The crystallization of lysozyme in the system of ionic liquid [BMIm][BF<sub>4</sub>]‐water

Li, Xinxin; Xu, Xiaodong; Dan, Y. Y.; Feng, Jing; Wang, Chuanzhen; Zhang, Milin

doi:10.1002/crat.200800040

Cited by 28 publications

(20 citation statements)

References 40 publications

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“…They assumed the BF 4 − , the anion of ILs, adhered to the surface of the lysozyme crystal, which resulted in the obstruction of the crystal growth [20]. We presumed that the variation of crystal morphology of sucrose crystals precipitated under different ionic liquid conditions has similar aspects with the result reported by Li et al It could be concluded as such because the anion was the variable, and the cation was the control in this study.…”

Section: Intramolecular Intermolecular Periodic Bonds Chains (Pbcs)supporting

confidence: 78%

“…They assumed the BF4 − , the anion of ILs, adhered to the surface of the lysozyme crystal, which resulted in the obstruction of the crystal growth [20]. We presumed that the variation of crystal morphology of sucrose crystals precipitated under different ionic liquid conditions has similar aspects with the result reported by Li As observed in Figures 3 and 4, crystals have similar structures, but different crystal morphologies; this is due to differences in the adhesion of additional solvent, impurities, additives, etc., to the crystal surface, or due to the differences in the supersaturation level [37].…”

Section: Intramolecular Intermolecular Periodic Bonds Chains (Pbcs)mentioning

confidence: 99%

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Effect of Ionic Liquids on the Separation of Sucrose Crystals from a Natural Product Using Crystallization Techniques

Kiyonga

Yoon

et al. 2017

Crystals

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Abstract:The present work aims to investigate the applicability of ionic liquids (ILs) for natural ingredient crystallization. First, the medicinal plant, namely Angelica gigas Nakai, was extracted using methanol (MeOH) as a solvent. Afterwards, ILs 1-butyl-3-methylimidazolium tetrafluoroborate (BMImBF 4 ), 1-butyl-3-methylimidazolium hexafluorophosphate (BMImPF 6 ), 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (BMImTFSI), 1-allyl-3-ethylim idazolium tetrafluoroborate (AEImBF 4 ), and 1,3-diallyl imidazolium tetrafluoroborate (AAImBF 4 ), in three ratios of 1:1, 1:2, and 1:3 (extraction solution/ILs (v/v)) were used as an anti-solvent to induce crystallization. Crystals were obtained within 8 h and were then identified to be pure crystals of sucrose through nuclear magnetic resonance ( 1 H-NMR) analysis. Moreover, the single-crystal X-ray diffraction (SXD) analysis revealed all recovered crystals have an identical crystal structure and the morphology was monitored using a video microscope. With the application of BMImBF 4 and BMImPF 6 , transformation of sucrose crystal morphology from an elongated hexagon shape to an elongated rectangular shape was observed with respect to the respective concentration increase. Here, all crystals precipitated from BMImBF 4 and BMImPF 6 were found to possess identical PXRD patterns. However, when BMImTFSI was employed, small rectangular crystals attached to the larger rectangular-shaped crystals due to secondary nucleation and shapeless amorphous forms were observed according to the alteration in the solution to ILs ratio. Accordingly, the ability of ILs as a relevant anti-solvent for the selective crystallization of a single compound from a natural product was assessed through the study. Furthermore, the applicability of ILs as crystal engineering solvents are expected to modify both the solid state and the crystal morphology of natural compounds, which can influence drug manufacturability, dissolution rate, and bioavailability.

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Section: Intramolecular Intermolecular Periodic Bonds Chains (Pbcs)supporting

confidence: 78%

“…They assumed the BF4 − , the anion of ILs, adhered to the surface of the lysozyme crystal, which resulted in the obstruction of the crystal growth [20]. We presumed that the variation of crystal morphology of sucrose crystals precipitated under different ionic liquid conditions has similar aspects with the result reported by Li As observed in Figures 3 and 4, crystals have similar structures, but different crystal morphologies; this is due to differences in the adhesion of additional solvent, impurities, additives, etc., to the crystal surface, or due to the differences in the supersaturation level [37].…”

Section: Intramolecular Intermolecular Periodic Bonds Chains (Pbcs)mentioning

confidence: 99%

Effect of Ionic Liquids on the Separation of Sucrose Crystals from a Natural Product Using Crystallization Techniques

Kiyonga

Yoon

et al. 2017

Crystals

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“…However, the impact of ionic liquids on the crystallization process is more pronounced than for precipitation [92,94]. Several authors report on less crystal polymorphism and improved tolerance to concomitant impurities during crystallization [94][95][96][97][98][99].…”

