The cytocidal effect of high energy shock waves on human prostatic tumour cell lines

Dongen, Jan Willem Van; Steenbrugge, G.J. van; Romijn, J. A.; Schröder, Fritz H.

doi:10.1016/0277-5379(89)90411-2

Cited by 27 publications

(12 citation statements)

References 12 publications

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“…The significant extracellular efflux of these enzymes in the present study reflects damage to the cell membrane. Dam age to the cell membrane of the cells treated with HESW has been confirmed ultrastructurally in some reports [7,15], The mechanism by which HESW induces cell mem brane damage has not been clarified yet, but may be attributable to microcavitation [8].…”

Section: Discussionmentioning

confidence: 94%

In vitro Effects of High Energy Shock Wave Alone and Combined with Anticancer Drugs on Human Bladder Cancer Cells

Rahman

1994

Urol Int

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We investigated the in vitro effects of high energy shock wave alone or combined with anticancer drug treatment on an established human bladder cancer cell line, KK-47 cells. Exposure of the cell suspensions to 500-2,500 shock waves, generated with the EDAP lithotriptor LTO1 resulted in reduced cell viability with an increasing number of shock doses. DNA flow cytometric analysis revealed a marginal effect on the G2+M and S phases of the cell cycle. When shock wave-treated cells were exposed to mitomycin C, cisplatin, methotrexate or adriamycin, an additive cytotoxic effect was obtained at all shock doses. Adriamycin showed the highest additive effect of the drugs tested. Significant increases in glutamic oxaloacetic transaminase, alkaline phosphatase, creatine phosphokinase and lactate dehydrogenase concentrations in the nutrient medium were observed at over 400 shock doses, reflecting damage to the cell membrane. These data suggest that high energy shock wave may be applicable in combination with some anticancer drugs in the treatment of transitional cell carcinoma.

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Section: Discussionmentioning

confidence: 94%

In vitro Effects of High Energy Shock Wave Alone and Combined with Anticancer Drugs on Human Bladder Cancer Cells

Rahman

1994

Urol Int

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“…Renal tissue injury induced by shock waves is also de scribed [10][11][12], Russo et al [3] have demonstrated a dose-dependent cytotoxic effect of HESW. Since these initial experiments, a number of studies using different tumor types have been published [4,[13][14][15], The ob served antitumor effects were induced by necrosis and, as reported by Hoshi et al [2], medicated primarily by vas cular injury. However, the detailed mechanisms of the destruction effects of HESW on the living cells is un known.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Human Renal Cell Carcinoma with High-Energy Shock Waves–A New in vivo/in vitro Model

Roessler¹,

Rothgangel²,

Hofstaedter³

1995

Urol Int

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The effects of high-energy shock waves (HESW) on the human renal cell carcinoma were examined. The kidneys were available from 32 patients treated by radical nephrectomy due to renal cell carcinoma. Immediately after nephrectomy the kidneys were perfused with cold HTK solution and stored for a maximum of 4h in hypothermia at 8°C. The tumors were treated with 4,000 shocks (65 mPa = 0.6 mJ/mm²) in an electromagnetic lithotriptor (Siemens Co., Erlangen, Germany). Microscopic and immunohistological examinations of the tumors were performed after treatment, and cell proliferation rates of treated and untreated specimens were analyzed by cell cultures in 10 cases. HESW induce severe microscopic damage in the tumor tissue as complete rupture of the vessel walls and destruction of the tubular-formed tumor masses in the focal area. Immunohistochemistry shows intact immune reactive endothelial cells by factor 8-associated antibodies until the border to histological damage. Around this region a zone of negative antibody reaction against collagen type 4 is found. In cell cultures the proliferation rates of treated specimens were significantly lower compared to untreated. The human renal cell carcinoma seems to be susceptible for treatment with shock waves. HESW induce direct damage of tumor cells and vascular damage in the tumor which may be the primary cause of tumor necrosis.

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“…This hypothesis is supported on the one hand by studies investigating the effects of direct sound field changes by using different driving voltages of the shockwave device. Results by Dongen et al who treated a suspension of human prostate carcinoma cells showed differing cell survivals associated with the application of different shockwave fields (driving voltages 14.5 vs. 18.1 kV) [ 33 ]. And Maier et al discovered a change in tensile strength of gastrocnemius tendons of turkeys following shockwave treatment with 25 kV (energy density of 1.2 mJ/mm 2 ) in contrast to no measurable reaction at 15 kV (0.6 mJ/mm 2 ) [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…The influence of the setup on the biological reaction has been demonstrated for example by Cleveland et al in whose experiments the cell injury was significantly altered by a slight change of the shockwave field caused by different curvatures of the cell tubes [ 35 ]. Furthermore, experiments by Dongen et al, Steinbach et al, and Laudone et al revealed a dependence of the cell death on air trapped inside cell tubes [ 33 , 37 , 38 ]. These examples show clearly that study outcomes can be strongly influenced by the experimental setup.…”

Section: Introductionmentioning

confidence: 99%

In-vitro cell treatment with focused shockwaves—influence of the experimental setup on the sound field and biological reaction

et al. 2016

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BackgroundTo improve understanding of shockwave therapy mechanisms, in vitro experiments are conducted and the correlation between cell reaction and shockwave parameters like the maximum pressure or energy density is studied. If the shockwave is not measured in the experimental setup used, it is usually assumed that the device’s shockwave parameters (=manufacturer’s free field measurements) are valid. But this applies only for in vitro setups which do not modify the shockwave, e.g., by reflection or refraction. We hypothesize that most setups used for in vitro shockwave experiments described in the literature influence the sound field significantly so that correlations between the physical parameters and the biological reaction are not valid.MethodsTo reveal the components of common shockwave in vitro setups which mainly influence the sound field, 32 publications with 37 setups used for focused shockwave experiments were reviewed and evaluated regarding cavitation, cell container material, focal sound field size relative to cell model size, and distance between treated cells and air. For further evaluation of the severity of those influences, experiments and calculations were conducted.ResultsIn 37 setups, 17 different combinations of coupling, cell container, and cell model are described. The setup used mainly is a transducer coupled via water to a tube filled with a cell suspension. As changes of the shockwaves’ maximum pressure of 11 % can already induce changes of the biological reaction, the sound field and biological reactions are mainly disturbed by use of standard cell containers, use of coupling gel, air within the 5 MPa focal zone, and cell model sizes which are bigger than half the −6 dB focal dimensions.ConclusionsUntil now, correct and sufficient information about the shockwave influencing cells in vitro is only provided in 1 of 32 publications. Based on these findings, guidelines for improved in vitro setups are proposed which help minimize the influence of the setup on the sound field.Electronic supplementary materialThe online version of this article (doi:10.1186/s40349-016-0053-z) contains supplementary material, which is available to authorized users.

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The cytocidal effect of high energy shock waves on human prostatic tumour cell lines

Cited by 27 publications

References 12 publications

In vitro Effects of High Energy Shock Wave Alone and Combined with Anticancer Drugs on Human Bladder Cancer Cells

In vitro Effects of High Energy Shock Wave Alone and Combined with Anticancer Drugs on Human Bladder Cancer Cells

Treatment of Human Renal Cell Carcinoma with High-Energy Shock Waves–A New in vivo/in vitro Model

In-vitro cell treatment with focused shockwaves—influence of the experimental setup on the sound field and biological reaction

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