Fine structure, motility and cryopreservation of spotted sea bass, Lateolabrax maculatus (Moronidae, Teleostei), spermatozoa

Abstract

Both transmission-and scanning electron microscopy were used to investigate the fine structure of spermatozoa of the spotted sea bass (Lateolabrax maculatus). The spermatozoa are of the ect-aquasperm type, with a head lacking acrosome, midpiece, and tail regions. The centriolar complex is situated outside the nuclear fossa and the flagellum is parallel to the base of the nucleus. A midpiece, containing four mitochondria, extends from the posterior end of the nucleus and encircles the root of the flagellum. A pyramidal basal foot projects laterally at right angles from the midregion of the basal body. The effects of osmolality and pH on activation of spotted sea bass sperm motility were examined and compared with those of the moronids Dicentrarchus labrax and Morone saxatilis. A simple method of cryopreservation semen was also demonstrated. This study contributes to existing knowledge of comparative sperm biology and may facilitate breeding of this economically important species.

Effects of Trehalose, Bovine Serum Albumin, and Sucrose, on the Integrity of the Plasma Membrane of Pseudosciaena crocea Semen after Cryopreservation

“…After 5 h, the samples were dehydrated with graded ethanol, followed by four changes of tert-butyl alcohol (TBA). Semen were then criticalpoint dried and gold coated (Wu et al, 2007;Gwo, 2010). The ultrastructure of sperm was observed under a JSM-S3400N scanning electron microscope.…”

Influence of an exogenous androgen, testosterone propionate on milt quality and short-term preservation of milt of Asian seabass Lates Calcarifer

“…Major problems which affect short-term storage of milt are temperature, gaseous exchange, microbial attack and desiccation (Fauvel et al, 1998;Pereira 1999;Fauvel et al, 2012). Reports show that anaerobic storage of sperm at 4°C resulted in low motility and aerobic storage (in oxygen) at the same temperature showed high fertility rate (94%) after 216 h (Saad et al, 1988;Chereguini et al, 1997;Gwo, 2010). The enhanced, sperm motility; motility duration and viability recorded in the present study can be correlated to the presence of oxygen in the medium as reported above.…”

Improvement of chilled seabass sperm conservation using a cell culture medium

“…Then specific cryoprotectants are added. For example, the dilution of Lateolabrax maculeatus sperm in a simple NaCl solution with either 5 or 10% dimethyl sulfoxyde (DMSO) was sufficient to protect it at freezing (Gwo, ).…”