The Evaluation of Antithrombotic and Fibrinolytic Activities of Nattokinase from Bacillus subtilis Natto

Abstract

1) We previously reported that Ultra nattokinase Ⓡ showed high fibrinolytic activity and revealed antithrombotic effect in rat blood plasma based on its ability to suppress collagen-induced platelet aggregation. This research was carried out to verify the clot lysing activity and blood flow enhancing effects of Ultra nattokinase Ⓡ via monitoring and comparing the antithrombotic effects in rat artery between oral administration of Ultra nattokinase Ⓡ and maltodextrin. SD rats were fed with 1.11 mg/kg of Ultra nattokinase Ⓡ for 4 weeks. The effect on arterial thrombosis was then evaluated using an antithrombotic model after induction by FeCl3. Detected fibrinolytic activity was proportional to the content of Ultra nattokinase Ⓡ and statistical extents of the antithrombotic activity was enhanced strongly twice rather than control group. The PT and the aPTT, however, showed only a small difference between two groups. The results suggest that Ultra nattokinase Ⓡ can effectively treat thromboembolism and enhance blood flow, and that Ultra nattokinase Ⓡ can also prevent venous occlusion by aiding clot lysis. 연세대학교 의과대학 심혈관제품 유효성평가센터 Cardiovascular product evaluation center Yeonsei university collage of medicine, 50 Yonsei-ro,