficulties in diagnosing and treating biomedical device-associated infections as well as the state of arts in fabricating the Ag NPs-modified films for antibacterial applications. In addition, the nanoeffect of silver particles and the cytotoxicity of Ag NPs are also discussed. It is clear that safe and durable Ag NPs-modified films are more desirable for the BDs prone to bacteria. To further extend the investigations on controlling the toxicity path of Ag NPs to both bacteria and mammalian cells, developing novel green fabrication processes with more 'cleaner' (without accompaniment of ligands or reduction agents) Ag NPs should be the first mission for the material scientists to complete.

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Energy Transduction by the Two Molecular Motors of the F1Fo ATP Synthase

et al. 2011

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The Cytoplasmic Loops of Subunit a of Escherichia coli ATP Synthase May Participate in the Proton Translocating Mechanism

Cited by 34 publications

References 53 publications

Interacting cytoplasmic loops of subunits a and c of Escherichia coli F ₁ F ₀ ATP synthase gate H ⁺ transport to the cytoplasm

Interacting cytoplasmic loops of subunits a and c of Escherichia coli F ₁ F ₀ ATP synthase gate H ⁺ transport to the cytoplasm

Silver nanoparticles‐modified films versus biomedical device‐associated infections

Energy Transduction by the Two Molecular Motors of the F1Fo ATP Synthase

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The Cytoplasmic Loops of Subunit a of Escherichia coli ATP Synthase May Participate in the Proton Translocating Mechanism

Cited by 34 publications

References 53 publications

Interacting cytoplasmic loops of subunits a and c of Escherichia coli F 1 F 0 ATP synthase gate H + transport to the cytoplasm

Interacting cytoplasmic loops of subunits a and c of Escherichia coli F 1 F 0 ATP synthase gate H + transport to the cytoplasm

Silver nanoparticles‐modified films versus biomedical device‐associated infections

Energy Transduction by the Two Molecular Motors of the F1Fo ATP Synthase

Contact Info

Product

Resources

About

Interacting cytoplasmic loops of subunits a and c of Escherichia coli F ₁ F ₀ ATP synthase gate H ⁺ transport to the cytoplasm

Interacting cytoplasmic loops of subunits a and c of Escherichia coli F ₁ F ₀ ATP synthase gate H ⁺ transport to the cytoplasm