The culture filtrates from Bacillus subtilis natto lowers blood pressure via renin-angiotensin system in spontaneously hypertensive rats fed with a high-cholesterol diet

Abstract

Antihypertensive effect of the culture filtrates from Bacillus subtilis natto (CFB) associated with renin-angiotensin system (RAS) as well as fibrinolytic and anti-thrombotic activity was examined. By using the spontaneously hypertensive rats fed with a high-cholesterol diet (0.5% cholesterol and 0.5% cholic acid), biomarkers related to the RAS were measured after 11 week-administration of CFB in the diet at 1 or 2% levels. Systolic blood pressure was significantly attenuated by CFB at both levels. Plasma renin activity was significantly suppressed by CFB, and protein expression of renin in kidney also showed tendency of decrease by administration of CFB, although not statistically significant. Renal angiotensin II concentrations were decreased, whereas plasma angiotensin II concentrations showed no differences among groups. Whole blood clotting and euglobulin clot lysis time showed insignificant changes, but downward tendencies by CFB supplementation. Although further study is necessary to clearly elucidate the related mechanism, taken together, these findings support the hypothesis that CFB could be potentially used as dietary supplement to suppress hypertension.

Enhancing menaquinone-7 production in recombinant Bacillus amyloliquefaciens by metabolic pathway engineering

“…MK-7 is part of a family of MK-ns with a side chain of seven isoprene units, and has unique physiological functions, such as promoting the formation of bone and reducing blood pressure. 1,5,6 In the present study, we revealed the difference in the head structure biosynthesis enzymes involved in the MK-7 biosynthetic pathway starting from isochorismate based on the genetic level between strains Y-2 and W-21 that are distinct in MK-7 production, and demonstrate the importance of six enzymes with amino acid variations for increasing MK-7 production. Moreover, the present work points out that the same enzyme has different effects on MK-7 production for different cultivating methods.…”

“…5 In addition, it can also lower blood pressure in rats. 6 Therefore, MK-7 is receiving increasing attention in the domain of nutritional supplements for humans.…”

Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance

“…MK-7 can activate the osteocalcin in bone marrow to promote the formation of bone, and can improve the bone mineral density (BMD) in the aged so as to reduce the risk of haunch bone fracture (Howard and Payne, 2006). In addition, it can lower blood pressure in rats (Kim et al, 2011). Food products, such as sauerkraut, cheese, or fermented soybean, may be predominant sources of dietary MK-7 in many parts of the world (Berenjian et al, 2011).…”

“…According to the literature, many microorganisms can be used to biosynthesize MK-7, such as Bacillus subtilis and Escherichia coli (Howard and Payne, 2006;Berenjian et al, 2011). Most MK-7 is produced by Bacillus sp., which is isolated from natto (Berenjian et al, 2011;Kim et al, 2011). However, researchers have focused on genetic mutation to improve the productivity of the microorganism because of the low MK-7 content in fermentation processes.…”

The Evaluation of Antithrombotic and Fibrinolytic Activities of Nattokinase from Bacillus subtilis Natto

“…2). 기존에 인체 및 설치류를 대상으로 한 nattokinase 혈행개 선 연구에서, nattokinase 경구섭취시 혈압이 저해됨을 확인 되었다[5,7,[18][19][20]. 또한 설치류를 대상으로 한 in vivo, in vitro 동물모델적용의 결과 중국의 전통 대두발효식품인 Douchi에서 분리된 Bacillus subtilis가 생산하는 섬유소 분해 효소에 의해 농도 의존적으로 bleeding time이 최대 1.3배 지연 되었고 응고 시간은 1.5배 지연되는 결과를 나타내었다[20].…”