The cytoplasmic distribution and characterization of poly(A)+ RNA in oocytes and embryos of Drosophila

Lovett, Janice; Goldstein, Elliott S.

doi:10.1016/0012-1606(77)90342-6

Cited by 59 publications

(14 citation statements)

References 41 publications

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“…By performing dot hybridizations with Dare, ar.14, or Dpuh probes, the fraction of the total hybridizable RNA detected by each probe that was preasent in the polyribosome region of the gradient was determined (Table 1). Between 40 and 60% of the IW_qQ-family RNA sequenoes were present on polyribosomes; very similar to the fraction of total poly(A)-containing RNA present on Droaoi±UA embryo polyribosomes (27). nitrocoellulose and hybridized with Dsrc, _4, and Dah probes (Fig.…”

Section: Screening the Bacterionhage Lambda Drosonhila Geoe Librarmentioning

confidence: 60%

Maternal inheritance of transcripts from threeDrosophila src-related genes

Wadsworth¹,

Madhavan

Bilodeau-Wentworth³

1985

Nucl Acids Res

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SU1MHARYThe =r2&pbhUj genome contains three major sequenoes related to the v-.A gene. Previously published molecular studies have confirmed the structural homology between v-Ar and two of the Droskph1aa sequences. We have sequenced a portion of the third v-gUM-related DroaolhUA gene and found that it also shares structural homology with vertebrate and Drosowb±A =-family genes. RNA sequences from each of the = genes are present in pre-blastoderm embryos indicating that they are of maternal origin. As embryogenesis prooeeds, the levels of each of the are RNA sequences decline. The pre-blastoderm = gene transcripts contain poly(A) and are present on polyribosomes suggesting that they are functional mRKAs. Sinoe the DrkQgRW.a Arg transcripts were maternally inherited, we also investigated their distribution in adult females. The majority of the a transcripts in adult females were contained in ovaries. Only low levels of the transcripts were detected in males. These results strongly suggest that an abundant supply of Ac protein is required during early embryogenesis, perhaps at the time of cellularization of the blastoderm-nuclei. INTRODUCTNThe genomes of lower eukaryotes such as DrosoUhila and yeast contain sequences related to vertebrate onoogenes (1,2,3,4). Thus it is possible to study cellular oncogene structure and function in different developmental and genetic contexts. V-= gene sequences, representing one of the most extensively studied oncogene families, are among those sequences that are conserved in DlosobIA. Recombinant DNA clones representing three 1rwnb±1a genomic sequences complementary to the v-= gene have been reported (5,6). Nucleotide sequence analysis has confirmed that the v-=-complementarity of two of these genomic sequences is due to significant structural homology, (7) while the third has remained uncharacterized. The studies described in this report were initiated to fill two voids in the knowledge of Droso2LU± =-related sequences; the lack of nucleotide sequence data from the third =-related genomic segment and the lack of detailed knowledge of the expression of the three =-related-genomic C) I RL Press Umited, Oxford, England. Nucleic Acids ResearchVolume 13 Number 6 1985 2 153

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Section: Screening the Bacterionhage Lambda Drosonhila Geoe Librarmentioning

confidence: 60%

Maternal inheritance of transcripts from threeDrosophila src-related genes

Wadsworth¹,

Madhavan

Bilodeau-Wentworth³

1985

Nucl Acids Res

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“…They represent from 0.05 to 0.66% of Thus, neural tissue-specific sequences selected because the polyadenylated mRNA mass of the head (Table 2), DEVELOPMENTAL BIOLOGY VOLUME 94,1982 15AB X l 28A 44CD 47E 28C 32AB 34F 43AB 1 46E I48F 518 57c 2L I I I 2R 66D 72BC 73DEF 82F 92CD 99C 1OOB 3L I I I I I II and thus belong to the moderately abundant mRNA class identified by Levy and McCarthy (1975). Assuming the average Drosophila cell contains 6.6 pg of RNA (Lamb and Laird, 1976) of which 90% is cytoplasmic and 68% of that is polysomal (Lovett and Goldstein, 1977), it is possible to estimate the number of copies of these species per average cell of the head. Since 0.5% of polysomal RNA of adult represents polyadenylated mRNA, the most abundantly expressed head-specific clone, 507, occurs at an average level of 140 copies per cell in the head, but only 0.8 copies per cell in the body.…”

Section: Discussionmentioning

confidence: 99%

The selection, expression, and organization of a set of head-specific genes in Drosophila

Lévy

Ganguly

et al. 1982

Developmental Biology

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Comparative screening of a library of cloned Drosophila DNA with polyadenylated mRNA from Drosophila adult head and adult body identified 20 cloned sequences expressed more abundantly in the tissues of the head than in other tissues. Quantitati.on using a "dot blot" hybridization assay demonstrated that the DNA sequences are expressed on adult head polysomes from 3 to 177 times more abundantly than on body polysomes, and their transcripts represent from 0.05 to 0.6656 of the polyadenylated mRNA mass of the head. The steady-state nuclear RNA concentrations of four species were (determined to be from 13 to 48 times greater in head nuclei than in body nuclei, an indication that their expression is controlled at least in part at the transcriptional level. The chromosomal locations of all 20 headspecific clones were identified by in situ mapping, and no distinct clustering was observed. However, four of the clones were shown by Southern and Northern blot analysis to contain multiple RNA coding sequences. The genes in these tightly clustered ssets were observed to be expressed simultaneously in some cases and differently in others.

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“…A strain of Drosophila melanogaster Oregon R was maintained in large cage populations as described in Lovett and Goldstein (1977). They were fed baker's yeast on agar plates, from which timed embryos were collected as needed.…”

Section: Maintenance Of Stocksmentioning

confidence: 99%