The gene structure of the Drosophila melanogaster proto-oncogene, kayak, and its nested gene, fos-intronic gene

Hudson, S; Goldstein, Elliott S.

doi:10.1016/j.gene.2008.05.001

Cited by 6 publications

(7 citation statements)

References 20 publications

(44 reference statements)

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“…However, we consider it unlikely that the gene responsible for the sro mutation is kay/D-fos for the following reasons. First, all EMSinduced sro mutants are complemented with the validated kay mutant, which is also consistent with a recent report (Hudson and Goldstein, 2008). Second, both of the independent EMS-induced sro alleles have nonsense mutations that disrupt the catalytic centers of the SDR proteins.…”

Section: Discussionsupporting

confidence: 89%

“…4D), suggesting that the gene responsible for the EMS-induced sro mutants must be different from the kay gene. This result was consistent with another recent report (Hudson and Goldstein, 2008). Second, qRT-PCR analysis revealed that the sro P54 homozygous mutant embryos exhibited a large reduction in the CG12068 transcript level compared with the control embryos (Fig.…”

Section: Positional Cloning Of Nm-g In B Morisupporting

confidence: 93%

“…4D). Because another previous study argues that the sro 1 mutant has a point mutation in the first exon of kay (Hudson and Goldstein, 2008), we also re-examined their finding. We found three synonymous nucleotide substitutions, but not any nonsynonymous changes, in the ORF region in the first exon of kay (see Fig.…”

Section: Discussionmentioning

confidence: 97%

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Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the ‘Black Box’ of the ecdysteroid biosynthesis pathway

et al. 2010

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SUMMARYIn insects, the precise timing of molting and metamorphosis is strictly guided by a principal steroid hormone, ecdysone. Among the multiple conversion steps for synthesizing ecdysone from dietary cholesterol, the conversion of 7-dehydrocholesterol to 5-ketodiol, the so-called 'Black Box', is thought to be the important rate-limiting step. Although a number of genes essential for ecdysone synthesis have recently been revealed, much less is known about the genes that are crucial for functioning in the Black Box. Here we report on a novel ecdysteroidgenic gene, non-molting glossy (nm-g)/shroud (sro), which encodes a short-chain dehydrogenase/reductase. This gene was first isolated by positional cloning of the nm-g mutant of the silkworm Bombyx mori, which exhibits a low ecdysteroid titer and consequently causes a larval arrest phenotype. In the fruit fly, Drosophila melanogaster, the closest gene to nm-g is encoded by the sro locus, one of the Halloween mutant members that are characterized by embryonic ecdysone deficiency. The lethality of the sro mutant is rescued by the overexpression of either sro or nm-g genes, indicating that these two genes are orthologous. Both the nm-g and the sro genes are predominantly expressed in tissues producing ecdysone, such as the prothoracic glands and the ovaries. Furthermore, the phenotypes caused by the loss of function of these genes are restored by the application of ecdysteroids and their precursor 5-ketodiol, but not by cholesterol or 7-dehydrocholesterol. Altogether, we conclude that the Nm-g/Sro family protein is an essential enzyme for ecdysteroidogenesis working in the Black Box.

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Section: Discussionsupporting

confidence: 89%

Section: Positional Cloning Of Nm-g In B Morisupporting

confidence: 93%

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Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the ‘Black Box’ of the ecdysteroid biosynthesis pathway

et al. 2010

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“…This gene encodes the D. melanogaster Fos transcription factor (D-Fos) that has four isoforms (Giesen et al, 2003;Hudson and Goldstein, 2008). Mutations in the exon that codes for the N-terminus of two isoforms cause a phenotype that resembles those provoked by ecdysone deficiency although ecdysone levels are normal in the mutant animals (Chavez et al, 2000).…”

Section: Ecdysone Drives Multiple Pathways Of Cuticle Differentiationmentioning

confidence: 99%

Recent advances in understanding mechanisms of insect cuticle differentiation

Moussian

2010

Insect Biochemistry and Molecular Biology

418

318

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“…In humans, c-fos encodes part of the AP-1 transcription factor and is known to be misregulated in a number of tumors (Perkins et al 1988). Utilizing genome annotations for D. melanogaster and confirmation with a variety of mRNA-based techniques, we generated a new model for the structure of kay (Hudson 2006). That study showed that kay is a substantial gene (27.5 kb) with three distinct promoters.…”

mentioning

confidence: 99%

Phylogenetic and Genomewide Analyses Suggest a Functional Relationship Betweenkayak, the Drosophila Fos Homolog, andfig, a Predicted Protein Phosphatase 2C Nested Within akayakIntron

et al. 2007

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A gene located within the intron of a larger gene is an uncommon arrangement in any species. Few of these nested gene arrangements have been explored from an evolutionary perspective. Here we report a phylogenetic analysis of kayak (kay) and fos intron gene ( fig), a divergently transcribed gene located in a kay intron, utilizing 12 Drosophila species. The evolutionary relationship between these genes is of interest because kay is the homolog of the proto-oncogene c-fos whose function is modulated by serine/threonine phosphorylation and fig is a predicted PP2C phosphatase specific for serine/threonine residues. We found that, despite an extraordinary level of diversification in the intron-exon structure of kay (11 inversions and six independent exon losses), the nested arrangement of kay and fig is conserved

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The gene structure of the Drosophila melanogaster proto-oncogene, kayak, and its nested gene, fos-intronic gene

Cited by 6 publications

References 20 publications

Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the ‘Black Box’ of the ecdysteroid biosynthesis pathway

Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the ‘Black Box’ of the ecdysteroid biosynthesis pathway

Recent advances in understanding mechanisms of insect cuticle differentiation

Phylogenetic and Genomewide Analyses Suggest a Functional Relationship Betweenkayak, the Drosophila Fos Homolog, andfig, a Predicted Protein Phosphatase 2C Nested Within akayakIntron

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