The Cryopreservation of Striped Bass Morone saxatilis Semen

Abstract

Abstract.— Two experiments were designed to improve upon existing methods for cryopreserving striped bass Morone saxatilis, semen. In the first experiment, two extenders, two cryoprotectant concentrations, and two freezing rates were evaluated on the basis of post‐thaw semen motility after 1, 7, and 30 d of storage at −196 C. Semen samples cryopreserved at a freezing rate of −40 C/m resulted in a significantly higher percentage of motile sperm (P < 0.001) and longer duration of spermatozoa motility (P < 0.001) than samples cryopreserved at a freezing rate of ‐30 Chin. Also, the cryoprotectant dimethyl‐sulfoxide yielded a significantly higher percentage of motile sperm (P < 0.001) and longer duration of spermatozoa motility (P < 0.001) when a 5% concentration was used instead of 7.5%. In the second experiment, the two extenders from Experiment I were re‐evaluated and a new extender, which was a modified version of Extender 1, was tested. The samples were cryopreserved at ‐40 C/min with 5% DMSO and thawed in a 25 C water bath. Spermatozoa motility and fertilization ability were evaluated, and semen cryopreserved in Extender 2 yielded the longest duration of spermatozoa motility (P < 0.001). the highest percentage of motile sperm (P < 0.001). and the highest percentage of fertilized eggs (P < 0.002) in comparison to Extenders I and 3.

Fine structure, motility and cryopreservation of spotted sea bass, Lateolabrax maculatus (Moronidae, Teleostei), spermatozoa

“…Sodium chloride at concentration of 150 m m plus 5% DMSO proved to be a suitable diluent for spotted sea bass and striped bass sperm (Brown and Brown, 2000; Jenkin‐Keeran and Woods, 2002; Zilli et al, 2003, 2004, 2005). Billard (1984) and Sansone et al.…”

An Evaluation of Extenders for the Short-Term Storage of Striped Bass Milt

“…It is interesting to note that the best extender for the cryopreservation of striped bass milt was not the best extender for the short-term preservation of striped bass milt. When extenders 1, 2, and 5 were used to cryopreserve striped bass milt, extender 2 yielded significantly higher sperm motility and fertilization percentages than did extenders 1 and 5 (Jenkins-Keeran and Woods 2002).…”

“…Extender 1 has been used successfully in the cryopreservation of milt from rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta (Stein and Bayrle 1978), as well as from Arctic char Salvelinus alpinus (Piironen 1993). Extender 2 is used by many researchers for the short-term, refrigerated storage and cryopreservation of striped bass milt (Brown and Brown 2000;Jenkins-Keeran et al 2001;Jenkins-Keeran and Woods 2002). Extender 3 was developed to mimic the mean osmolality, pH, sodium, and potassium measured from milt samples collected from 15 striped bass near the peak of the spawning season in 1997.…”

“…To ameliorate these problems, researchers have examined procedures such as cryopreservation (Kerby 1983(Kerby , 1984Brown and Brown 2000;Jenkins-Keeran and Woods 2002), the domestication of broodstock (Leffler 1999;Woods et al 1999), and control of the reproductive cycle (Woods and Sullivan 1993;Sullivan et al 1997). Another potential solution is the short-term, refrigerated storage of striped bass milt.…”

Selective breeding for the hybrid striped bass (Morone chrysops, Rafinesque xM. saxatilis, Walbaum) industry: status and perspectives

“…1997; Sullivan, Hiramatsu, Kennedy, Clark, Weber, Matsubara & Hara 2003; Clark, Henderson‐Arzapalo & Sullivan 2005), making it possible to reproduce captive striped bass and white bass broodstocks at commercial scale (Mylonas, Magnus, Klebanov, Gissis & Zohar 1997; Hodson, Clark, Hopper, McGinty, Weber & Sullivan 2000). To ensure that germplasm from both species is protected, distributed and efficiently utilized, cryopreservation research being conducted on Morone species has delivered practical protocols for short‐term storage (Jenkins‐Keeran, Schreuders, Edwards & Woods 2001; Jenkins‐Keeran & Woods 2002a; He & Woods 2003a; He, Jenkins‐Keeran & Woods 2004) and long‐term cryopreservation (Jenkins‐Keeran & Woods 2002b; He & Woods 2003b, 2004a, b) of semen.…”