Section: Protein Crystallizationmentioning

confidence: 99%

Proteins in Ionic Liquids: Current Status of Experiments and Simulations

Schröder¹

2017

Topics in Current Chemistry Collections

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In the last two decades, while searching for interesting applications of ionic liquids as potent solvents, their solvation properties and their general impact on biomolecules, and in particular on proteins, gained interest. It turned out that ionic liquids are excellent solvents for protein refolding and crystallization. Biomolecules showed increased solubilities and stabilities, both operational and thermal, in ionic liquids, which also seem to prevent self-aggregation during solubilization. Biomolecules can be immobilized, e.g. in highly viscous ionic liquids, for particular biochemical processes and can be designed to some extent by the proper choice of the ionic liquid cations and anions, which can be characterized by the Hofmeister series.

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“…In recent years, there has been an increasing attention to the application of ionic liquids in different areas of the protein science 1 -from protein stability, [2][3][4][5] activity, [6][7][8] and extraction [9][10][11][12] to protein crystallization. [13][14][15][16][17][18][19][20] One of the reasons for this relevance is the tunable nature of ILs that allows tailoring ILs with specific chemical and physical properties. 21 In regard to protein crystallization, studies have mainly focused on model proteins in order to understand the origin of the ILs influence on biomacromolecular crystals formation.…”

Section: Introductionmentioning

confidence: 99%

Hofmeister effects of ionic liquids in protein crystallization: Direct and water-mediated interactions

et al. 2012

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We have performed experiments on the crystallization of two low molecular weight, positively charged proteins, lysozyme and ribonuclease A, using ionic liquids as either crystallization additives or, in particular cases, as precipitating agents. The ionic liquids (ILs) have been ordered according to their salting-in/out ability and the relative position of these ionic liquids in this ranking has been rationalized by considering their hydration properties (positive-negative, hydrophobichydrophilic). The ability to screen the effective charge of cationic proteins and aid protein nucleation (salting-out) has been shown to be superior for large polarizable anions with low charge density, negatively hydrated-Cl 2 , Br 2 , [SCN] 2 , methane-[C 1 SO 3 ] 2 and ethanesulfonates [C 2 SO 3 ] 2 , than for anions with a relatively stable hydration shell, positively hydrated-lactate [Lac] 2 , butylsulfonate [C 4 SO 3 ] 2 and acetate [Ac] 2. Upon increasing the background salt concentration, where electrostatic interactions are already effectively screened, the ability of the IL ions to stabilize proteins in solution (salting-in) has been shown to increase as the ions are likely to migrate to the non-polar protein surface and lower protein-water interfacial tension. This tendency is enhanced as the focus moves from those ions with positively hydrated hydrophilic compartments (e.g. [Ac] 2) to those with negatively hydrated groups (e.g. [C 1 SO 3 ] 2) and the prevailing hydrophobic hydration (e.g. [C 4 SO 3 ] 2). The observed inversion in the relative effect of ILs on protein crystallization with increasing ionic strength of the aqueous media has been interpreted as the differing effects of ion adsorption: charge screening and interfacial tension modification. Moreover, this work can further help in our understanding of the influence of ionic liquids on conformational changes of biomacromolecules in solution. Identification of the specific incorporation sites for choline and acetate ions, localized in two lysozyme crystals grown in pure IL solutions without any buffer or inorganic precipitant, can give us some insight into the role of the ionic liquid ions in protein structure development.

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The crystallization of lysozyme in the system of ionic liquid [BMIm][BF₄]‐water

Cited by 28 publications

References 40 publications

Effect of Ionic Liquids on the Separation of Sucrose Crystals from a Natural Product Using Crystallization Techniques

Effect of Ionic Liquids on the Separation of Sucrose Crystals from a Natural Product Using Crystallization Techniques

Proteins in Ionic Liquids: Current Status of Experiments and Simulations

Hofmeister effects of ionic liquids in protein crystallization: Direct and water-mediated interactions

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The crystallization of lysozyme in the system of ionic liquid [BMIm][BF4]‐water

Cited by 28 publications

References 40 publications

Effect of Ionic Liquids on the Separation of Sucrose Crystals from a Natural Product Using Crystallization Techniques

Effect of Ionic Liquids on the Separation of Sucrose Crystals from a Natural Product Using Crystallization Techniques

Proteins in Ionic Liquids: Current Status of Experiments and Simulations

Hofmeister effects of ionic liquids in protein crystallization: Direct and water-mediated interactions

Contact Info

Product

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The crystallization of lysozyme in the system of ionic liquid [BMIm][BF₄]‐water