The cup-plate method in microbiological assay, with special reference to riboflavine and aneurine

Application of the disk method to cultured plant cells. II. Exhibition zones

“…The diameter of the zone could therefore be used to estimate unknown concentrations of metabolites. A linear relationship between log concentration and zone diameter was also found in microbiological assays of vitamins [ 1 ].…”

Pediococcus acidilactici NCIB 6990, a New Test Organism for Microbiological Assaying of Pantothenic Acid

“…An earlier method, which appears in Pharmacopoea Nordica (1 966), consists of cultivating a LactobaciZZus plantarum strain in liquid medium, to which the vitamin is added in increasing concentrations, and determining growth by turbidity measurements. Bacharach & Cuthbertson (1948), Morris & Jones (1953) have used the diffusion method for the microbiological assay of vitamins and they claimed that although diffusion methods are somewhat lacking in sensitivity, the speed with which they may be carried out compensates for this. For this reason we decided to determine whether regression curves display a satisfactory straight line relationship between a geometrical dilution series of the vitamin and the corresponding growth zone diameter for each dilution stage using the diffusion method and P .…”

Plate Assay of Thiamine

“…Lactobacillus fermenti strain 36 is the most frequently used microorganism for the assay of thiamine both in the tube method, (Sarett and Cheldelin, 1944;Fitzgerald and Hughes, 1949), and in the plate method, (Bacharach and Cuthbertson, 1948;Jones and Morris, 1949). Other microorganisms have more recently been suggested for the tube assay; Hoff- Jorgensen and Hansen (1955) used the yeast Kloeckera brevis, and Deibel et al (1958) used Lactobacillus viridescens.…”