The CuZn superoxide dismutase from Sclerotinia sclerotiorum is involved with oxidative stress tolerance, virulence, and oxalate production

Veluchamy, Selvakumar; Williams, Brett; Kim, Kyoung-Su; Dickman, Martin B.

doi:10.1016/j.pmpp.2011.12.005

Cited by 50 publications

(26 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were obtained with another OA-deficient strain of S. sclerotiorum , Δsod1 (Fig. S3, [22]). The lesion size and rate of spread varied and was more pronounced in the atg8 mutant lines.…”

Section: Resultssupporting

confidence: 85%

Cell Death Control: The Interplay of Apoptosis and Autophagy in the Pathogenicity of Sclerotinia sclerotiorum

2013

Self Cite

View full text Add to dashboard Cite

Programmed cell death is characterized by a cascade of tightly controlled events that culminate in the orchestrated death of the cell. In multicellular organisms autophagy and apoptosis are recognized as two principal means by which these genetically determined cell deaths occur. During plant-microbe interactions cell death programs can mediate both resistant and susceptible events. Via oxalic acid (OA), the necrotrophic phytopathogen Sclerotinia sclerotiorum hijacks host pathways and induces cell death in host plant tissue resulting in hallmark apoptotic features in a time and dose dependent manner. OA-deficient mutants are non-pathogenic and trigger a restricted cell death phenotype in the host that unexpectedly exhibits markers associated with the plant hypersensitive response including callose deposition and a pronounced oxidative burst, suggesting the plant can recognize and in this case respond, defensively. The details of this plant directed restrictive cell death associated with OA deficient mutants is the focus of this work. Using a combination of electron and fluorescence microscopy, chemical effectors and reverse genetics, we show that this restricted cell death is autophagic. Inhibition of autophagy rescued the non-pathogenic mutant phenotype. These findings indicate that autophagy is a defense response in this necrotrophic fungus/plant interaction and suggest a novel function associated with OA; namely, the suppression of autophagy. These data suggest that not all cell deaths are equivalent, and though programmed cell death occurs in both situations, the outcome is predicated on who is in control of the cell death machinery. Based on our data, we suggest that it is not cell death per se that dictates the outcome of certain plant-microbe interactions, but the manner by which cell death occurs that is crucial.

show abstract

Section: Resultssupporting

confidence: 85%

Cell Death Control: The Interplay of Apoptosis and Autophagy in the Pathogenicity of Sclerotinia sclerotiorum

2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Still, there is difficulty in deriving a comparison for the role of antioxidation genes and enzymes across fungal lifestyles due to a disagreement between the function of the components during these interactions. For example, in agreement with the role of SOD for B. cinerea infection, S. sclerotiorum also requires SOD for pathogenicity due to its role in oxalate production, a secreted metabolite required for pathogenicity (Veluchamy et al, 2012). However, in contrast to the role of CatB in the necrotrophs B. cinerea and C. heterostophus, catalases were required for pathogenicity by S. sclerotiorum but not for scavenging extracellular host-derived ROS, which is similar to the role of CatB described for M. oryzae (Robbertse et al, 2003;Schouten et al, 2002;Yarden et al, 2014).…”

Section: Necrotrophsmentioning

confidence: 76%

Reactive oxygen species metabolism and plant-fungal interactions

Segal

Wilson

2018

Fungal Genetics and Biology

148

View full text Add to dashboard Cite

Fungal interactions with plants can involve specific morphogenetic developments to access host cells, the suppression of plant defenses, and the establishment of a feeding lifestyle that nourishes the colonizer oftenbut not always-at the expense of the host. Reactive oxygen species (ROS) metabolism is central to the infection process, and the stage-specific production and/or neutralization of ROS is critical to the success of the colonization process. ROS metabolism during infection is dynamicsometimes seemingly contradictory-and involves endogenous and exogenous sources. Yet, intriguingly, molecular decision-making involved in the spatio-temporal control of ROS metabolism is largely unknown. When also considering that ROS demands are similar between pathogenic and beneficial fungal-plant interactions despite the different outcomes, the intention of our review is to synthesize what is known about ROS metabolism and highlight knowledge gaps that could be hindering the discovery of novel means to mediate beneficial plant-microbe interactions at the expense of harmful plant-microbe interactions.

show abstract

“…2), suggesting that these genes may have an important role in the virulence of the fungus, preventing the ROSdependent defence of the fruit. The involvement in pathogenicity of superoxide dismutase (SOD) and NADPH:ubiquinone oxidoreductase has been demonstrated in other necrotrophic fungi (Rolke et al, 2004;Seong et al, 2005;Veluchamy et al, 2012;Xu and Chen, 2012), whereas other fungal ROS-related genes, such as catalase and glutathione-S-transferase, seem to be dispensable for full virulence (Prins et al, 2000;Schouten et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of Penicillium digitatum genes putatively involved in virulence towards citrus fruit

López‐Pérez

Ballester

González-Candelas

2014

Molecular Plant Pathology

View full text Add to dashboard Cite

The fungus Penicillium digitatum, the causal agent of green mould rot, is the most destructive post-harvest pathogen of citrus fruit in Mediterranean regions. In order to identify P. digitatum genes up-regulated during the infection of oranges that may constitute putative virulence factors, we followed a polymerase chain reaction (PCR)-based suppression subtractive hybridization and cDNA macroarray hybridization approach. The origin of expressed sequence tags (ESTs) was determined by comparison against the available genome sequences of both organisms. Genes coding for fungal proteases and plant cell wall-degrading enzymes represent the largest categories in the subtracted cDNA library. Northern blot analysis of a selection of P. digitatum genes, including those coding for proteases, cell wall-related enzymes, redox homoeostasis and detoxification processes, confirmed their up-regulation at varying time points during the infection process. Agrobacterium tumefaciens-mediated transformation was used to generate knockout mutants for two genes encoding a pectin lyase (Pnl1) and a naphthalene dioxygenase (Ndo1). Two independent P. digitatum Δndo1 mutants were as virulent as the wild-type. However, the two Δpnl1 mutants analysed were less virulent than the parental strain or an ectopic transformant. Together, these results provide a significant advance in our understanding of the putative determinants of the virulence mechanisms of P. digitatum.

show abstract

The CuZn superoxide dismutase from Sclerotinia sclerotiorum is involved with oxidative stress tolerance, virulence, and oxalate production

Cited by 50 publications

References 48 publications

Cell Death Control: The Interplay of Apoptosis and Autophagy in the Pathogenicity of Sclerotinia sclerotiorum

Cell Death Control: The Interplay of Apoptosis and Autophagy in the Pathogenicity of Sclerotinia sclerotiorum

Reactive oxygen species metabolism and plant-fungal interactions

Identification and functional analysis of Penicillium digitatum genes putatively involved in virulence towards citrus fruit

Contact Info

Product

Resources

About