The Crosstalk Between Saliva Bacteria and Fungi in Early Childhood Caries

Tu, Ye; Zhou, Zhixiang; Shu, Chang; Zhou, Yuan; Zhou, Xuedong

doi:10.3389/fcimb.2022.845738

Cited by 8 publications

(9 citation statements)

References 53 publications

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“…Dental plaque of severe ECC subjects is harbored by an increased streptococci (particularly S. mutans ), Veillonella and Prevotella , certain Lactobacillus / Scardovia species, and a decreased level of Actinomyces ( Xiao et al, 2018a ). Significant alteration in salivary fungal communities in severe ECC/ECC children is observed compared with the caries-free controls, and the fungal community is distinguished into five types based on the different oral health status, which significantly affect the bacterial profile ( Tu et al, 2022 ).…”

Section: Interactions Between C Albicans and Oral ...mentioning

confidence: 99%

Cross-kingdom interaction between Candida albicans and oral bacteria

Ren

Zhou

et al. 2022

Front. Microbiol.

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Candida albicans is a symbiotic fungus that commonly colonizes on oral mucosal surfaces and mainly affects immuno-compromised individuals. Polymicrobial interactions between C. albicans and oral microbes influence the cellular and biochemical composition of the biofilm, contributing to change clinically relevant outcomes of biofilm-related oral diseases, such as pathogenesis, virulence, and drug-resistance. Notably, the symbiotic relationships between C. albicans and oral bacteria have been well-documented in dental caries, oral mucositis, endodontic and periodontal diseases, implant-related infections, and oral cancer. C. albicans interacts with co-existing oral bacteria through physical attachment, extracellular signals, and metabolic cross-feeding. This review discusses the bacterial–fungal interactions between C. albicans and different oral bacteria, with a particular focus on the underlying mechanism and its relevance to the development and clinical management of oral diseases.

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Section: Interactions Between C Albicans and Oral ...mentioning

confidence: 99%

Cross-kingdom interaction between Candida albicans and oral bacteria

Ren

Zhou

et al. 2022

Front. Microbiol.

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“…These interactions can affect the host through pathogenesis induced by ecological shifts (Balakrishnan et al, 2021). However, previous studies have generally limited their focus to interkingdom interactions at the genus level or specific microbial species (Kim et al, 2021; Krzyściak et al, 2017; Tu et al, 2022). Therefore, further investigations are necessary to enhance our understanding of interkingdom microbial signaling and their interactions with host risk factors in caries development.…”

Section: Introductionmentioning

confidence: 99%

Role of socioeconomic factors and interkingdom crosstalk in the dental plaque microbiome in early childhood caries

Khan,

de Jesus,

Mittermuller

et al. 2024

Preprint

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Early childhood caries (ECC) is influenced by microbial and host factors, including social, behavioral, and oral health. In this cross-sectional study, we analyzed interkingdom dynamics in the dental plaque microbiome and its association with host variables. The samples collected from the preschool children underwent 16S rRNA and ITS1 rRNA gene sequencing. The questionnaire data were analyzed for social determinants of oral health. The results indicated a significant enrichment of Streptococcus mutans and Candida dubliniensis in ECC samples, in contrast to Neisseria oralis in caries-free children. Our interkingdom correlation analysis revealed that Candida dubliniensis was strongly correlated with both Neisseria bacilliformis and Prevotella veroralis in ECC. Additionally, ECC showed significant associations with host variables, including oral health status, age, place of residence, and mode of childbirth. This study provides empirical evidence associating the oral microbiome with socioeconomic and behavioral factors in relation to ECC, offering insights for developing targeted prevention strategies.

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“…It has the unique ability to ferment dietary sugars, especially sucrose, and generate organic acids as a byproduct that demineralizes tooth surfaces, producing extracellular polysaccharides (EPS) that promote cariogenic biofilm formation act against host defense (Klein et al, 2009;Chan et al, 2020). The interaction between oral bacteria and fungi in caries development has been observed in many studies (Peleg et al, 2010;Azzam et al, 2020;Cui et al, 2021;Tu et al, 2022). Candida albicans has been frequently found when there are high levels of S. mutans in caries, particularly earlychildhood caries (ECC; Raja et al, 2010;Xiao et al, 2018a,b;Cui et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Candida albicans has been frequently found when there are high levels of S. mutans in caries, particularly early-childhood caries (ECC; Raja et al, 2010 ; Xiao et al, 2018a , b ; Cui et al, 2021 ). Experiments have demonstrated a synergistic effect between S. mutans and C. albicans in vitro and in vivo ( Harriott and Noverr, 2011 ; Metwalli et al, 2013 ; Falsetta et al, 2014 ; Cui et al, 2021 ; Garcia et al, 2021 ; Tu et al, 2022 ). The presence of C. albicans facilitates S. mutans biofilm formation and accumulation, whereas S. mutans enhances hyphal growth of C. albicans in the presence of sucrose, mediated through sugar-based synergistic interactions, notably between glucosyltransferase B (gtfB) and other factors ( Hwang et al, 2015 , 2017 ; Chan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of Stevioside on Streptococcus mutans and Candida albicans dual-species biofilm

Guo

Yang²,

Zhou³

et al. 2023

Front. Microbiol.

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IntroductionStreptococcus mutans is the most prevalent biofilm-forming pathogen in dental caries, while Candida albicans is often detected in the presence of S. mutans.MethodsWe aimed to evaluate the anti-caries effect of stevioside in medium trypticase soy broth (TSB) with or without sucrose supplementation compared with the same sweetness sucrose and xylitol in a dual-species model of S. mutans and C. albicans, based on planktonic growth, crystal violet assay, acid production, biofilm structural imaging, confocal laser scanning microscopy, and RNA sequencing.ResultsOur results showed that compared with sucrose, stevioside significantly inhibited planktonic growth and acid production, changed the structure of the mixed biofilm, and reduced the viability of biofilm and the production of extracellular polysaccharides in dual-species biofilm. Through RNA-seq, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway impact analysis showed that stevioside decreased sucrose metabolism and increased galactose and intracellular polysaccharide metabolism in S. mutans, and decreased genes related to GPI-modified proteins and secreted aspartyl proteinase (SAP) family in C. albicans. In contrast to xylitol, stevioside also inhibited the transformation of fungal morphology of C. albicans, which did not form mycelia and thus had reduced pathogenicity. Stevioside revealed a superior suppression of dual-species biofilm formation compared to sucrose and a similar anti-caries effect with xylitol. However, sucrose supplementation diminished the suppression of stevioside on S. mutans and C. albicans.ConclusionsOur study is the first to confirm that stevioside has anticariogenic effects on S. mutans and C. albicans in a dual-species biofilm. As a substitute for sucrose, it may help reduce the risk of developing dental caries.

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The Crosstalk Between Saliva Bacteria and Fungi in Early Childhood Caries

Cited by 8 publications

References 53 publications

Cross-kingdom interaction between Candida albicans and oral bacteria

Cross-kingdom interaction between Candida albicans and oral bacteria

Role of socioeconomic factors and interkingdom crosstalk in the dental plaque microbiome in early childhood caries

Inhibitory effects of Stevioside on Streptococcus mutans and Candida albicans dual-species biofilm

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