The Crude Extract from<i>Puerariae</i>Flower Exerts Antiobesity and Antifatty Liver Effects in High-Fat Diet-Induced Obese Mice

Kamiya, Tomoyasu; Sameshima-Kamiya, Mayu; Nagamine, Rika; Tsubata, Masahito; Ikeguchi, Motoya; Takagaki, Kinya; Shimada, Tsutomu; Aburada, Masaki

doi:10.1155/2012/272710

Cited by 15 publications

(12 citation statements)

References 25 publications

(25 reference statements)

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“…Liu et al reported that PFE improves cognitive impairment in diabetic mice through the normalisation of acetylcholinesterase (AChE) activity and metabolic abnormalities in the brain [28]. Kamiya et al demonstrated that PFE has anti-fatty liver and antiobesity effects in high-fat diet-induced obese mice by suppressing lipogenesis in the liver, stimulating lipolysis in white adipose tissue, and promoting thermogenesis in brown adipose tissue [29]. However, to the best of our knowledge, no report has demonstrated the effect of PFE on endometriosis.…”

Section: Discussionmentioning

confidence: 99%

Anti‐Endometriotic Effects of Pueraria Flower Extract in Human Endometriotic Cells and Mice

et al. 2017

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Pueraria flowers have been used as a vegetable and an ingredient for tea and jelly. In this study, we investigated the effects of Pueraria flower extract (PFE) on endometriosis, a common gynaecological disease characterised by local sterile inflammation of peritoneal cavity. PFE suppressed the adhesion of human endometriotic cells 11Z and 12Z to human mesothelial Met5A cells. In addition, PFE significantly inhibited the migration of 11Z and 12Z cells as shown by wound-healing and transwell migration assays. PFE reduced the protein and mRNA levels of matrix metalloproteinase (MMP)-2 and MMP-9 in endometriotic cells. Moreover, extracellular signal-regulated kinase (ERK)1/2 was activated by PFE treatment, and an ERK1/2 inhibitor, PD98059, significantly inhibited PFE-inhibited cell migration in endometriotic cells. Furthermore, PFE significantly suppressed endometriotic lesion formation in a mouse model. These data suggest that Pueraria flower is a potential anti-endometriotic agent for the inhibition of endometriotic cell adhesion, migration, and MMP expression.

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Section: Discussionmentioning

confidence: 99%

Anti‐Endometriotic Effects of Pueraria Flower Extract in Human Endometriotic Cells and Mice

et al. 2017

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“…We previously reported preliminary findings demonstrating the anti-obesity effect of PFE in obese humans ( Kamiya et al, 2011 ). Another clinical study revealed that it reduces visceral fat area with no sexual dimorphism ( Kamiya et al, 2012 ). We also reported that PFE exerts anti-obesity and anti-fatty liver effects in high-fat diet-fed mice.…”

Section: Introductionmentioning

confidence: 99%

The Isoflavone-Rich Fraction of the Crude Extract of the Puerariae Flower Increases Oxygen Consumption and BAT UCP1 Expression in High-Fat Diet-Fed Mice

Kamiya¹,

Nagamine²,

Sameshima-Kamiya³

et al. 2012

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Puerariae flower extract (PFE) is a crude extract of the Kudzu flower. Previous studies have shown that PFE supplementation exerts anti-obesity and anti-fatty liver effects in high-fat diet-fed mice. In this study, we aimed to identify the PFE components responsible for these effects and to determine their influence on energy expenditure and uncoupling protein 1 (UCP1) expression. Experiments were conducted on C57BL/6J male mice classified into 3 groups: (1) high-fat diet-fed (HFD), (2) high-fat diet-fed given PFE (HFD + PFE), and (3) high-fat diet-fed given the PFE isoflavone-rich fraction (HFD + ISOF). All groups were fed for 42 days. The HFD + PFE and HFD + ISOF groups showed significant resistance to increases in body weight, hepatic triglyceride level, and visceral fat compared to the HFD group. These groups also exhibited significant increases in oxygen consumption and UCP1-positive brown adipose tissue (BAT) area. Our results demonstrate that the active ingredients in PFE are present in the ISOF and that these compounds may increase energy expenditure by upregulation of BAT UCP1 expression. These findings provide valuable information regarding the anti-obesity effects of isoflavones.

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“…In China, tea made from dried Kudzu flowers is widely consumed [1], and PFE is consumed as a nutritional supplement for the treatment of hangovers and obesity in Japan. We previously reported that PFE exerts anti-obesity effects in humans and mice fed high-fat diets [2][3][4]. In addition, we also reported that the isoflavone-rich fraction of dietary PFE exhibits anti-obesity effect in mice fed high-fat diets [5].…”

Section: Introductionmentioning

confidence: 85%

Evaluation of the Estrogenic Activity of Pueraria (Kudzu) Flower Extract and Its Major Isoflavones Using ER-Binding and Uterotrophic Bioassays

Kamiya¹,

Takano²,

Yayoi³

et al. 2013

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Pueraria flower extract (PFE) is a hot water extract of the Kudzu flower (Pueraria thomsonii). Tea made from driedKudzu flower is widely used in China, and PFE is utilized as a nutritional supplement in Japan. PFE contains unique isoflavones such as 6-hydroxygenistein 6,7-di-O-glucoside (6HGDG), tectorigenin 7-O-xylosylglucoside (TGXG), and tectoridin. 6HGDG is known to be metabolized into 6-hydroxygenistein, and TGXG and tectoridin are known to be metabolized into tectorigenin in the digestive tract. Isoflavones typically mimic the effects β-estradiol has on estrogen receptors (ERs) and may influence the female genital system in the case of excessive intake. As a result, the upper limit of safe daily consumption of soy isoflavones has been enforced in Japan. In the present study, ER-binding assays were performed using the EnBio estrogen receptor/cofactor assay system to compare the estrogenic activity of 6-hydroxygenistein and tectorigenin to that of the soy isoflavone genistein. In addition, uterotrophic bioassays were performed to investigate the estrogenic effects of PFE in vivo. The ER-binding assays revealed that the ER-binding affinities of 6-hydroxygenistein and tectorigenin were approximately 0.01 -0.04 that of genistein. Soy isoflavone products also induced an increase in uterine wet and blotted weight at doses of 500 mg/kg and 1000 mg/kg, whereas PFE did not cause adverse estrogenic effects, even at a dose of 1000 mg/kg. Based on these results, PFE does not appear to contain compounds with strong estrogenic activity or cause adverse estrogenic effects in vivo. Importantly, the results of this study confirm the safety of PFE as a food supplement.

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The Crude Extract fromPuerariaeFlower Exerts Antiobesity and Antifatty Liver Effects in High-Fat Diet-Induced Obese Mice

Cited by 15 publications

References 25 publications

Anti‐Endometriotic Effects of Pueraria Flower Extract in Human Endometriotic Cells and Mice

Anti‐Endometriotic Effects of Pueraria Flower Extract in Human Endometriotic Cells and Mice

The Isoflavone-Rich Fraction of the Crude Extract of the Puerariae Flower Increases Oxygen Consumption and BAT UCP1 Expression in High-Fat Diet-Fed Mice

Evaluation of the Estrogenic Activity of Pueraria (Kudzu) Flower Extract and Its Major Isoflavones Using ER-Binding and Uterotrophic Bioassays

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